Add targets to multiplex kits, or design your own mix & match panels
ProcartaPlex Simplex kits are magnetic bead sets used to detect individual analytes. Create your own mix & match panel by combining multiple Simplex kits. A corresponding basic kit is required when combining simplex kits separate from any multiplex preconfigured panel.
Find your target analyte
| ProcartaPlex analytes (A) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| 4-1BB (CD137) | EPX01A-10289-901 | |||||
| 4-1BBL (CD137L) | EPX010-15901-901 | EPX010-26058-901 | ||||
| 6Ckine (CCL21) | EPX010-12384-901 | |||||
| Adiponectin | EPX01A-12032-901 | EPX01A-26038-901 | EPX01A-42032-901 | |||
| Alpha-1-Microglobulin | EPX010-12452-901 | |||||
| Alpha-synuclein [pS129] | EPX010-12361-901 | |||||
| Alpha-synuclein (total) | EPX010-12360-901 | |||||
| Amyloid beta (1–40) | EPX010-12350-901 | |||||
| Amyloid beta (1–42) | EPX010-12351-901 | |||||
| Angiogenin | EPX010-12234-901 | |||||
| Angiopoietin-1 | EPX010-12235-901 | |||||
| Angiostatin | EPX010-12237-901 | |||||
| Apolipoprotein E4 | EPX010-12344-901 | |||||
| APRIL | EPX01A-12008-901 | |||||
| Arginase-1 | EPX010-12216-901 | |||||
| AXL | EPX010-12286-901 |
| ProcartaPlex analytes (B) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| B7-H6 | EPX010-12328-901 | |||||
| BACE1 (beta-secretase 1) | EPX010-12358-901 | |||||
| BAFF | EPX01A-12007-901 | EPX01A-26096-901 | ||||
| BDNF | EPX01A-12116-901 | EPX01A-42116-901 | ||||
| Beta-2-microglobulin (B2M) | EPX010-12414-901 | |||||
| Betacellulin (BTC) | EPX01A-26042-901 | |||||
| Big ET-1 | EPX010-12266-901 | |||||
| BLC (CXCL13) | EPX01A-12147-901 | EPX01A-42147-901 | ||||
| BMP-2 | EPX010-12319-901 | |||||
| BMP-9 | EPX010-12246-901 | |||||
| BTLA (CD272) | EPX01A-12217-901 | EPX010-26052-901 |
| ProcartaPlex analytes (D-F) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| D-Dimer | EPX01A-12149-901 | |||||
| DR6 | EPX01A-15904-901 | |||||
| E-cadherin | EPX010-12315-901 | |||||
| EDA-1 | EPX01A-15903-901 | |||||
| EGF | EPX01A-12070-901 | |||||
| Elafin | EPX010-12248-901 | |||||
| EMMPRIN | EPX010-12242-901 | |||||
| ENA-78 (CXCL5) | EPX01A-12119-901 | EPX01A-26043-901 | ||||
| Endoglin (CD105) | EPX01A-12105-901 | |||||
| Endostatin | EPX010-12238-901 | |||||
| Eotaxin (CCL11) | EPX01B-12120-901 | EPX01A-26008-901 | EPX01A-36008-901 | EPX01B-42120-901 | ||
| Eotaxin-2 (CCL24) | ||||||
| Eotaxin-3 (CCL26) | EPX01A-12140-901 | |||||
| FABP-3 | EPX010-12263-901 | |||||
| Factor X | EPX01A-12152-901 | |||||
| Fas (APO) | EPX01A-10245-901 | |||||
| Fas-L | EPX01A-10260-901 | |||||
| Ferritin | EPX010-12184-901 | |||||
| Fetuin-A | EPX010-12347-901 | |||||
| FGF-2 | EPX01A-12074-901 | EPX01A-42074-901 | ||||
| FGF-21 | EPX010-12357-901 | |||||
| FGF-23 | EPX010-12204-901 | |||||
| Fibrinogen | EPX01A-12153-901 | |||||
| Flt3-ligand | EPX010-12387-901 | |||||
| Follistatin | EPX010-12244-901 | |||||
| Fractalkine (CX3CL1) | EPX01A-12121-901 |
| ProcartaPlex analytes (G-H) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| Galectin-3 | EPX01A-10279-901 | |||||
| Galectin-9 | EPX010-12331-901 | |||||
| GAPDH | EPX01A-12154-901 | |||||
| GAS6 | EPX010-12291-901 | |||||
| GCP-2 (CXCL6) | EPX010-12386-901 | |||||
| G-CSF (CSF-3) | EPX01A-12001-901 | EPX01A-26034-901 | EPX01A-36034-901 | EPX01A-42001-901 | ||
| GDF-15 | EPX010-12258-901 | |||||
| GDNF | EPX010-12340-901 | |||||
| GFAP | EPX010-12336-901 | |||||
| Ghrelin | EPX010-12192-901 | |||||
| GITR (CD357) | EPX01A-12210-901 | |||||
| GITRL | EPX01A-15902-901 | |||||
| GLP-1 | EPX010-12194-901 | |||||
| GM-CSF | EPX01A-10283-901 | EPX01A-20612-901 | EPX01A-30612-901 | EPX01A-40283-901 | ||
| gp130 (IL-6RB) | EPX010-12376-901 | |||||
| Granzyme A | EPX010-12232-901 | |||||
| Granzyme B | EPX01A-12027-901 | EPX01A-42027-901 | ||||
| GRO alpha (KC/CXCL1) | EPX01A-12122-901 | EPX01A-26031-901 | EPX01A-36031-901 | |||
| GSTA1 (Glutathione S-transferase A1) | EPX010-12450-901 | |||||
| HB-EGF | EPX010-12245-901 | |||||
| HER-2 | EPX01A-10207-901 | |||||
| HGF | EPX01A-12069-901 | |||||
| HSP60 | EPX010-12323-901 | |||||
| HVEM (CD270) | EPX01A-12218-901 |
| ProcartaPlex analytes (K-L) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| Kallikrein-6 (KLK6) | EPX010-12355-901 | |||||
| KIM-1 (TIM-e/HAVCR1) | EPX010-12445-901 | |||||
| KRT18 | EPX010-12247-901 | |||||
| Lactoferrin | EPX010-12250-901 | |||||
| LAG-3 | EPX01A-12211-901 | EPX010-26051-901 | ||||
| LAP (TGF-beta 1) | EPX010-12065-901 | |||||
| LDH-B | EPX010-12262-901 | |||||
| Leptin | EPX01A-12039-901 | EPX01A-26036-901 | EPX01A-32039-901 | |||
| LIF | EPX01A-10242-901 | EPX01A-26040-901 | ||||
| LIGHT | EPX01A-12009-901 | |||||
| LOX-1 | EPX010-12296-901 | |||||
| Lp-PLA2 | EPX010-12324-901 | |||||
| LYVE-1 | EPX010-12239-901 |
| ProcartaPlex analytes (N-P) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| NCAM-1 | EPX010-12354-901 | |||||
| Nectin-2 | EPX010-12304-901 | |||||
| Neurogranin (NRGN) | EPX010-12352-901 | |||||
| NF-H | EPX010-12337-901 | |||||
| NGAL | EPX01A-12202-901 | |||||
| NGF-beta | EPX01A-12117-901 | EPX01A-32117-901 | EPX01A-42117-901 | EPX01A-52117-901 | ||
| NRP-1 | EPX010-12243-901 | |||||
| NSE | EPX010-12335-901 | |||||
| NT5E | EPX010-12305-901 | |||||
| NTproBNP | EPX010-12251-901 | |||||
| Oncostatin M (OSM) | EPX01A-12163-901 | |||||
| Osteoactivin | EPX010-12449-901 | |||||
| Osteopontin | EPX01A-12066-901 | |||||
| Osteoprotegerin (OPG) | EPX01A-12021-901 | |||||
| PAI-1 (Serpin) | EPX01A-12033-901 | EPX01A-42033-901 | ||||
| PD-1 (CD279) | EPX01A-12214-901 | EPX010-26057-901 | ||||
| PDGF-BB | EPX01A-12071-901 | EPX01A-42071-901 | ||||
| PD-L1 (CD274) | EPX01A-12212-901 | EPX010-26054-901 | ||||
| PD-L2 | EPX01A-12215-901 | EPX010-26055-901 | ||||
| PECAM-1 | EPX01A-10229-901 | |||||
| Perforin | EPX010-12306-901 | |||||
| PIGF | EPX01A-12155-901 | |||||
| PTX3 | EPX010-12281-901 | |||||
| PVR | EPX010-12307-901 | |||||
| PYY | EPX010-12198-901 |
| ProcartaPlex analytes (R-S) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| RAGE | EPX010-12290-901 | |||||
| RANKL | EPX01A-12005-901 | EPX01A-26037-901 | EPX01A-32005-901 | |||
| RANTES (CCL5) | EPX01A-10287-901 | EPX01A-26009-901 | EPX01A-36009-901 | EPX01A-40287-901 | ||
| RBP4 | EPX01A-12205-901 | |||||
| REG3a | EPX010-12249-901 | |||||
| Renin | EPX010-12447-901 | |||||
| Resistin (ADSF) | EPX01A-12040-901 | EPX01A-42040-901 | ||||
| S100A8/A9 | EPX010-12334-901 | |||||
| S100B | EPX010-12339-901 | |||||
| SAA | EPX01A-12136-901 | |||||
| SAP (Pentraxin 2) | EPX01A-12156-901 | |||||
| SCF | EPX01A-12137-901 | EPX01A-42137-901 | EPX01B-50508-901 | |||
| SCGF-beta | EPX01A-12157-901 | |||||
| SDF-1 alpha (CXCL12a) | EPX01A-12138-901 | EPX01A-42138-901 | ||||
| Siglec-7 | EPX010-12308-901 | |||||
| Siglec-9 | EPX010-12309-901 | |||||
| ST2 (IL-33R) | EPX010-12231-901 | EPX01A-26047-901 | ||||
| Survivin (BIRC5) | EPX01A-12158-901 | |||||
| SYB16 (CXCL16) | EPX-010-26070-91 | |||||
| Syndecan-1 | EPX010-12233-901 |
| ProcartaPlex analytes (U-Z) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| UCHL1 | EPX010-12338-901 | |||||
| ULBP-1 | EPX010-12310-901 | |||||
| ULBP-3 | EPX010-12312-901 | |||||
| ULBP-4 | EPX010-12313-901 | |||||
| uPAR | EPX010-12294-901 | |||||
| Uromodulim | EPX010-12451-901 | |||||
| VCAM-1 | EPX01A-10232-901 | EPX01A-36046-901 | EPX01A-40232-901 | |||
| VEGF-A | EPX01A-10277-901 | EPX01A-20619-901 | EPX01A-30619-901 | EPX01A-40277-901 | EPX01A-50277-901 | |
| VEGF-D | EPX01A-12076-901 | EPX01A-42076-901 | ||||
| VEGF-R1 | EPX01A-10268-901 | |||||
| VEGF-R2 | EPX01A-12019-901 | EPX010-26067-901 | ||||
| VEGF-R3 | EPX01A-12064-901 | |||||
| VISTA (B7-H5) | EPX010-12327-901 | |||||
| ZAG | EPX01A-12201-901 |
High sensitivity Simplex kits
Invitrogen ProcartaPlex high sensitivity Simplex Kits are designed to measure small concentration differences in cell culture supernatants, plasma, and serum samples with 10-fold lower LLOQs (Lower Limit of Quantification) with sensitivities for all analytes in the femtogram range. These Simplex kits help overcome the sensitivity detection limits of conventional multiplex immunoassays.
ProcartaPlex high sensitivity Simplex kits are magnetic bead sets used to detect individual analytes. Create your own panel by combining multiple high sensitivity Simplex kits. A corresponding high sensitivity basic kit is required when combining simplex kits separate from any multiplex panel.
Human high sensitivity Simplex kits
| Analyte | Simplex Cat. No. | ULOQ* (pg/mL) | LLOQ* (pg/mL) | Inter Assay %CV | Intra Assay %CV |
|---|---|---|---|---|---|
| GM-CSF | EPXS010-10283-901 | 7,330 | 1.80 | <10 | <10 |
| IFN gamma | EPXS010-10228-901 | 260 | 0.31 | <10 | <10 |
| IL-1 beta | EPXS010-10224-901 | 1,330 | 0.32 | <10 | <10 |
| IL-2 | EPXS010-10221-901 | 490 | 0.44 | <10 | <10 |
| IL-4 | EPXS010-10225-901 | 560 | 0.86 | <10 | <10 |
| IL-5 | EPXS010-10278-901 | 2,720 | 0.66 | <10 | <10 |
| IL-6 | EPXS010-10213-901 | 2,850 | 1.03 | <10 | <10 |
| IL-8 | EPXS010-10204-901 | 890 | 0.22 | <10 | <10 |
| IL-10 | EPXS010-10215-901 | 750 | 0.18 | <10 | <10 |
| IL-12p70 | EPXS010-10238-901 | 3,090 | 0.75 | <10 | <10 |
| IL-17A | EPXS010-12017-901 | 1,030 | 0.25 | <10 | <10 |
| MCP-1 | EPXS010-10281-901 | 650 | 0.16 | <10 | <10 |
| TNF alpha | EPXS010-10223-901 | 1,390 | 0.45 | <10 | <10 |
Mouse high sensitivity Simplex kits
| Analyte | Simplex Cat. No. | ULOQ* (pg/mL) | LLOQ* (pg/mL) | Inter Assay %CV | Intra Assay %CV |
|---|---|---|---|---|---|
| IFN gamma | EPXS010-20606-901 | 1,250 | 0.31 | <10 | <10 |
| IL-2 | EPXS010-20601-901 | 1,820 | 0.44 | <10 | <10 |
| IL-4 | EPXS010-20613-901 | 3,530 | 0.86 | <10 | <10 |
| IL-6 | EPXS010-20603-901 | 4,230 | 1.03 | <10 | <10 |
| TNF alpha | EPXS010-20607-901 | 1,830 | 0.45 | <10 | <10 |
*determined for first lot
Quality control standards for ProcartaPlex Simplex Kits
| Specification | Description |
|---|---|
| Specificity | Proof of endogenous, native protein detection in specific samples |
| Sensitivity | Detection of relevant native protein levels |
| Precision | Inter-assay CV: <15%, Intra-assay CV: <15% |
| Linearity of Dilution | Linear results over the range of the assay |
| Flexibility | More than 90% of ProcartaPlex targets can be combined with one another. |
| Lot-to-lot consistency | Each new batch is calibrated against the reference standard lot. |
| Dynamic range | Large dynamic range of all ProcartaPlex assays |
Example data generated in the development process for a ProcartaPlex Simplex kit
| PD-L2 in standard ProcartaPlex Simplex format | Serum | EDTA Plasma | Citrate Plasma | Heparin Plasma |
|---|---|---|---|---|
| No. of Samples | 40 | 40 | 40 | 40 |
| Mean | 5,649.5 | 2,582.6 | 3,778.3 | 4,897.5 |
| Min. | 348.6 | 622.4 | 882.4 | 1,216.8 |
| Max. | 8,735.2 | 5,432.3 | 7,430.0 | 8,896.6 |
| SD | 2,065.9 | 1,302.9 | 1,509.9 | 1,980.6 |
| No. of positive samples | 40 | 40 | 40 | 40 |
Sensitivity: Detection of relevant native protein levels for typical sample types.
ProcartaPlex basic kits
To run simplex kits alone or in combination, the corresponding sample-specific basic kit is required. These basic kits contain all necessary support reagents to perform the assay.
| Basic Kit | Catalog No. |
|---|---|
| ProcartaPlex Human Basic Kit | EPX010-10420-901 |
| ProcartaPlex Mouse Basic Kit | EPX010-20440-901 |
| ProcartaPlex NHP Basic Kit | EPX010-40420-901 |
| ProcartaPlex Rat Basic Kit | EPX010-30420-901 |
| ProcartaPlex Porcine Basic Kit | EPX010-60460-901 |
| ProcartaPlex Canine Basic Kit | EPX010-50515-901 |
| ProcartaPlex Human High Sensitivity Basic Kit | EPXS010-10420-901 |
| ProcartaPlex Mouse High Sensitivity Basic Kit | EPXS010-20440-901 |
Additional accessories
ProcartaPlex supplemental products
| Product | Catalog No. |
|---|---|
| Hand-Held Magnetic Plate Washer | EPX-55555-000 |
| ProcartaPlex 96 well Flat Bottom Plate, black | EPX-44444-000 |
| Universal Assay Buffer (1X) | EPX-11111-000 |
| Universal Assay Buffer (10X) | EPX-11110-000 |
| Human Detection Antibody Diluent | EPX-33333-000 |
| Mouse Detection Antibody Diluent | EPX-22222-000 |
| ProcartaPlex Streptavidin-PE | EPX-SAPE-000 |
| ProcartaPlex Wash Buffer 10 ml (10X) | EPX-66666-000 |
| ProcartaPlex Wash Buffer 200 ml (10X) | EPX-66666-001 |
| ProcartaPlex Reading Buffer | EPX-77777-000 |
| ProcartaPlex Cell Lysis Buffer | EPX-99999-000 |
| ProcartaPlex Cell Lysate Assay Diluent (1X) | EPX-11114-000 |
| ProcartaPlex Platinum Assay Buffer for Plasma Samples | EPXP-11112-000 |
| ProcartaPlex Platinum Assay Buffer for Serum Samples | EPXP-11113-000 |
ProcartaPlex protocols
Additional protocols
ProcartaPlex Product information
Find your target analyte
| ProcartaPlex analytes (A) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| 4-1BB (CD137) | EPX01A-10289-901 | |||||
| 4-1BBL (CD137L) | EPX010-15901-901 | EPX010-26058-901 | ||||
| 6Ckine (CCL21) | EPX010-12384-901 | |||||
| Adiponectin | EPX01A-12032-901 | EPX01A-26038-901 | EPX01A-42032-901 | |||
| Alpha-1-Microglobulin | EPX010-12452-901 | |||||
| Alpha-synuclein [pS129] | EPX010-12361-901 | |||||
| Alpha-synuclein (total) | EPX010-12360-901 | |||||
| Amyloid beta (1–40) | EPX010-12350-901 | |||||
| Amyloid beta (1–42) | EPX010-12351-901 | |||||
| Angiogenin | EPX010-12234-901 | |||||
| Angiopoietin-1 | EPX010-12235-901 | |||||
| Angiostatin | EPX010-12237-901 | |||||
| Apolipoprotein E4 | EPX010-12344-901 | |||||
| APRIL | EPX01A-12008-901 | |||||
| Arginase-1 | EPX010-12216-901 | |||||
| AXL | EPX010-12286-901 |
| ProcartaPlex analytes (B) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| B7-H6 | EPX010-12328-901 | |||||
| BACE1 (beta-secretase 1) | EPX010-12358-901 | |||||
| BAFF | EPX01A-12007-901 | EPX01A-26096-901 | ||||
| BDNF | EPX01A-12116-901 | EPX01A-42116-901 | ||||
| Beta-2-microglobulin (B2M) | EPX010-12414-901 | |||||
| Betacellulin (BTC) | EPX01A-26042-901 | |||||
| Big ET-1 | EPX010-12266-901 | |||||
| BLC (CXCL13) | EPX01A-12147-901 | EPX01A-42147-901 | ||||
| BMP-2 | EPX010-12319-901 | |||||
| BMP-9 | EPX010-12246-901 | |||||
| BTLA (CD272) | EPX01A-12217-901 | EPX010-26052-901 |
| ProcartaPlex analytes (D-F) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| D-Dimer | EPX01A-12149-901 | |||||
| DR6 | EPX01A-15904-901 | |||||
| E-cadherin | EPX010-12315-901 | |||||
| EDA-1 | EPX01A-15903-901 | |||||
| EGF | EPX01A-12070-901 | |||||
| Elafin | EPX010-12248-901 | |||||
| EMMPRIN | EPX010-12242-901 | |||||
| ENA-78 (CXCL5) | EPX01A-12119-901 | EPX01A-26043-901 | ||||
| Endoglin (CD105) | EPX01A-12105-901 | |||||
| Endostatin | EPX010-12238-901 | |||||
| Eotaxin (CCL11) | EPX01B-12120-901 | EPX01A-26008-901 | EPX01A-36008-901 | EPX01B-42120-901 | ||
| Eotaxin-2 (CCL24) | ||||||
| Eotaxin-3 (CCL26) | EPX01A-12140-901 | |||||
| FABP-3 | EPX010-12263-901 | |||||
| Factor X | EPX01A-12152-901 | |||||
| Fas (APO) | EPX01A-10245-901 | |||||
| Fas-L | EPX01A-10260-901 | |||||
| Ferritin | EPX010-12184-901 | |||||
| Fetuin-A | EPX010-12347-901 | |||||
| FGF-2 | EPX01A-12074-901 | EPX01A-42074-901 | ||||
| FGF-21 | EPX010-12357-901 | |||||
| FGF-23 | EPX010-12204-901 | |||||
| Fibrinogen | EPX01A-12153-901 | |||||
| Flt3-ligand | EPX010-12387-901 | |||||
| Follistatin | EPX010-12244-901 | |||||
| Fractalkine (CX3CL1) | EPX01A-12121-901 |
| ProcartaPlex analytes (G-H) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| Galectin-3 | EPX01A-10279-901 | |||||
| Galectin-9 | EPX010-12331-901 | |||||
| GAPDH | EPX01A-12154-901 | |||||
| GAS6 | EPX010-12291-901 | |||||
| GCP-2 (CXCL6) | EPX010-12386-901 | |||||
| G-CSF (CSF-3) | EPX01A-12001-901 | EPX01A-26034-901 | EPX01A-36034-901 | EPX01A-42001-901 | ||
| GDF-15 | EPX010-12258-901 | |||||
| GDNF | EPX010-12340-901 | |||||
| GFAP | EPX010-12336-901 | |||||
| Ghrelin | EPX010-12192-901 | |||||
| GITR (CD357) | EPX01A-12210-901 | |||||
| GITRL | EPX01A-15902-901 | |||||
| GLP-1 | EPX010-12194-901 | |||||
| GM-CSF | EPX01A-10283-901 | EPX01A-20612-901 | EPX01A-30612-901 | EPX01A-40283-901 | ||
| gp130 (IL-6RB) | EPX010-12376-901 | |||||
| Granzyme A | EPX010-12232-901 | |||||
| Granzyme B | EPX01A-12027-901 | EPX01A-42027-901 | ||||
| GRO alpha (KC/CXCL1) | EPX01A-12122-901 | EPX01A-26031-901 | EPX01A-36031-901 | |||
| GSTA1 (Glutathione S-transferase A1) | EPX010-12450-901 | |||||
| HB-EGF | EPX010-12245-901 | |||||
| HER-2 | EPX01A-10207-901 | |||||
| HGF | EPX01A-12069-901 | |||||
| HSP60 | EPX010-12323-901 | |||||
| HVEM (CD270) | EPX01A-12218-901 |
| ProcartaPlex analytes (K-L) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| Kallikrein-6 (KLK6) | EPX010-12355-901 | |||||
| KIM-1 (TIM-e/HAVCR1) | EPX010-12445-901 | |||||
| KRT18 | EPX010-12247-901 | |||||
| Lactoferrin | EPX010-12250-901 | |||||
| LAG-3 | EPX01A-12211-901 | EPX010-26051-901 | ||||
| LAP (TGF-beta 1) | EPX010-12065-901 | |||||
| LDH-B | EPX010-12262-901 | |||||
| Leptin | EPX01A-12039-901 | EPX01A-26036-901 | EPX01A-32039-901 | |||
| LIF | EPX01A-10242-901 | EPX01A-26040-901 | ||||
| LIGHT | EPX01A-12009-901 | |||||
| LOX-1 | EPX010-12296-901 | |||||
| Lp-PLA2 | EPX010-12324-901 | |||||
| LYVE-1 | EPX010-12239-901 |
| ProcartaPlex analytes (N-P) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| NCAM-1 | EPX010-12354-901 | |||||
| Nectin-2 | EPX010-12304-901 | |||||
| Neurogranin (NRGN) | EPX010-12352-901 | |||||
| NF-H | EPX010-12337-901 | |||||
| NGAL | EPX01A-12202-901 | |||||
| NGF-beta | EPX01A-12117-901 | EPX01A-32117-901 | EPX01A-42117-901 | EPX01A-52117-901 | ||
| NRP-1 | EPX010-12243-901 | |||||
| NSE | EPX010-12335-901 | |||||
| NT5E | EPX010-12305-901 | |||||
| NTproBNP | EPX010-12251-901 | |||||
| Oncostatin M (OSM) | EPX01A-12163-901 | |||||
| Osteoactivin | EPX010-12449-901 | |||||
| Osteopontin | EPX01A-12066-901 | |||||
| Osteoprotegerin (OPG) | EPX01A-12021-901 | |||||
| PAI-1 (Serpin) | EPX01A-12033-901 | EPX01A-42033-901 | ||||
| PD-1 (CD279) | EPX01A-12214-901 | EPX010-26057-901 | ||||
| PDGF-BB | EPX01A-12071-901 | EPX01A-42071-901 | ||||
| PD-L1 (CD274) | EPX01A-12212-901 | EPX010-26054-901 | ||||
| PD-L2 | EPX01A-12215-901 | EPX010-26055-901 | ||||
| PECAM-1 | EPX01A-10229-901 | |||||
| Perforin | EPX010-12306-901 | |||||
| PIGF | EPX01A-12155-901 | |||||
| PTX3 | EPX010-12281-901 | |||||
| PVR | EPX010-12307-901 | |||||
| PYY | EPX010-12198-901 |
| ProcartaPlex analytes (R-S) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| RAGE | EPX010-12290-901 | |||||
| RANKL | EPX01A-12005-901 | EPX01A-26037-901 | EPX01A-32005-901 | |||
| RANTES (CCL5) | EPX01A-10287-901 | EPX01A-26009-901 | EPX01A-36009-901 | EPX01A-40287-901 | ||
| RBP4 | EPX01A-12205-901 | |||||
| REG3a | EPX010-12249-901 | |||||
| Renin | EPX010-12447-901 | |||||
| Resistin (ADSF) | EPX01A-12040-901 | EPX01A-42040-901 | ||||
| S100A8/A9 | EPX010-12334-901 | |||||
| S100B | EPX010-12339-901 | |||||
| SAA | EPX01A-12136-901 | |||||
| SAP (Pentraxin 2) | EPX01A-12156-901 | |||||
| SCF | EPX01A-12137-901 | EPX01A-42137-901 | EPX01B-50508-901 | |||
| SCGF-beta | EPX01A-12157-901 | |||||
| SDF-1 alpha (CXCL12a) | EPX01A-12138-901 | EPX01A-42138-901 | ||||
| Siglec-7 | EPX010-12308-901 | |||||
| Siglec-9 | EPX010-12309-901 | |||||
| ST2 (IL-33R) | EPX010-12231-901 | EPX01A-26047-901 | ||||
| Survivin (BIRC5) | EPX01A-12158-901 | |||||
| SYB16 (CXCL16) | EPX-010-26070-91 | |||||
| Syndecan-1 | EPX010-12233-901 |
| ProcartaPlex analytes (U-Z) | Human | Mouse | Rat | NHP | Canine | Porcine |
|---|---|---|---|---|---|---|
| UCHL1 | EPX010-12338-901 | |||||
| ULBP-1 | EPX010-12310-901 | |||||
| ULBP-3 | EPX010-12312-901 | |||||
| ULBP-4 | EPX010-12313-901 | |||||
| uPAR | EPX010-12294-901 | |||||
| Uromodulim | EPX010-12451-901 | |||||
| VCAM-1 | EPX01A-10232-901 | EPX01A-36046-901 | EPX01A-40232-901 | |||
| VEGF-A | EPX01A-10277-901 | EPX01A-20619-901 | EPX01A-30619-901 | EPX01A-40277-901 | EPX01A-50277-901 | |
| VEGF-D | EPX01A-12076-901 | EPX01A-42076-901 | ||||
| VEGF-R1 | EPX01A-10268-901 | |||||
| VEGF-R2 | EPX01A-12019-901 | EPX010-26067-901 | ||||
| VEGF-R3 | EPX01A-12064-901 | |||||
| VISTA (B7-H5) | EPX010-12327-901 | |||||
| ZAG | EPX01A-12201-901 |
High sensitivity Simplex kits
Invitrogen ProcartaPlex high sensitivity Simplex Kits are designed to measure small concentration differences in cell culture supernatants, plasma, and serum samples with 10-fold lower LLOQs (Lower Limit of Quantification) with sensitivities for all analytes in the femtogram range. These Simplex kits help overcome the sensitivity detection limits of conventional multiplex immunoassays.
ProcartaPlex high sensitivity Simplex kits are magnetic bead sets used to detect individual analytes. Create your own panel by combining multiple high sensitivity Simplex kits. A corresponding high sensitivity basic kit is required when combining simplex kits separate from any multiplex panel.
Human high sensitivity Simplex kits
| Analyte | Simplex Cat. No. | ULOQ* (pg/mL) | LLOQ* (pg/mL) | Inter Assay %CV | Intra Assay %CV |
|---|---|---|---|---|---|
| GM-CSF | EPXS010-10283-901 | 7,330 | 1.80 | <10 | <10 |
| IFN gamma | EPXS010-10228-901 | 260 | 0.31 | <10 | <10 |
| IL-1 beta | EPXS010-10224-901 | 1,330 | 0.32 | <10 | <10 |
| IL-2 | EPXS010-10221-901 | 490 | 0.44 | <10 | <10 |
| IL-4 | EPXS010-10225-901 | 560 | 0.86 | <10 | <10 |
| IL-5 | EPXS010-10278-901 | 2,720 | 0.66 | <10 | <10 |
| IL-6 | EPXS010-10213-901 | 2,850 | 1.03 | <10 | <10 |
| IL-8 | EPXS010-10204-901 | 890 | 0.22 | <10 | <10 |
| IL-10 | EPXS010-10215-901 | 750 | 0.18 | <10 | <10 |
| IL-12p70 | EPXS010-10238-901 | 3,090 | 0.75 | <10 | <10 |
| IL-17A | EPXS010-12017-901 | 1,030 | 0.25 | <10 | <10 |
| MCP-1 | EPXS010-10281-901 | 650 | 0.16 | <10 | <10 |
| TNF alpha | EPXS010-10223-901 | 1,390 | 0.45 | <10 | <10 |
Mouse high sensitivity Simplex kits
| Analyte | Simplex Cat. No. | ULOQ* (pg/mL) | LLOQ* (pg/mL) | Inter Assay %CV | Intra Assay %CV |
|---|---|---|---|---|---|
| IFN gamma | EPXS010-20606-901 | 1,250 | 0.31 | <10 | <10 |
| IL-2 | EPXS010-20601-901 | 1,820 | 0.44 | <10 | <10 |
| IL-4 | EPXS010-20613-901 | 3,530 | 0.86 | <10 | <10 |
| IL-6 | EPXS010-20603-901 | 4,230 | 1.03 | <10 | <10 |
| TNF alpha | EPXS010-20607-901 | 1,830 | 0.45 | <10 | <10 |
*determined for first lot
Quality control standards for ProcartaPlex Simplex Kits
| Specification | Description |
|---|---|
| Specificity | Proof of endogenous, native protein detection in specific samples |
| Sensitivity | Detection of relevant native protein levels |
| Precision | Inter-assay CV: <15%, Intra-assay CV: <15% |
| Linearity of Dilution | Linear results over the range of the assay |
| Flexibility | More than 90% of ProcartaPlex targets can be combined with one another. |
| Lot-to-lot consistency | Each new batch is calibrated against the reference standard lot. |
| Dynamic range | Large dynamic range of all ProcartaPlex assays |
Example data generated in the development process for a ProcartaPlex Simplex kit
| PD-L2 in standard ProcartaPlex Simplex format | Serum | EDTA Plasma | Citrate Plasma | Heparin Plasma |
|---|---|---|---|---|
| No. of Samples | 40 | 40 | 40 | 40 |
| Mean | 5,649.5 | 2,582.6 | 3,778.3 | 4,897.5 |
| Min. | 348.6 | 622.4 | 882.4 | 1,216.8 |
| Max. | 8,735.2 | 5,432.3 | 7,430.0 | 8,896.6 |
| SD | 2,065.9 | 1,302.9 | 1,509.9 | 1,980.6 |
| No. of positive samples | 40 | 40 | 40 | 40 |
Sensitivity: Detection of relevant native protein levels for typical sample types.
ProcartaPlex basic kits
To run simplex kits alone or in combination, the corresponding sample-specific basic kit is required. These basic kits contain all necessary support reagents to perform the assay.
| Basic Kit | Catalog No. |
|---|---|
| ProcartaPlex Human Basic Kit | EPX010-10420-901 |
| ProcartaPlex Mouse Basic Kit | EPX010-20440-901 |
| ProcartaPlex NHP Basic Kit | EPX010-40420-901 |
| ProcartaPlex Rat Basic Kit | EPX010-30420-901 |
| ProcartaPlex Porcine Basic Kit | EPX010-60460-901 |
| ProcartaPlex Canine Basic Kit | EPX010-50515-901 |
| ProcartaPlex Human High Sensitivity Basic Kit | EPXS010-10420-901 |
| ProcartaPlex Mouse High Sensitivity Basic Kit | EPXS010-20440-901 |
Additional accessories
ProcartaPlex supplemental products
| Product | Catalog No. |
|---|---|
| Hand-Held Magnetic Plate Washer | EPX-55555-000 |
| ProcartaPlex 96 well Flat Bottom Plate, black | EPX-44444-000 |
| Universal Assay Buffer (1X) | EPX-11111-000 |
| Universal Assay Buffer (10X) | EPX-11110-000 |
| Human Detection Antibody Diluent | EPX-33333-000 |
| Mouse Detection Antibody Diluent | EPX-22222-000 |
| ProcartaPlex Streptavidin-PE | EPX-SAPE-000 |
| ProcartaPlex Wash Buffer 10 ml (10X) | EPX-66666-000 |
| ProcartaPlex Wash Buffer 200 ml (10X) | EPX-66666-001 |
| ProcartaPlex Reading Buffer | EPX-77777-000 |
| ProcartaPlex Cell Lysis Buffer | EPX-99999-000 |
| ProcartaPlex Cell Lysate Assay Diluent (1X) | EPX-11114-000 |
| ProcartaPlex Platinum Assay Buffer for Plasma Samples | EPXP-11112-000 |
| ProcartaPlex Platinum Assay Buffer for Serum Samples | EPXP-11113-000 |
ProcartaPlex protocols
Additional protocols
ProcartaPlex Product information
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Performance guarantee
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Learn more about performance guarantee
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