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Mouse IL-33 Recombinant Protein, PeproTech®

Mouse IL-33 Protein in Functional Assay (Functional)

FIGURE: 1 / 1

Mouse IL-33 Protein (210-33-10UG) in Functional

Bioassay analysis of Mouse IL-33 Recombinant Protein, PeproTech® (Product # 210-33-1MG).

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Product Details

210-33-10UG

Applications

Tested Dilution

Publications

Functional Assay (Functional)

View 1 publication 1 publication

In vitro Assay (IV)

View 41 publications 41 publications

Miscellaneous PubMed (MISC)

View 13 publications 13 publications

Product Specifications

Published species

Human, Mouse, Non-human primate

Expression System

E. coli

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Storage conditions

-20°C

Shipping conditions

Ambient

Product Specific Information

210-33-1MG will be provided as 10 x 100 µg (210-33-100UG).

210-33-250UG will be provided as multiple 50 µg (210-33-50UG) and 100 µg (210-33-100UG) vials.

210-33-500UG will be provided as 5 x 100 µg (210-33-100UG).

Recombinant Murine IL-33 is a 17.5 kDa protein containing 158 amino acid residues.

This product is shipped at ambient temperature. For storage, handling and reconstitution information, please see the lot-specific Certificate of Analysis

Target Information

IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Active eosinophils regulate host defence and immune responses in colitis.

Nature

Gurtner A,Borrelli C,Gonzalez-Perez I,Bach K,Acar IE,Núñez NG,Crepaz D,Handler K,Vu VP,Lafzi A,Stirm K,Raju D,Gschwend J,Basler K,Schneider C,Slack E,Valenta T,Becher B,Krebs P,Moor AE,Arnold IC

210-33 was used in In vivo experiments to provide insights into the biology of eosinophils and highlight the crucial contribution of this cell type to intestinal homeostasis, immune regulation and host defence.

Wed Mar 01 00:00:00 UTC 2023

Delineating spatiotemporal and hierarchical development of human fetal innate lymphoid cells.

Cell research

Liu C,Gong Y,Zhang H,Yang H,Zeng Y,Bian Z,Xin Q,Bai Z,Zhang M,He J,Yan J,Zhou J,Li Z,Ni Y,Wen A,Lan Y,Hu H,Liu B

Fri Oct 01 00:00:00 UTC 2021

Heterogeneous bone-marrow stromal progenitors drive myelofibrosis via a druggable alarmin axis.

Cell stem cell

Leimkühler NB,Gleitz HFE,Ronghui L,Snoeren IAM,Fuchs SNR,Nagai JS,Banjanin B,Lam KH,Vogl T,Kuppe C,Stalmann USA,Büsche G,Kreipe H,Gütgemann I,Krebs P,Banz Y,Boor P,Tai EW,Brümmendorf TH,Koschmieder S,Crysandt M,Bindels E,Kramann R,Costa IG,Schneider RK

Thu Apr 01 00:00:00 UTC 2021

Group-2 innate lymphoid cell-dependent regulation of tissue neutrophil migration by alternatively activated macrophage-secreted Ear11.

Mucosal immunology

Panova V,Gogoi M,Rodriguez-Rodriguez N,Sivasubramaniam M,Jolin HE,Heycock MWD,Walker JA,Rana BMJ,Drynan LF,Hodskinson M,Pannell R,King G,Wing M,Easton AJ,Oedekoven CA,Kent DG,Fallon PG,Barlow JL,McKenzie ANJ

Fri Jan 01 00:00:00 UTC 2021

IL-3 is essential for ICOS-L stabilization on mast cells, and sustains the IL-33-induced RORγt⁺ T_reg generation via enhanced IL-6 induction.

Immunology

Drube S,Müller S,Weber F,Wegner P,Böttcher-Loschinski R,Gaestel M,Hutloff A,Kamradt T,Andreas N

Sat May 01 00:00:00 UTC 2021

In Activated Murine Mast Cells, NFATc2 Is Critical for the Production of Autocrine IL-3, Thereby Promoting the Expression of IL-9.

Journal of immunology (Baltimore, Md. : 1950)

Sabbaghi F,Ullner L,Bohn T,Hahlbrock J,Bopp T,Schmitt E,Klein M,Stassen M

Fri Jan 01 00:00:00 UTC 2021

Sprouty2 limits intestinal tuft and goblet cell numbers through GSK3β-mediated restriction of epithelial IL-33.

Nature communications

Schumacher MA,Hsieh JJ,Liu CY,Appel KL,Waddell A,Almohazey D,Katada K,Bernard JK,Bucar EB,Gadeock S,Maselli KM,Washington MK,Grikscheit TC,Warburton D,Rosen MJ,Frey MR

Fri Feb 05 00:00:00 UTC 2021

Sprouty2 limits intestinal tuft and goblet cell numbers through GSK3β-mediated restriction of epithelial IL-33.

Nature communications

Schumacher MA,Hsieh JJ,Liu CY,Appel KL,Waddell A,Almohazey D,Katada K,Bernard JK,Bucar EB,Gadeock S,Maselli KM,Washington MK,Grikscheit TC,Warburton D,Rosen MJ,Frey MR

Fri Feb 05 00:00:00 UTC 2021

Graft IL-33 regulates infiltrating macrophages to protect against chronic rejection.

The Journal of clinical investigation

Li T,Zhang Z,Bartolacci JG,Dwyer GK,Liu Q,Mathews LR,Velayutham M,Roessing AS,Lee YC,Dai H,Shiva S,Oberbarnscheidt MH,Dziki JL,Mullet SJ,Wendell SG,Wilkinson JD,Webber SA,Wood-Trageser M,Watkins SC,Demetris AJ,Hussey GS,Badylak SF,Turnquist HR

Thu Oct 01 00:00:00 UTC 2020

ILC2s are the predominant source of intestinal ILC-derived IL-10.

The Journal of experimental medicine

Bando JK,Gilfillan S,Di Luccia B,Fachi JL,Sécca C,Cella M,Colonna M

210-33 was used in Cell Culture to report the absence of an intestinal regulatory ILC population distinct from group 1 ILCs (ILC1s), ILC2s, and ILC3s in (1) mice bred in our animal facility; (2) mice from The Jackson Laboratory, Taconic Biosciences, and Charles River Laboratories; and (3) mice subjected to intestinal inflammation.

Mon Feb 03 00:00:00 UTC 2020

COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice.

Journal of immunology (Baltimore, Md. : 1950)

Zhou W,Zhang J,Toki S,Goleniewska K,Norlander AE,Newcomb DC,Wu P,Boyd KL,Kita H,Peebles RS

Sat Aug 15 00:00:00 UTC 2020

IL-33-induced neutrophil extracellular traps degrade fibronectin in a murine model of bronchopulmonary dysplasia.

Cell death discovery

Jin R,Xu J,Gao Q,Mao X,Yin J,Lu K,Guo Y,Zhang M,Cheng R

Tue Sep 26 00:00:00 UTC 2023

CD49a Regulates Cutaneous Resident Memory CD8⁺ T Cell Persistence and Response.

Cell reports

Bromley SK,Akbaba H,Mani V,Mora-Buch R,Chasse AY,Sama A,Luster AD

210-33 was used in In vitro experiments to study the requirement for CD49a in the formation, localization, persistence, and function of cutaneous CD8+ tissue-resident memory T cells.

Tue Sep 01 00:00:00 UTC 2020

Mouse Strain-Dependent Difference Toward the Staphylococcus aureus Allergen Serine Protease-Like Protein D Reveals a Novel Regulator of IL-33.

Frontiers in immunology

Teufelberger AR,Van Nevel S,Hulpiau P,Nordengrün M,Savvides SN,De Graeve S,Akula S,Holtappels G,De Ruyck N,Declercq W,Vandenabeele P,Hellman L,Bröker BM,Krysko DV,Bachert C,Krysko O

210-33 was used in In vitro experiments to find the underlying genetic difference between BALB/c and C57BL/6J mice leading to the different response toward SplD.

Tue Jun 22 00:00:00 UTC 2021

The IL-33-induced p38-/JNK1/2-TNFα axis is antagonized by activation of β-adrenergic-receptors in dendritic cells.

Scientific reports

Helbig C,Weber F,Andreas N,Herdegen T,Gaestel M,Kamradt T,Drube S

Mon May 18 00:00:00 UTC 2020

Establishment of bone marrow-derived M-CSF receptor-dependent self-renewing macrophages.

Cell death discovery

Nasser H,Adhikary P,Abdel-Daim A,Noyori O,Panaampon J,Kariya R,Okada S,Ma W,Baba M,Takizawa H,Yamane M,Niwa H,Suzu S

Tue Sep 26 00:00:00 UTC 2023

A Macrophage-Pericyte Axis Directs Tissue Restoration via Amphiregulin-Induced Transforming Growth Factor Beta Activation.

Immunity

Minutti CM,Modak RV,Macdonald F,Li F,Smyth DJ,Dorward DA,Blair N,Husovsky C,Muir A,Giampazolias E,Dobie R,Maizels RM,Kendall TJ,Griggs DW,Kopf M,Henderson NC,Zaiss DM

Tue Mar 19 00:00:00 UTC 2019

Extrapituitary prolactin promotes generation of Eomes-positive helper T cells mediating neuroinflammation.

Proceedings of the National Academy of Sciences of the United States of America

Zhang C,Raveney BJE,Hohjoh H,Tomi C,Oki S,Yamamura T

Tue Oct 15 00:00:00 UTC 2019

IL-33 regulates cytokine production and neutrophil recruitment via the p38 MAPK-activated kinases MK2/3.

Immunology and cell biology

McCarthy PC,Phair IR,Greger C,Pardali K,McGuire VA,Clark AR,Gaestel M,Arthur JSC

Tue Jan 01 00:00:00 UTC 2019

RelB Deficiency in Dendritic Cells Protects from Autoimmune Inflammation Due to Spontaneous Accumulation of Tissue T Regulatory Cells.

Journal of immunology (Baltimore, Md. : 1950)

Andreas N,Potthast M,Geiselhöringer AL,Garg G,de Jong R,Riewaldt J,Russkamp D,Riemann M,Girard JP,Blank S,Kretschmer K,Schmidt-Weber C,Korn T,Weih F,Ohnmacht C

Fri Nov 15 00:00:00 UTC 2019

Oncostatin M expression in the mouse uterus during early pregnancy promotes embryo implantation and decidualization.

FEBS letters

Fu T,Zheng HT,Zhang HY,Chen ZC,Li B,Yang ZM

210-33 was used in Cell Culture to study Osm high expression in the uterine epithelium from days 1 to 4 of pregnancy and in decidual cells on day 5 of pregnancy to indicate that prostaglandin E2 (PGE2 )-induced Osm may mediate embryo implantation through Il-33 and participate in decidualization via the Stat3-Egr1 pathway.

Thu Aug 01 00:00:00 UTC 2019

Runx/Cbfβ complexes protect group 2 innate lymphoid cells from exhausted-like hyporesponsiveness during allergic airway inflammation.

Nature communications

Miyamoto C,Kojo S,Yamashita M,Moro K,Lacaud G,Shiroguchi K,Taniuchi I,Ebihara T

Fri Jan 25 00:00:00 UTC 2019

IL1RL1 is dynamically expressed on Cbfb-MYH11⁺ leukemia stem cells and promotes cell survival.

Scientific reports

Wang Y,Richter L,Becker M,Amador C,Hyde RK

Mon Feb 11 00:00:00 UTC 2019

Lung Single-Cell Signaling Interaction Map Reveals Basophil Role in Macrophage Imprinting.

Cell

Cohen M,Giladi A,Gorki AD,Solodkin DG,Zada M,Hladik A,Miklosi A,Salame TM,Halpern KB,David E,Itzkovitz S,Harkany T,Knapp S,Amit I

Thu Nov 01 00:00:00 UTC 2018

IL-33 promotes the egress of group 2 innate lymphoid cells from the bone marrow.

The Journal of experimental medicine

Stier MT,Zhang J,Goleniewska K,Cephus JY,Rusznak M,Wu L,Van Kaer L,Zhou B,Newcomb DC,Peebles RS

Tue Jan 02 00:00:00 UTC 2018

IL-33 promotes the egress of group 2 innate lymphoid cells from the bone marrow.

The Journal of experimental medicine

Stier MT,Zhang J,Goleniewska K,Cephus JY,Rusznak M,Wu L,Van Kaer L,Zhou B,Newcomb DC,Peebles RS

Tue Jan 02 00:00:00 UTC 2018

The PGI₂ Analog Cicaprost Inhibits IL-33-Induced Th2 Responses, IL-2 Production, and CD25 Expression in Mouse CD4⁺ T Cells.

Journal of immunology (Baltimore, Md. : 1950)

Zhou W,Zhang J,Toki S,Goleniewska K,Johnson MO,Bloodworth MH,Newcomb DC,Peebles RS

Mon Oct 01 00:00:00 UTC 2018

The p38-MK2/3 Module Is Critical for IL-33-Induced Signaling and Cytokine Production in Dendritic Cells.

Journal of immunology (Baltimore, Md. : 1950)

Göpfert C,Andreas N,Weber F,Häfner N,Yakovleva T,Gaestel M,Kamradt T,Drube S

Thu Feb 01 00:00:00 UTC 2018

Vesicle-associated membrane protein 7-mediated eosinophil degranulation promotes allergic airway inflammation in mice.

Communications biology

Willetts L,Felix LC,Jacobsen EA,Puttagunta L,Condjella RM,Zellner KR,Ochkur SI,Kim JD,Luo H,Lee NA,Lee JJ,Moqbel R,Lacy P

Sat Jan 11 00:00:00 UTC 2020

IL-4 enhances survival of in vitro-differentiated mouse basophils through transcription-independent signaling downstream of PI3K.

Cell death & disease

Reinhart R,Kaufmann T

Mon Jun 18 00:00:00 UTC 2018

Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.

Cell

Wang S,Xia P,Chen Y,Qu Y,Xiong Z,Ye B,Du Y,Tian Y,Yin Z,Xu Z,Fan Z

210-33 was used in Cell Culture to identify a regulatory subpopulation of innate lymphoid cells that exists in the gut, and harbour a unique gene identity.

Thu Sep 21 00:00:00 UTC 2017

Regulatory Innate Lymphoid Cells Control Innate Intestinal Inflammation.

Cell

Wang S,Xia P,Chen Y,Qu Y,Xiong Z,Ye B,Du Y,Tian Y,Yin Z,Xu Z,Fan Z

210-33 was used in Cell Culture to identify a regulatory subpopulation of innate lymphoid cells that exists in the gut, and harbour a unique gene identity.

Thu Sep 21 00:00:00 UTC 2017

Epidermal Growth Factor Receptor Expression Licenses Type-2 Helper T Cells to Function in a T Cell Receptor-Independent Fashion.

Immunity

Minutti CM,Drube S,Blair N,Schwartz C,McCrae JC,McKenzie AN,Kamradt T,Mokry M,Coffer PJ,Sibilia M,Sijts AJ,Fallon PG,Maizels RM,Zaiss DM

Tue Oct 17 00:00:00 UTC 2017

The Innate Cytokines IL-25, IL-33, and TSLP Cooperate in the Induction of Type 2 Innate Lymphoid Cell Expansion and Mucous Metaplasia in Rhinovirus-Infected Immature Mice.

Journal of immunology (Baltimore, Md. : 1950)

Han M,Rajput C,Hong JY,Lei J,Hinde JL,Wu Q,Bentley JK,Hershenson MB

Tue Aug 15 00:00:00 UTC 2017

Dectin-1 signaling inhibits osteoclastogenesis via IL-33-induced inhibition of NFATc1.

Oncotarget

Zhu X,Zhao Y,Jiang Y,Qin T,Chen J,Chu X,Yi Q,Gao S,Wang S

210-33 was used in Cell Culture to study the underlying mechanisms of how the agonist of dectin-1 inhibirs osteoclastogenesis and determine that curdlan potently inhibited RANKL-induced osteoclast differentiation and the resultant bone resorption.

Tue Aug 08 00:00:00 UTC 2017

IL-33 regulates the IgA-microbiota axis to restrain IL-1α-dependent colitis and tumorigenesis.

The Journal of clinical investigation

Malik A,Sharma D,Zhu Q,Karki R,Guy CS,Vogel P,Kanneganti TD

Thu Dec 01 00:00:00 UTC 2016

Recent thymic emigrants are tolerized in the absence of inflammation.

The Journal of experimental medicine

Friesen TJ,Ji Q,Fink PJ

Mon May 30 00:00:00 UTC 2016

IL13 activates autophagy to regulate secretion in airway epithelial cells.

Autophagy

Dickinson JD,Alevy Y,Malvin NP,Patel KK,Gunsten SP,Holtzman MJ,Stappenbeck TS,Brody SL

210-33 was used in In vivo experiments to suggest that the regulation of autophagy by Th2 cytokines is cell-context dependent.

Tue Dec 13 00:00:00 UTC 2016

IL-33 delivery induces serous cavity macrophage proliferation independent of interleukin-4 receptor alpha.

European journal of immunology

Jackson-Jones LH,Rückerl D,Svedberg F,Duncan S,Maizels RM,Sutherland TE,Jenkins SJ,McSorley HJ,Bénézech C,MacDonald AS,Allen JE

Sat Oct 01 00:00:00 UTC 2016

Intratumorally Establishing Type 2 Innate Lymphoid Cells Blocks Tumor Growth.

Journal of immunology (Baltimore, Md. : 1950)

Kim J,Kim W,Moon UJ,Kim HJ,Choi HJ,Sin JI,Park NH,Cho HR,Kwon B

Tue Mar 01 00:00:00 UTC 2016

MK2/3 Are Pivotal for IL-33-Induced and Mast Cell-Dependent Leukocyte Recruitment and the Resulting Skin Inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Drube S,Kraft F,Dudeck J,Müller AL,Weber F,Göpfert C,Meininger I,Beyer M,Irmler I,Häfner N,Schütz D,Stumm R,Yakovleva T,Gaestel M,Dudeck A,Kamradt T

Tue Nov 01 00:00:00 UTC 2016

MicroRNA-487b Is a Negative Regulator of Macrophage Activation by Targeting IL-33 Production.

Journal of immunology (Baltimore, Md. : 1950)

Xiang Y,Eyers F,Herbert C,Tay HL,Foster PS,Yang M

Fri Apr 15 00:00:00 UTC 2016

The IL-33/ST2 pathway contributes to intestinal tumorigenesis in humans and mice.

Oncoimmunology

Mertz KD,Mager LF,Wasmer MH,Thiesler T,Koelzer VH,Ruzzante G,Joller S,Murdoch JR,Brümmendorf T,Genitsch V,Lugli A,Cathomas G,Moch H,Weber A,Zlobec I,Junt T,Krebs P

210-33 was used in Flow cytometry/Cell sorting to reveal a tumor-promoting role of IL-33/ST2 signaling in CRC.

Wed Mar 30 00:00:00 UTC 2022

The transcription factor Bcl11b is specifically expressed in group 2 innate lymphoid cells and is essential for their development.

The Journal of experimental medicine

Yu Y,Wang C,Clare S,Wang J,Lee SC,Brandt C,Burke S,Lu L,He D,Jenkins NA,Copeland NG,Dougan G,Liu P

Mon Jun 01 00:00:00 UTC 2015

Spred1, a Suppressor of the Ras-ERK Pathway, Negatively Regulates Expansion and Function of Group 2 Innate Lymphoid Cells.

Journal of immunology (Baltimore, Md. : 1950)

Suzuki M,Morita R,Hirata Y,Shichita T,Yoshimura A

Sat Aug 01 00:00:00 UTC 2015

Composition, Development, and Function of Uterine Innate Lymphoid Cells.

Journal of immunology (Baltimore, Md. : 1950)

Doisne JM,Balmas E,Boulenouar S,Gaynor LM,Kieckbusch J,Gardner L,Hawkes DA,Barbara CF,Sharkey AM,Brady HJ,Brosens JJ,Moffett A,Colucci F

Thu Oct 15 00:00:00 UTC 2015

Oxidation of the alarmin IL-33 regulates ST2-dependent inflammation.

Nature communications

Cohen ES,Scott IC,Majithiya JB,Rapley L,Kemp BP,England E,Rees DG,Overed-Sayer CL,Woods J,Bond NJ,Veyssier CS,Embrey KJ,Sims DA,Snaith MR,Vousden KA,Strain MD,Chan DT,Carmen S,Huntington CE,Flavell L,Xu J,Popovic B,Brightling CE,Vaughan TJ,Butler R,Lowe DC,Higazi DR,Corkill DJ,May RD,Sleeman MA,Mustelin T