Brilliant Ultra Violet 563 excitation shown as dotted line and emission shown as solid green-yellow histogram
4355585/15, 535 LP*
560/40, 535 LP**		349563(in buffer) 3
flow cytometry
* Instruments with yellow-green (561nm) laser
** Instruments without yellow-green laser 

Invitrogen Brilliant Ultra Violet™ 563 (BUV563) is a tandem dye excited by the 355 nm UV laser and emits at 563 nm. This dye has a medium-to-high relative brightness and can be used for cell surface, intracellular, and nuclear staining.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye-conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.

We offer BUV563 dye conjugated to primary antibodies for use in flow cytometry.

Search Brilliant Ultra Violet™ 563 primary antibodies

Experimental data using Brilliant Ultra Violet™ 563

Spectral signature of Brilliant Ultra Violet™ 563 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD3 Monoclonal Antibody (SK7), Brilliant Ultra Violet™ 563 were used for analysis. .

Dot plot of Ki-67 BUV563 vs CD45R (B220) FITC in unstimulated and stimulated cells

Intracellular staining of mouse splenocytes using Brilliant Ultra Violet™ 563. C57BL/6 mouse splenocytes were unstimulated (left) or stimulated for 48 hours with CD3e Monoclonal Antibody, Functional Grade (right). Cells were then surface-stained with CD45R (B220) Monoclonal Antibody, FITC and stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set and protocol, with 1.0 µg of Ki-67 Monoclonal Antibody (SolA15), Brilliant Ultra Violet™ 563. Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Blue Dead Cell Stain Kit, for UV excitation.

Dot plot of samples stained with IgG1 control vs CD3-FITC and CD19-BUV563 vs CD3-FITC

Cell surface staining of human peripheral blood cells using Brilliant Ultra Violet™ 563. Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet™ 563 (left) or CD19 Monoclonal Antibody, Brilliant Ultra Violet™ 563 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.

Brilliant Ultra Violet™ dye background

Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.