Novafluor Blue 610-30S excitation shown as dotted line and emission shown as solid orange histogram
2488610/20508615(in buffer) 5flow cytometry

NovaFluor Blue 610 dyes are available with two different levels of brightness: NovaFluor Blue 610-30S and NovaFluor Blue 610-70S, allowing for differential matching of the dye brightness to the antigen density or abundance, without panel redesign. NovaFluor Blue 610-30S and NovaFluor Blue 610-70S dyes have separate spectral signatures but are difficult to use in the same panel. NovaFluor Blue 610-30S is dimmer than NovaFluor 610-70S.

NovaFluor Blue 610-30S dye can be used to replace PE/Dazzle 594, BD Horizon PE-CF594, or PE-Texas Red dye using the 488 nm blue laser line for excitation. As compared with these PE tandem dyes, NovaFluor Blue 610-30S dye shows much less cross-excitation and lower spectral spillover with the 561 nm yellow-green laser line, allowing detection of an additional marker labeled with, for example, NovaFluor Yellow 610 dye. The macromolecule-based NovaFluor Blue 610 dyes produce highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C. Use NovaFluor dyes with CellBlox Plus Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

Search NovaFluor Blue 610-30S primary antibodies

Benefits of NovaFluor Blue 610-30S dye
  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Lower intensity allows it to be paired with highly expressed antigens
  • Can serve as a replacement for PE-tandem dyes using the blue laser, with reduced cross-laser excitation and fluorescent spillover
  • Single PE-tandems can be replaced by NovaFluor Blue 610-30S and NovaFluor Yellow 610 dyes to allow detection of an additional marker in a flow cytometry panel
  • NovaFluor Blue 610-30S and NovaFluor Blue 610-70S can be used together and unmixed using a spectral flow cytometer
  • Produces highly stable fluorescence and is compatible with all buffers tested
Experimental data using NovaFluor Blue 610-30S dye
Graph of relative intensity vs wavelength for NovaFluor Blue 610-30S dye

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Blue 610-30S dye.

Graph of fluorescence intensity vs channel for NovaFluor Blue 610-30S dye

Figure 2. Spectral signature of NovaFluor Blue 610-30S dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Blue 610-30S dye were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.

Side scatter plot with unstained and CD19 NovaFluor Blue 610-30S-stained normal human PBMCs

Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD19 Monoclonal Antibody, NovaFluor Blue 610-30S (right). Data were acquired in the B6 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.