eFluor 450 excitation shown as dotted line and emission shown as solid blue histogram
2405450/50405445(in buffer) 1flow cytometry

The eFluor 450 dye is a violet-excitable, organic dye that emits at 450 nm and has spectral and performance properties similar to Pacific Blue dye. It exhibits equal or better fluorescence intensity when compared to Horizon V500 dye or Pacific Blue dye in multicolor applications. Stability data shows eFluor 450 exhibits minimal loss of fluorescence, when cells are exposed to a formaldehyde fixative treatment for up to 24 hours.

We offer eFluor 450 dye conjugated to primary antibodies for use in flow cytometry.

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Benefits of eFluor 450 dye
  • Equal or better fluorescence intensity when compared to several other violet-excited organic dyes
  • Stable fluorescence following aldehyde fixation
  • Helps extend options for multicolor panel design to the violet laser
Experimental data using eFluor 450 dye
Flow histogram comparing eFluor 450 dye to Pacific Blue dye

Comparison of fluorescence intensity of eFluor 450 dye with Pacific Blue. Human peripheral blood cells (PBMCs) were stained with CD45RA Monoclonal Antibody (HI100), eFluor 450 (purple line) or CD45RA Monoclonal Antibody (MEM-56), Pacific Blue (blue dotted line).

Flow histogram comparing eFluor 450 dye before and after aldehyde fixation
Stability of eFluor 450 dye. Mouse splenocytes were stained with CD4 Monoclonal Antibody (RM4-5), eFluor 450 and analyzed immediately (red line). The sample was then fixed in 2% formaldehyde overnight at 4°C and analyzed again (green line).