APC excitation shown as dotted line and emission shown as solid red histogram
5594-633660/20651660(in buffer) 3flow cytometry

Allophycocyanin (APC) is an intensely bright phycobiliprotein isolated from red algae that exhibits far-red fluorescence with high quantum yields. It is excited by laser lines at 594 and 633 nm, with an absorbance maximum at 650 nm and a fluorescence emission peak at 660 nm.

APC is a large chemically-crosslinked molecule used in fluorescence-based detection, primarily for flow cytometry, microarray assays, ELISAs, and other applications that require high sensitivity, but not photostability. We offer a broad range of APC products, including primary antibodies, secondary antibodies, streptavidin, and tandem conjugates. In practical applications, the sensitivity of APC conjugates is usually 5 to 10 times greater than that of the corresponding fluorescein conjugate.

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Experimental data using Allophycocyanin (APC)
2 peak histogram showing cells stained without (left) or with (right) CD4 APC
Figure 1. Flow cytometry histogram showing human mononuclear cells stained with CD4 APC.
left plot shows CD3 FITC positive and APC isotype control; right plot shows CD3 FITC and CD4 APC dual positive sample

Figure 2. Staining of mouse splenocytes with Anti-Mouse CD3 FITC and 0.06 μg of Rat IgG2a K Isotype Control APC (left) or 0.06 μg of Anti-Mouse CD4 APC (right). Cell in the lymphocyte gate were used for analysis.