Try our oligo calculator to determine volumes needed to resuspend your DNA oligos to desired concentrations, estimate the percentage of full-length product for different oligo lengths, and calculate final yield based on oligo length, synthesis scale, and purification method.
Resuspension calculator
This calculates the volume needed to resuspend an oligo, given that nmoles and desired concentration are known.
| Term | Number |
|---|---|
| nmol Oligo Yield (from COA) | |
| Desired conc. (µM or µmol/L) | |
| Volume Required (µL) | ... |
Estimated percentage of full length product calculator
This calculates the estimated percentage of full length product based on the oligo's length.
| Term | Number |
|---|---|
| Oligo length (nt) | |
| Chem. reaction efficiency (typically 98%-99% efficient) | 99% |
| % Full length product | ... |
Minimum yield guarantee oligo calculator
This calculates the minimum yield guaranteed* based on the oligo length (nt) and synthesis scale.
| Term | Number |
|---|---|
| Oligo length (nt) | |
| Approx. mol. weight (daltons or g/mol) | ... |
| pmol in 1 µg of primer | ... |
Minimum amount (nmoles) delivered
The amounts calculated in the table below represent the minimum guaranteed amount of oligo you will receive based on the oligo length (nt) and synthesis scale.
| Synthesis scales | Desalted (nmol) | Cartridge (nmol) | HPLC (nmol) | PAGE (nmol) |
|---|---|---|---|---|
| 25 nmol | N/A | N/A | N/A | |
| 50 nmol | ||||
| 200 nmol | ||||
| 1000 nmol (1 µmol) | ||||
| 10000 nmol (10 µmol) | N/A |
Minimum amount (µL) delivered
The volumes calculated in the table below represent the minimum guaranteed amount of oligo you will receive based on the oligo length (nt), synthesis scale, and selected concentration.
| Selected conc. | Synthesis scales | Desalted (µL) | Cartridge (µL) | HPLC (µL) | PAGE (µL) |
|---|---|---|---|---|---|
| 10 µM | 25 nmol | N/A | N/A | N/A | |
| 50 nmol | |||||
| 200 nmol | |||||
| 1 µmol | |||||
| 10 µmol | N/A | ||||
| 20 µM | 25 nmol | N/A | N/A | N/A | |
| 50 nmol | |||||
| 200 nmol | |||||
| 1 µmol | |||||
| 10 µmol | N/A | ||||
| 50 µM | 25 nmol | N/A | N/A | N/A | |
| 50 nmol | |||||
| 200 nmol | |||||
| 1 µmol | |||||
| 10 µmol | N/A | ||||
| 100 µM | 25 nmol | N/A | N/A | N/A | |
| 50 nmol | |||||
| 200 nmol | |||||
| 1 µmol | |||||
| 10 µmol | N/A | ||||
| 200 µM | 25 nmol | N/A | N/A | N/A | |
| 50 nmol | |||||
| 200 nmol | |||||
| 1 µmol | |||||
| 10 µmol | NA |
* Minimum yields guaranteed – when ordering custom oligos, remember that the scale of synthesis (25 nmol, 50 nmol, 200 nmol, 1 µmol, or 10 µmol) is the starting point for synthesis, not the guaranteed final amount. Oligonucleotide length, sequence, GC content, and coupling efficiencies can vary the yield of nmole amounts post-synthesis. Invitrogen guarantees the total yield of oligonucleotide as a minimum number of OD units. Purification by HPLC, PAGE, and Cartridge will also affect final product yield.
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For Research Use Only. Not for use in diagnostic procedures.