NovaFluor Yellow 660 excitation shown as dotted line and emission shown as solid red histogram
3561670/14550664(in buffer) 5
flow cytometry

Invitrogen NovaFluor Yellow 660 dye has been optimized for flow cytometers with spectral capabilities. Phiton technology provides highly stable fluorescence when stored at 4°C. NovaFluor Yellow 660 dye can be used to replace PE-tandems such as PE-Cy5 or PE-Fire™ 640 dye, with much less cross-excitation and fluorescent spillover. Replacement of PE-tandems with NovaFluor Yellow 660 dye, which is primarily excited by the yellow-green laser, opens a detector, allowing for an additional marker to be detected off of the blue laser line using NovaFluor Blue 660 dye.

Use NovaFluor dyes with CellBlox Plus Blocking Buffer to reduce background and to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

Search NovaFluor Yellow 660 primary antibodies

Benefits of NovaFluor Yellow 660 dye
  • Can be paired with medium to lowly expressed antigens
  • Replacement for PE-Cy5 with reduced cross-laser excitation and fluorescent spillover
  • PE-Cy5 can be replaced by NovaFluor Blue 660 and NovaFluor Yellow 660 dyes for larger conventional flow panels
  • Highly stable and compatible with all tested buffers
Experimental data using NovaFluor Yellow 660 dye

Spectral signature of NovaFluor Yellow 660 dye. Normal human peripheral blood cells stained with CD4 Monoclonal Antibody (SK3 (SK-3)), NovaFluor Yellow 660 were used for analysis. Data were acquired on a 5-laser Cytek Aurora system.
 

Side Scatter plot with unstained and CD3 NovaFluor Yellow 660-stained normal human PBMCs

Staining of human peripheral blood cells with NovaFluor Yellow 660 dye conjugated antibodyNormal human peripheral blood cells (PBMCs) were unstained (left) or stained with CD3 Monoclonal Antibody (UCHT1), NovaFluor Yellow 660 (right). Data were acquired in the YG4 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.