BODIPY FL excitation shown as dotted line and emission shown as solid green histogram
3488FITC502511(in buffer) 3
(in antifade) 4		microscopy, flow cytometry, fluorescence microplate reader

Invitrogen BODIPY FL dye is a bright, green-fluorescent dye with excitation and emission similar to fluorescein (FITC) or Alexa Fluor 488 dye. It has a high extinction coefficient and fluorescence quantum yield and is relatively insensitive to solvent polarity and pH change. In contrast to the highly water-soluble fluorophores Alexa Fluor 488 dye and fluorescein (FITC), BODIPY dyes have unique hydrophobic properties excellent for staining lipids, membranes, and other lipophilic compounds.

BODIPY FL dye has a relatively long excited-state lifetime (typically 5 nanoseconds or longer), which is useful for fluorescence polarization–based assays and a large two-photon cross-section for multiphoton excitation.

We offer BODIPY FL dye conjugated to a variety of antibodies, peptides, proteins, tracers, and amplification substrates optimized for cellular labeling and detection. In addition, reactive dye forms and protein labeling kits are available to help you generate your own protein conjugates or probes.

Protein Labeling Reagents Selection Guide (NHS ester, maleimide, etc.)  See all BODIPY dyes

Additional products

Experimental data using BODIPY FL
Microscopic view of BPAEC stained with alpha-tubulin (green), F-actin (red), and nuclei (blue)
Fluorescence microscopic analysis of cellular components in BPAEC.FluoCells Prepared Slide #2, shows bovine pulmonary artery endothelial cells (BPAEC) stained with alpha-Tubulin Monoclonal Antibody in conjunction with Goat anti-Mouse IgG Cross-Adsorbed Secondary Antibody, BODIPY FL for labeling microtubules. Texas Red-X Phalloidin was used for labeling F-actin and DAPI for labeling the nuclei. This multiple-exposure image was acquired using bandpass optical filter sets appropriate for DAPI, FITC and Texas Red dye.