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Product Details
RMIL310
Applications
Tested Dilution
Publications
Product Specifications
Species
Mouse
Expression System
E. coli
Molecular weight
15.1 kDa
Class
Recombinant
Type
Protein
Purity
>96% by SDS-PAGE and HPLC analysis
Activity
ED50 < 0.1 ng/mL; determined by dose-dependent proliferation of mouse FDC-P1 cells.
Conjugate
Form
Lyophilized
Purification
purified
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
Product Specific Information
RMIL310 contains 134 amino acid residues.
Purity: >96% pure by SDS-PAGE and HPLC analysis.
Activity: One unit of activity is <0.1 ng/mL, as determined by proliferation assay of mouse IL-3-dependent FDC-P1 cells, corresponding to a specific activity of >1x10^7 units/mg.
Use: Interleukin-3 exerts activity in the range of 0.1 to 10 ng/mL for most in vitro applications.
Reconstitution and Dilution: Reconstitute in distilled water to a concentration of 100 µg/mL. After reconstitution, dilute cytokine in a neutral buffer containing carrier protein such as 5-10% FBS or 1-4% high purity BSA (fatty acid free fraction V).
Lyophilized product is stable until expiration if stored desiccated below 0ºC. Cytokines diluted as indicated above should be divided into aliquots and stored at -70ºC. Recombinant proteins should be stored at concentrations >500 ng/mL to reduce loss of activity.
Target Information
Interleukin 3 (IL-3), also called Mast Cell Growth Factor (MCGF), Multi-Colony Stimulating Factor (multi-CSF), or Eosinophil-CSF (E-CSF), is produced by activated T cells, mast cells and eosinophils. IL-3 is a hematopoietic growth factor that stimulates colony formation of erythroid, megakaryocyte, neutrophil, eosinophil, basophil, mast cell and monocytic lineages. Most of these functions are enhanced or dependent on co-stimulation with other cytokines. Human and mouse IL-3 share only 29% sequence identity at the amino acid level. The bioactivity of IL-3 is highly species specific. For example, human IL-3 has no activity on mouse cells. IL-3 is a potent growth promoting cytokine. IL-3 is capable of supporting the proliferation of a broad range of hematopoietic cell types, and is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. IL-3 has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: BP/BPA; Colony stimulating factor multiple; Eo-CSF; Hematopoietic growth factor; IL-3; ILN; Interleukin; Interleukin 3 (colony stimulating factor; Interleukin-3; Interleukin3; Mast cell growth factor; MCGF; MGC79398; MGC79399; MGF; Multi CSF; Multilineage colony stimulating factor; multiple); Multipotential colony-stimulating factor; P cell stimulating factor; P-cell-stimulating factor; PCSA
Gene Aliases: BPA; Csfmu; HCGF; Il-3; Il3; MCGF; PSF
UniProt ID: (Mouse) P01586
Entrez Gene ID: (Mouse) 16187
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