One Lambda HybriType NGS Assay

Hybrid Capture NGS genotyping


The One Lambda NGS hybrid capture assay utilizes a probe-based design to optimize read balance and uniformity, potentially preventing allele dropout for quality results.
 

Hybrid capture next-generation sequencing (NGS) is a targeted sequencing technique used to selectively enrich and sequence specific regions of the genome. It combines hybrid capture for target enrichment with NGS for sequencing, enabling detailed analysis of selected genomic regions.

 

Our simple workflow, product design and comprehensive gene coverage can potentially reduce ambiguity, provide balanced reads and reduce turnaround and hands on time.

 

Analysis using TypeStream Visual NGS Analysis Software delivers rapid, automated analysis while supporting genotyping and detection of gene copy number values across HLA and non-HLA genes.

Features and benefits

Simple workflow

  • Universal workflow for blood and buccal samples

  • Unique dual indexing 

  • ≤ 5.5 hours turnaround time

Modular content

  • 18 loci (2 panels) - 11 HLA & 7 extended MHC

  • ABO group

  • Extended coverage 

Quality results

  • Balanced reads1

  • High resolution

  • Copy number values

1 Hybrid capture enrichment avoids issues related to amplification bias.

How does it work?

The One Lambda HybriType assay offers a streamlined library prep method with fast turnaround time, low bias and minimal artifacts. The workflow may improve laboratory efficiency, with as little as 2.5 hours of hands-on time and next day results.
 

Fig. 1. Workflow.

Genomic library preparation

A streamlined library construction with:

  • Universal protocol for blood and buccal samples

  • Single step fragmentation, repair, and adenylation1 

  • Single step barcoding in a user-friendly plate format2 

  • UDI barcodes improve tracking and prevent index hopping 

  • No extra lab equipment needed

1 Provided in a ready-to-use master mix format.
2 No manual mixing of i7 + i5 barcodes is required.

Target enrichment

One Lambda HybriType assay offers shorter incubations, easier washes, and faster turnaround time:

  • Ready-to-use wash buffers require no pre-heating

  • Streamlined washing process

        Reduced wash steps

        Simple to follow

  • Less technique-sensitive

        Incubations at room temperature or in a thermal cycler

Modular content

One Lambda HybriType assay has been deigned to provide flexibility in testing configuration and coverage. This enables broad genetic coverage across diverse populations. Non-classical MHC Loci genotyping panel enables advanced compatibility research and extends testing beyond traditional HLA typing. ABO genotyping for blood groups and subtypes expands testing options with no additional workflows or techniques, providing greater confidence with results.

  • 18 loci (2 panel) configuration offers greater flexibility in sequencing 11 HLA and 7 extended MHC loci

  • ABO blood group panel designed to support evolving laboratory requirements

  • Extended coverage reduces ambiguity and minimizes reflex testing

One Lambda HybriType assay provides coverage across a wide range of loci with the capability to sequence additional genes with a single pipetting step.

11 Loci Panel

Target Coverage
HLA-A Full Gene
HLA-B Full Gene
HLA-C Full Gene
HLA-DBR1 Full Exon†
HLA-DRB345 Full Exon†
HLA-DQB1 Full Gene
HLA-DQA1 Full Gene
HLA-DPB1 Full Exon†
HLA-DPA1 Full Gene

7 Loci Extended Panel

Target Coverage
HLA-E Full Gene
HLA-F Full Gene
HLA-G Full Gene
HLA-H Full Gene
HLA-J Full Gene
MCA Full Gene
MICB Full Exon†

ABO Panel

Target Coverage
ABO Full Exon†

† Average >85% base coverage across intronic regions.

Quality results

One Lambda HybriType data supports Class I (4-Field) and Class II (3-Field) high-resolution genotyping analysis across various sample types, including whole blood and buccal swabs. Copy Number Values calculates the number of copies of each allele per locus to assess zygosity and improve confidence in results.

 

  • Balanced reads with hybrid capture enrichment minimizing PCR bias for uniform sequencing
  • High resolution data has the potential to reduce ambiguity and reflexive testing
  • Copy number values provide deeper insight into the relationship between allele balance and zygosity 

 

TypeStream Visual (TSV) analysis

Data for One Lambda HybriType assay is analyzed using our NGS software – TypeStream Visual providing automated and streamlined analysis with a wide range of analytical tools, run statistics and quality metrics.

Copy Number Variation (CNV) tool provides information on the number of copies of each allele per locus. TypeStream Visual analyzes read counts by comparing within and across samples:

  • Homozygous vs heterozygous
  • Homozygous vs hemizygous
  • Nullizygous results
  • Additional copies of genes (e.g. MICA)

 

The One Lambda HybriType assay is compatible with the Illumina™ platform, including the MiSeq™, MiniSeq™ and iSeq™ instruments.

 

 

For Research Use Only. Not for use in diagnostic procedures.