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Invitrogen
Human ZNF225 (aa 97-122) Control Fragment Recombinant Protein
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Product Details
RP-107949
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
GLFSHQTWEQISSDLTRFQDSMVNSF
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (38%), Rat (38%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-67310. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krueppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. Zinc finger protein 225 (ZNF225) is a 706 amino acid member of the Krueppel C2H2-type zinc-finger protein family. Localized to the nucleus, ZNF225 contains eighteen C2H2-type zinc fingers and one KRAB domain through which it is thought to be involved in DNA-binding and transcriptional regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Zinc finger protein 225; ZN225
Gene Aliases: ZNF225
UniProt ID: (Human) Q9UK10
Entrez Gene ID: (Human) 7768
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