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Invitrogen
Human ZNF460 (aa 525-559) Control Fragment Recombinant Protein
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Product Details
RP-107504
Applications
Tested Dilution
Publications
Product Specifications
Species
Human
Expression System
E. coli
Amino acid sequence
IHTESSPVSAVNMETPSIAAHSSSLDINGFIVEET
Tag
His-ABP-tag
Class
Recombinant
Type
Protein
Purity
>80% by SDS-PAGE and Coomassie blue staining
Conjugate
Form
Liquid
Concentration
≥5.0 mg/mL
Purification
purified
Storage buffer
PBS/1M urea, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Product Specific Information
Highest antigen sequence indentity to the following orthologs: Mouse (34%), Rat (34%).
This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-66847. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.
Target Information
Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Kruppeltype DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZNF460 (zinc finger protein 460), also known as HZF8 or ZNF272, is a 562 amino acid nuclear protein that is ubiquitously expressed at low levels and expressed at high levels in pancreas and liver. Belonging to the Krueppel C2 H2-type zinc-finger protein family, ZNF460 contains 11 C2 H2-type zinc fingers and a KRAB domain. Like other zinc-fingers, ZNF460 is also suggested to be involved in transcriptional regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Zinc finger protein 272; Zinc finger protein 460; Zinc finger protein HZF8
Gene Aliases: HZF8; ZNF272; ZNF460
UniProt ID: (Human) Q14592
Entrez Gene ID: (Human) 10794
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