NovaFluor Blue 555 excitation shown as dotted line and emission shown as solid green histogram
2488542/18493555(in buffer) 5
flow cytometry

Invitrogen NovaFluor Blue 555 dye is a yellow-green fluorescent dye with an excitation and emission profile similar to Alexa Fluor 532 dye, but is 2.5 times brighter. This dye should generally be paired with highly expressed antigens to minimize spectral spillover in complex multicolor panels. NovaFluor 555 dye can be used in flow cytometry panels that include both NovaFluor Blue 510 and NovaFluor Blue 530 dyes. 

The macromolecule-based NovaFluor Blue 555 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes. 

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.  

Search NovaFluor Blue 555 primary antibodies

Benefits of NovaFluor Blue 555 dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Can serve as a brighter replacement for Alexa Fluor 532 dye
  • Can be used in a flow cytometry panel with NovaFluor Blue 510 and NovaFluor Blue 530 dyes
  • Produces highly stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor Blue 555 dye
Spectral signature of NovaFluor Blue 555 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Blue 555 were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Side scatter plot with unstained and CD19 NovaFluor Blue 555-stained normal human PBMCs
Staining of human peripheral blood cells with NovaFluor Blue 555 conjugated antibody. Normal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD19 Monoclonal Antibody, NovaFluor Blue 555 (right). Data was acquired in the B3 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.