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Switching from 293 to CHO cells during drug development doesn't have to create uncertainty. Now there's a better way. The Gibco ExpiCHO Expression System is a completely optimized system consisting of ExpiCHO-S(TM) cells that have been adapted to high-density, serum-free suspension culture in ExpiCHO(TM) Expression Medium, along with specially designed transfection reagents and enhancers, that provide the highest yields possible in a transient system. That means you can start your research work in CHO cells and stay in CHO cells through discovery.
“The ExpiCHO system helps speed up our developmental timelines by eliminating the need to have different hosts for transient versus stable expression. This eliminates a key quality risk when we transition from research into development.” Biotechnology company
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Review these videos to learn how to produce recombinant proteins using the ExpiCHO Expression System and also to hear about the latest advances in protein expression from industry experts.
The Gibco ExpiCHO Expression System brings together a high-expressing CHO cell line, a chemically-defined animal origin-free, serum-free, and protein-free culture medium, an optimized culture feed, and a high-efficiency transfection reagent that work in concert to provide protein titers 3x higher than the Gibco Expi293 Expression System and 160x higher than the Gibco FreeStyle CHO Expression System. With the chemically-defined formulation of the ExpiCHO(TM) Expression Medium, you can come to expect high reproducibility in your results and lot-to-lot reliability.
The ExpiCHO Expression System is designed to deliver superior protein yields compared to alternative transient systems, saving you precious time, incubator space, and plasticware costs. Now you can express the same amount of protein in a single flask that other transient CHO systems express in 20 or more flasks.
“The higher titers achieved by the ExpiCHO system… saves us considerable time, effort, and money." Bonnie Hammer, PhD, Vice President of Biologics Development, Invenra
Figure 1. Recombinant protein titers in ExpiCHO and other transient CHO expression systems. Expression levels of human IgG, rabbit IgG and erythropoietin in ExpiCHO and other transient CHO expression systems are shown. ExpiCHO titers range from 25–160 times those of other transient CHO expression systems.
Expression levels of human IgG, rabbit IgG, murine IgG, and a broad range of non-Ab proteins in Expi293 or other transient expression systems and ExpiCHO systems are shown. IgG titers in ExpiCHO Expression System range from 0.6–97–fold (Avg. 5-fold) than those obtained using various other systems. Additionally, 7 proteins from this panel were not expressed in Expi293 cells yet expressed at high levels in the ExpiCHO system.
| Protein | Expi293 or current system (mg/L) | ExpiCHO (mg/L) | Fold increase |
|---|---|---|---|
| Human antibody | 26.6 | 2,580 | 97 |
| 4 | 147 | 33 | |
| 4 | 56 | 14.6 | |
| 22 | 266 | 12.4 | |
| 48 | 334 | 6.9 | |
| 36 | 190 | 5.3 | |
| 18 | 78 | 4.3 | |
| 85 | 314 | 3.7 | |
| 170 | 410 | 2.4 | |
| 138 | 325 | 2.4 | |
| 25 | 50 | 2.0 | |
| 102 | 204 | 2.0 | |
| 57 | 92 | 1.6 | |
| 336 | 491 | 1.5 | |
| 565 | 864 | 1.5 | |
| 450 | 600 | 1.3 | |
| 350 | 450 | 1.3 | |
| 309 | 383 | 1.2 | |
| 243 | 276 | 1.1 | |
| 352 | 371 | 1.1 | |
| 565 | 620 | 1.1 | |
| 309 | 302 | 1.0 | |
| 139 | 129 | 0.9 | |
| 11 | 9 | 0.8 | |
| 565 | 365 | 0.6 | |
| Undetectable | 500 | ||
| Precipitated | 330 |
| Protein | Expi293 or current system (mg/L) | ExpiCHO (mg/L) | Fold increase |
|---|---|---|---|
| Rabbit antibody | 5 | 120 | 20 |
| 17 | 178 | 11 | |
| Murine antibody | 216 | 1,412 | 6.5 |
| 20 | 86 | 4.3 | |
| 60 | 240 | 4.0 | |
| 60 | 240 | 4.0 | |
| 76 | 196 | 2.6 | |
| 12 | 18 | 1.5 | |
| 16 | 17 | 1.1 | |
| 119 | 48 | 0.4 | |
| 135 | 47 | 0.3 |
| Protein | Expi293 or current system (mg/L) | ExpiCHO (mg/L) | Fold increase |
|---|---|---|---|
| Bi-TE | Western blot | Western blot | 25 |
| Bi-specific | 28 | 117 | 4.2 |
| 26 | 90 | 3.5 | |
| 126 | 250 | 2.0 | |
| NA | 763 | ||
| NA | 660 | ||
| Secreted | 216 | 1412 | 6.5 |
| Protein | Expi293 or current system (mg/L) | ExpiCHO (mg/L) | Fold increase |
|---|---|---|---|
| Non-Ab | 15 | 82 | 5.5 |
| Western blot | Western blot | 5.0 | |
| 164 | 68 | 0.4 | |
| NA | 206 | ||
| Fab | 85 | 366 | 4.3 |
| 10 | 10 | 1.0 | |
| FC-fusion | 5 | 8 | 1.7 |
| His Tag HLA | Undetectable | 12 | 12 |
| Viral protein | Western blot | Western blot | 3–5 |
| Cytokine | Western blot | Western blot | 5–10 |
| FcR | Western blot | Western blot | 2–3 |
| ExpiCHO Expression System | Expi293 Expression System | FreeStyle CHO Expression System | PEI MAX + TransFx-C | MaxCyte + TransFx-C | |
|---|---|---|---|---|---|
| Cost of 1L transfection | $2,174 | $1,735 | $1,201 | $544 | $2,999 |
| Culture volume for equivalent yield | 70 mL | 100 mL | 2,000 mL | 1,500 mL | 100 mL |
| Total cost for same amount of protein | $146 | $174 | $2,403 | $2,403 | $997* |
| *Factors in minimum cost of a MaxCyte processing assembly and a portion of the upfront cost of purchasing a MaxCyte electroporation instrument. NOTE: Cost comparison based on list price in USD and protein yields of 1.5 g/L with ExpiCHO Expression System, 1 g/L with Expi293 Expression System, and 0.05 g/L with FreeStyle CHO Expression System. | |||||
The ExpiCHO Expression System provides multiple protocols to fit your workflow. The system can scale up or down based on your needs for greater throughput early in discovery or higher yields as you focus on selected proteins.
“The ExpiCHO system is so easy it takes less work than making proteins in E. coli. The scalability of the system is fantastic. I can express 30 different mAbs simultaneously, and then pick the ones I like and scale up to grams. The ExpiCHO system is a revolutionary lifesaver for us.” Arjen van den Berg, PhD, Senior Scientist, Cellular and Molecular Medicine, University of California at San Diego
Figure 2. Scalability of the ExpiCHO System. The ExpiCHO System is scalable within 15% of control from 35 mL to 1 L culture volumes.
Figure 3. Transient transfection protocol—7 or 8 steps to protein production. The ExpiCHO system allows you to customize your work with a choice of 3 different protocols depending on your unique research needs, time, and equipment availability.
Figure 4. Equivalent rabbit IgG titers are obtained in cGMP-banked ExpiCHO-S cells and non-cGMP ExpiCHO-S cells at 30 mL scale using ExpiCHO-S Expression System.
Gibco ExpiCHO Stable Production Medium (SPM) is specifically designed to simplify your cell line development process, facilitating the production stage with minimal effort and a high degree of confidence. This chemically defined, protein-free, animal origin component-free formulation is fully optimized for large-scale fed-batch culture with ExpiCHO-S cells. ExpiCHO SPM supports transition from transient to stable production and offers high titers typically without the need for additional medium optimization.
Sterile, single-use Thermo Scientific Nalgene flasks are made from light, break-resistant, crystal-clear PETG, so you can express with confidence. All sizes are available in vented or non-vented formats, and with plain or baffled bottoms for use on the benchtop or shaker table.
See protocol for flasks validated with ExpiCHO Expression system
We offer a full array of innovative products and services, including fast and flexible cell line development, media and feeds, class-setting technologies, quality custom media development, and media optimizing services.
Learn more about how we can customize your media formulation and format
Protein Expression Publication Hub
Access a curated list of publications that highlights the advantages of our Expi protein expression systems.
For Research Use Only. Not for use in diagnostic procedures.
