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Product Details
210-33R-50UG
Product Specifications
Species
Mouse
Expression System
Baculovirus
Amino acid sequence
SKSSWGLENE ALIVRCPQRG RSTYPVEWYY SDTNESIPTQ KRNRIFVSRD RLKFLPARVE DSGIYACVIR SPNLNKTGYL NVTIHKKPPS CNIPDYLMYS TVRGSDKNFK ITCPTIDLYN WTAPVQWFKN CKALQEPRFR AHRSYLFIDN VTHDDEGDYT CQFTHAENGT NYIVTATRSF TVEEKGFSMF PVITNPPYNH TMEVEIGKPA SIACSACFGK GSHFLADVLW QINKTVVGNF GEARIQEEEG RNESSSNDMD CLTSVLRITG VTEKDLSLEY DCLALNLHGM IRHTIRLRRK QPIDHRGGPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGK
Tag
Fc-tag
Molecular weight
61.2 kDa
Class
Recombinant
Type
Protein
Purity
= 95% by SDS-PAGE gel and HPLC analyses.
Endotoxin concentration
<1 EU/µg
Activity
Determined by its ability to bind immobilized Murine IL-33 in a functional ELISA.
Conjugate
Form
Lyophilized
Purification
SDS-PAGE, HPLC
Storage conditions
-20°C
Shipping conditions
Ambient
Product Specific Information
IL-33R, also known as ST2, is a receptor for IL-33. It is a member of the IL-1 receptor family of innate receptors. Its
extracellular domain consists of 3 Ig-like domains that directly interact with its co-receptor IL-1RAcP, and the
ligand alarmin, IL-33. IL-33 ligation results in the association of IL-33R complex with its adaptor proteins MyD88,
IRAK1, IRAK4 and TRAF6, and activation of the NF-?B, and MAPK pathways. Recombinant Murine sIL-33 Receptor Fc is a 61.2 kDa protein containing 539 amino acid residues consisting of
the extracellular domain of the mature sequence, as well as a 230 C-terminal Fc region linked by two glycines.
Target Information
IL-33 (Interleukin-33) is a 270 amino acid, highly divergent protein belonging to the IL-1 family with an IL-1-like C-terminal domain. IL-33 is a dual function protein that may function both as a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. IL-33 binds to and signals through IL1RL1/ST2 and its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6. IL-33 activates NF-kappaB and MAP kinases, and drives production of TH2-associated cytokines from in vitro polarized TH2 cells. In vivo, IL-33 induces the expression of IL-4, IL-5, and IL-13 and leads to severe pathological changes in mucosal organs. IL-33 is proteolytically converted to a mature form by CASP1 and is highly expressed in high endothelial venules found in tonsils, Peyer's patches and mesenteric lymph nodes and is almost undetectable in placenta. Prolonged IL-33 treatment of mice led to the development of eosinophilia, splenomegaly, and severe pathological changes in mucosal organs such as lungs, esophagus and small intestine. Recent experiments have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine and an intracellular nuclear factor with transcriptional regulatory properties. Despite its predicted molecular weight, IL-33 will often run at higher molecular weight in SDS-PAGE. Studies have shown that IL-33 can also co-localize with heterochromatin and possesses transcriptional repressor activities, indicating that IL-33 may function as both a proinflammatory cytokine, and an intracellular nuclear factor with transcriptional regulatory properties.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: H-IL-33; IL-33; ILN; Interleukin; Interleukin-1 receptor-like 1; Interleukin-33; Interleukin-33 receptor alpha chain; Interleukin33; Lymphocyte antigen 84; nuclear factor from high endothelial venules; Protein ST2; Protein T1; RP11-575C20.2
Gene Aliases: 9230117N10Rik; DER4; Fit-1; Il-33; Il1f11; Il1rl1; Il33; Ly84; NF-HEV; St2; St2-rs1; ST2L; Ste2; T1; T1/ST2
UniProt ID: (Mouse) P14719, (Mouse) Q8BVZ5
Entrez Gene ID: (Mouse) 17082, (Mouse) 77125
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