Brilliant Ultra Violet 805 excitation shown as dotted line and emission shown as solid red histogram
3355780/60349804(in buffer) 4
 flow cytometry

Invitrogen Brilliant Ultra Violet™ 805 (BUV805) is a tandem dye excited by the 355 nm UV laser and emits at 805 nm far red. This dye has a low-to-medium relative brightness and can be used for cell surface and intracellular staining. Nuclear targets may not be expressed at high enough levels to be paired with this dye.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.

We offer BUV805 dye conjugated to primary antibodies for use in flow cytometry.

Search Brilliant Ultra Violet™ 805 primary antibodies

Experimental data using Brilliant Ultra Violet™ 805

Spectral signature of Brilliant Ultra Violet™ 805 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD4 Monoclonal Antibody (RM4-5), Brilliant Ultra Violet™ 805 were used for analysis.

 

Dot plot of IFN-gamma-BUV805 vs CD4-APC in unstimulated and stimulated cells

Intracellular staining of human peripheral blood cells using Brilliant Ultra Violet™ 805. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (right). Cells were then stained intracellularly, using Intracellular Fixation & Permeabilization Buffer Set, CD4 Monoclonal Antibody (SK3 (SK-3)), APC and IFN gamma Monoclonal Antibody, Brilliant Ultra Violet™ 805. Viable cells in the lymphocyte gate were used for analysis, as determined by LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit, for 405 nm excitation.

 

Dot plots of samples stained with IgG1 control vs CD3-FITC and CD4-BUV805 vs CD3-FITC

Cell surface staining of human peripheral blood cells using Brilliant Ultra Violet™ 805. Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, FITC and Mouse IgG1 kappa Isotype Control, Brilliant Ultra Violet™ 805 (left) or CD4 Monoclonal Antibody, Brilliant Ultra Violet™ 805 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.

Brilliant Ultra Violet™ dye background

Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.