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Invitrogen
Human ZNF446 (aa 336-399) Control Fragment Recombinant Protein
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Product Details
RP-101118
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
QCGRGFDWKSVFVIHHRTHTSGPGVQSPGLATGESTEKPPQGEVAFPHHPRRSLTGPRSYPCEE
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
1M urea/PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (38%), Rat (38%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-140015. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
ZNF446 contains a KRAB and three C(2)H(2) zinc fingers. ZNF446 is a transcription repressor when fused to GAL4 DNA-binding domain and co-transfected with VP-16. Overexpression of ZNF446 in COS-7 cells inhibits the transcriptional activities of SRE.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ20626; Zinc finger protein 446; Zinc finger protein with KRAB and SCAN domains 20
Gene Aliases: ZKSCAN20; ZNF446; ZSCAN30; ZSCAN52
UniProt ID: (Human) Q9NWS9
Entrez Gene ID: (Human) 55663
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