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          • Proteins & Peptides ›
          • SARS-CoV-2 Spike Protein RBD Proteins

          Invitrogen

          SARS-CoV-2 Spike Protein (RBD) His-Avi Tag Recombinant Protein, Biotin

          View all (143) SARS-CoV-2 Spike Protein RBD proteins

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          Cite SARS-CoV-2 Spike Protein (RBD) His-Avi Tag Recombinant Protein, Biotin

          • Testing Data (1)
          SARS-CoV-2 Spike Protein (RBD) His-Avi Tag Protein in SDS-PAGE (SDS-PAGE)
          Group 53 Created with Sketch.
          SARS-CoV-2 Spike Protein (RBD) His-Avi Tag Protein in SDS-PAGE (SDS-PAGE)
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          SARS-CoV-2 Spike Protein (RBD) His-Avi Tag Protein (RP-88386) in SDS-PAGE

          SDS-PAGE analysis of SARS-CoV-2 Spike Protein (RBD) His-Avi Tag (Product # RP-88386). The specification for purity shows: > 95 % (SDS-PAGE). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          SARS-CoV-2 Spike Protein (RBD) His-Avi Tag Protein in SDS-PAGE (SDS-PAGE)

          Product Details

          RP-88386

          Applications
          Tested Dilution
          Publications

          ELISA (ELISA)

          Assay-dependent
          -
          Product Specifications

          Species

          Virus

          Expression System

          Baculovirus

          Amino acid sequence

          SARS-CoV-2 spike RBD, amino acids Arg319-Phe541 (Accession # YP_009724390.1) with a C-terminal Avi-tagged His-tag

          Tag

          His-tag, Avi-tag

          Molecular weight

          28.36 kDa

          Class

          Recombinant

          Type

          Protein

          Purity

          >95% as determined by SDS-PAGE.

          Endotoxin concentration

          <1.0 EU/µg

          Activity

          Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 µg/mL (100 µL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD-AVI and His Recombinant Protein, Biotinylated (RP-88386),the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD-AVI and His Recombinant Protein, Biotinylated (RP-88386) is 17-40 ng/mL.

          Conjugate

          Biotin Biotin Biotin

          Form

          Lyophilized

          Purification

          purified

          Storage buffer

          PBS, pH 7, with <0.1µg/mL BSA, 5% glycerol, 0.01% mannitol, 5-8% trehalose, 0.01% Tween 80

          Contains

          no preservative

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Wet ice

          Product Specific Information

          It is recommended that the protein be aliquoted for optimal storage.

          It is recommended that sterile water (80 µL) be added to the vial to prepare a stock solution of 0.25 mg/mL.

          Concentration is measured by UV.

          Target Information

          SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold symptoms to severe effects including death and respiratory distress. The CoV Spike (S) protein plays the most important roles in viral attachment, fusion and entry, and serves as a target for development of antibodies, entry inhibitors and vaccines. Of note is the Spike protein receptor-binding domain (RBD, S-RBD) in SARS-CoV-2 Spike protein which binds strongly to human and bat angiotensin-converting enzyme 2 (ACE2) receptors. SARS-CoV-2 RBD exhibits significantly higher binding affinity to ACE2 receptor than SARS-CoV RBD in comparison. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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