Brilliant Violet 650 excitation shown as dotted line and emission shown as solid red histogram
2405660/20, 630 LP407646(in buffer) 3
 flow cytometry

Invitrogen Brilliant Violet™ 650 (BV650) is a tandem dye excited by the 405 nm violet laser and emits at 650 nm. This dye has a low-to-medium relative brightness and can be used for cell surface, intracellular, and nuclear staining. BV650 may have some cross-laser excitation with the red laser.

When using two or more Super Bright, Brilliant Violet™, Brilliant Ultra Violet™, or other polymer dye- conjugated antibodies in a staining panel, we recommend using Super Bright Complete Staining Buffer or Brilliant Stain Buffer to minimize any non-specific polymer interactions.

We offer BV650 dye conjugated to primary antibodies for use in flow cytometry.

Search Brilliant Violet™ 650 primary antibodies

Experimental data using Brilliant Violet™ 650

Spectral signature of Brilliant Violet™ 650 dye. Data acquired on a 5-laser Cytek Aurora and normal human peripheral blood cells stained with CD8a Monoclonal Antibody (RPA-T8), Brilliant Violet™ 650 were used for analysis.

 

Dot plot of TNF-alpha-BV650 vs CD4-FITC in unstimulated and stimulated cells

Intracellular staining of human peripheral blood cells using Brilliant Ultra Violet™ 650. Normal human peripheral blood cells were unstimulated (left) or stimulated for 5 hours with the Cell Stimulation Cocktail (plus protein transport inhibitors) (right). Cells were then stained intracellularly, using Intracellular Fixation & Permeabilization Buffer Set, CD4 Monoclonal Antibody (RPA-T4), FITC and TNF alpha Monoclonal Antibody, Brilliant Violet™ 650. Viable cells in the lymphocyte gate were used for analysis, as determined by Fixable Viability Dye eFluor™ 780.

 

Dot plot of samples stained with IgG2a control vs CD3e-FITC and CD4-BV650 vs CD3e-FITC

Cell surface staining of mouse splenocytes using Brilliant Violet™ 650. C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, FITC and 0.25 µg of Rat IgG2a kappa Isotype Control, Brilliant Violet™ 650 (left) or 0.25 µg of CD4 Monoclonal Antibody (RM4-5), Brilliant Violet™ 650 (right). Viable cells in the lymphocyte gate were used for analysis, as determined by 7-AAD Viability Staining Solution.

 

Brilliant Ultra Violet™ dye background

Brilliant Ultra Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry as well as traditional flow cytometry. The UV laser has unique channels far from heavily occupied detectors, allowing for larger panels.