Ion Chromatography for
Pharma and Biopharma

View the basics of high-performance anion exchange (HPAE) Chromatography, it’s advantages for pharma and biopharmaceutical analysis with an overview of the relevant technical and application notes for: 

• Monosaccharides, Sialic Acids & Glycans
• Cell Culture Media & Fermentation Broths
• Counterion and Impurity Analysis
• Aminoglycosides

A glycopeptide standard for improving glycoprotein hydrolysis reaction yield for accurate monosaccharide content determination

See the application of a glycopeptide standard for improving accuracy of glycoprotein monosaccharide quantification using HPAE-PAD on a Thermo Scientific Dionex CarboPac PA20-4µm-Fast column.

HPAE-PAD analysis of N-linked glycans: improving glycan resolution

The goal of this application is to demonstrate conditions for improved resolution of sialylated N-glycans on Thermo Scientific Dionex CarboPac PA200 columns.

HPAE-PAD profiling of N-linked oligosaccharides from glycoproteins using dual-eluent generation cartridges

See the Dual EGC capability and performance for profiling N-linked oligosaccharides released from glycoproteins using high-performance anion exchange chromatography with pulsed amperometric detection (HPAE-PAD).

Determination of the Amino Acid Content of Peptides by AAA-Direct

Learn how the AAA-Direct system, which separates amino acids on a high performance anion-exchange column and directly detects them by integrated amperometry.

Analysis of Carbohydrates by High-Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD)

Here, HPAE-PAD is described as a technique and detection method for separating carbohydrates.

Improved profiling of sialylated N-linked glycans by ion chromatography-Orbitrap mass spectrometry

See HPAE-MS described as a method for improved glycan profiling.

An improved HPAE-PAD method for glycoprotein monosaccharide determination

This application demonstrates an improved HPAE-PAD assay for determination of the monosaccharide composition of a glycoprotein.

Instrument configuration for native N-linked oligosaccharide characterization by HPAE-PAD/MS

Get application installation instructions for coupling HPAE-PAD analyses to the Thermo Scientific Q Exactive family of mass spectrometers, with a focus on using this set up to profile N-linked glycans released from glycoproteins.