Invitrogen

Mouse ROR1 His-tag Recombinant Protein

Mouse ROR1 His-tag Protein in SDS-PAGE (SDS-PAGE)

FIGURE: 1 / 1

Mouse ROR1 His-tag Protein (RP-88256) in SDS-PAGE

SDS-PAGE analysis of Mouse ROR1 His-tag (Product # RP-88256). The specification for purity shows: ≥ 95 % (SDS-PAGE), ≥ 95 % (SEC-HPLC).

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Product Details

RP-88256

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Product Specifications

Species

Mouse

Expression System

HEK293 cells

Amino acid sequence

Mouse ROR1, amino acids Met1-Glu403 (Accession # NP_038873.2) with a C-terminal His-tag

Tag

His-tag

Molecular weight

59.4 kDa

Class

Recombinant

Type

Protein

Purity

>95% as determined by SDS-PAGE.

Endotoxin concentration

<1.0 EU/µg

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

PBS, pH 7.4, with 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

Product Specific Information

It is recommended that the protein be aliquoted for optimal storage.

It is recommended that sterile water (800 µL) be added to the vial to prepare a stock solution of 1.25 mg/mL.

Concentration is measured by UV.

Target Information

Receptor-tyrosine-kinase-like orphan receptor 1 (ROR1) is a tumor-associated, surface protein predominantly expressed during embryogenesis, where it is involved in organ morphogenesis, nervous system development, and neural progenitor cell maintenance and survival. Virtually absent from normal pediatric and adult tissues, with the exception of low-level expression in a subset of immature b cell precursors known as hematogones and adipocytes, ROR1 is notably overexpressed, and considered a survival factor, in a number of B lymphoid and epithelial malignancies: including chronic lymphocytic leukemia (CLL), mantle cell lymphoma (MCL), acute lymphoblastic leukemia (ALL), marginal zone lymphoma, lung adenocarcinoma. First identified during PCR-based cloning of a human neuroblastoma cell line in search of tyrosine kinases similar to tropomyosin-receptor-kinase (Trk) neurotropic receptors, ROR1, along with the related receptor tyrosine kinase (RTK) ROR2, was catalogued as an "orphan" receptor due to the fact its related ligand remained elusive. Wnt-5a has since been suggested as a candidate ligand for ROR1, and ROR1 has been implicated to function as a pseudokinase, promoting proliferation and resistance to apoptosis in cancer cells through interaction with Wnt-5a, and TCL1-co-activation of AKT. ROR1 is expressed as a glycoprotein containing extracellular immunoglobulin (Ig)-like, Frizzled, and Kringle domains, as well as an intracellular region containing a tyrosine kinase domain.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Inactive tyrosine-protein kinase transmembrane receptor ROR1; MGC99659; mROR1; neurotrophic tyrosine kinase, receptor related 1; Neurotrophic tyrosine kinase, receptor-related 1; T-ROR1; tyrosine-protein kinase transmembrane receptor ROR1