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Invitrogen
Mouse EphB3 His-tag Recombinant Protein
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Product Details
RP-88235
Product Specifications
Species
Mouse
Expression System
HEK293 cells
Amino acid sequence
Mouse EPHB3, amino acids Met1-Thr537 (Accession # NP_034273.1) with a C-terminal His-tag
Tag
His-tag
Molecular weight
63 kDa
Class
Recombinant
Type
Protein
Purity
>94% as determined by SDS-PAGE.
Endotoxin concentration
<1.0 EU/µg
Activity
Measured by its binding ability in a functional ELISA. Immobilized mouse EphB3 at 2 µg/ml (100 µL/well) can bind mouse EFNB1 with a linear range of 0.1-12.5 ng/mL.
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
20mM tris, pH 7.5, with 0.01% mannitol, 150mM NaCl, 5-8% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
It is recommended that the protein be aliquoted for optimal storage.
It is recommended that sterile water (800 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.
Concentration is measured by UV.
Target Information
EPHB3 is a receptor tyrosine kinase that belongs to the ephrin receptor family. Members of the Eph family of kinases play important roles in diverse biological processes including nervous system development, angiogenesis and neural synapsis formation and maturation. The ephrin ligands and Eph receptors display reciprocal expression in vivo. Developing and adult neural tissue express nearly all of the Eph receptors and ephrin ligands. Ephs and ephrins play a significant role in angiogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Developmental kinase 5; Ephrin type-B receptor 3; mDK-5; Tyrosine-protein kinase receptor SEK-4
Gene Aliases: AW456895; Cek10; Ephb3; Etk2; HEK2; MDK5; Sek4; Tyro6
UniProt ID: (Mouse) P54754
Entrez Gene ID: (Mouse) 13845
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