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Invitrogen
Mouse Fc gamma RI (CD64) His-tag Recombinant Protein
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Product Details
A42617
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Expression System
HEK293 cells
Amino acid sequence
Mouse FCGR1, amino acids Met1-Pro297 (Accession # NP_034316.1) with a C-terminal His-tag
Tag
His-tag
Molecular weight
32 kDa
Class
Recombinant
Type
Protein
Purity
>95 % as determined by SDS-PAGE. >90 % as determined by SEC-HPLC
Endotoxin concentration
<1.0 EU/µg
Activity
1.Immobilized Rituximab (IgG1) at 1 µg/mL can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein, the EC50 is 16-35 ng/mL.
2.Immobilized Anti-CD3 antibody, mouse IgG2a at 2 µg/mL (100 µL/well) can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein, the EC50 is 0.8-2.4 ng/mL(QC tested).
3.Loaded Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).
4.Loaded Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 0.28 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).
5.Captured Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
6.Captured Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.69 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
7.The purity of Mouse Fc gamma RI (CD64) His-tag Recombinant Protein is more than 95% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
8.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) on ProA Biosensor, can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein with an affinity constant of 3.46 nM as determined in BLI assay (Sartorius Octet RED384)(QC tested).
2.Immobilized Anti-CD3 antibody, mouse IgG2a at 2 µg/mL (100 µL/well) can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein, the EC50 is 0.8-2.4 ng/mL(QC tested).
3.Loaded Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).
4.Loaded Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on His1K Biosensor, can bind Nivolumab (IgG4) with an affinity constant of 0.28 µM as determined in a BLI assay (Sartorius Octet Red384) (Routinely tested).
5.Captured Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on Anti-His Chip can bind Bevacizumab (IgG1) with an affinity constant of 0.12 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
6.Captured Mouse Fc gamma RI (CD64) His-tag Recombinant Protein on Anti-His Chip can bind Nivolumab (IgG4) with an affinity constant of 0.69 µM as determined in an SPR assay (Biacore T200) (Routinely tested).
7.The purity of Mouse Fc gamma RI (CD64) His-tag Recombinant Protein is more than 95% and the molecular weight of this protein is around 40-50 kDa verified by SEC-MALS.
8.Loaded Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (OKT3) on ProA Biosensor, can bind Mouse Fc gamma RI (CD64) His-tag Recombinant Protein with an affinity constant of 3.46 nM as determined in BLI assay (Sartorius Octet RED384)(QC tested).
Conjugate
Form
Lyophilized
Purification
purified
Storage buffer
PBS, pH 7.4, with 5% mannitol, 5% trehalose, 0.01% Tween 80
Contains
no preservative
Storage conditions
-20°C or -80°C if preferred
Shipping conditions
Ambient
Product Specific Information
Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.
Target Information
CD64 (FCGR1A) encodes a protein that plays an important role in the immune response. CD64 is a high-affinity Fc-gamma receptor and is one of three related gene family members located on chromosome 1. CD64 plays an important role in the immune response and its dysfunction has been studied in cervical adenitis and tetrasomy 21.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD64; CD64 antigen; Fc-gamma receptor 1; Fc-gamma RI; FcgRI; FcRI; FLJ18345; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; IgG high affinity Fc receptor; RP11-196G18.2
Gene Aliases: AI323638; AV092959; CD64; Fcg1; FcgammaRI; Fcgr1; IGGHAFC
UniProt ID: (Mouse) P26151
Entrez Gene ID: (Mouse) 14129
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