Brilliant Ultra Violet 496 excitation shown as dotted line and emission shown as solid blue histogram
2355515/30, 450 LP348493(in buffer) 3
 flow cytometry

Invitrogen Brilliant Ultra Violet™ 496 (BUV496) is a tandem dye excited by the 355 nm UV laser and emits at 496 nm. This dye has a low-to-medium relative brightness and can be used for cell surface, intracellular, and nuclear staining.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.

We offer BUV496 dye conjugated to primary antibodies for use in flow cytometry.

Search Brilliant Ultra Violet™ 496 primary antibodies

Experimental data using Brilliant Ultra Violet™ 496

Spectral signature of Brilliant Ultra Violet™ 496 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet™ 496 were used for analysis.

Dot plot of Ki-67 BUV496 vs CD45R (B220) FITC in unstimulated and stimulated cells

Intracellular staining of mouse splenocytes using Brilliant Ultra Violet™ 496. C57BL/6 mouse splenocytes were unstimulated (left) or stimulated for 48 hours with CD3e Monoclonal Antibody, Functional Grade (right). Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody (SolA15), Brilliant Ultra Violet™ 496. Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation.

Dot plot of samples stained with IgG2a control vs CD3e-FITC and CD45R(B220)-BUV496 vs CD3e-FITC

Cell surface staining of C57BL/6 mouse splenocytes using Brilliant Ultra Violet™ 496. C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, FITC and 0.25 µg of Rat IgG2a kappa Isotype Control, Brilliant Ultra Violet™ 496 (left) or 0.25 µg of CD45R Monoclonal Antibody, Brilliant Ultra Violet™ 496 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.

Brilliant Ultra Violet™ dye background

Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.