Qdot 605 spectra excitation shown as dotted line and emission shown as solid yellow histogram
5350, 488, 561, 594605/20300603(in buffer) 5microscopy, flow cytometry

Invitrogen Qdot 605 probe is an excellent red-fluorescent label with outstanding brightness and photostability for imaging and flow cytometry applications. With an emission maximum of ~605 nm, Qdot 605 probe can be excited using 350, 488, 561, or 594 nm laser lines or a standard TRITC filter. Optimum imaging results with brighter signals are achieved with a Qdot 605 filter or light cube.

Organic fluorophores and Qdot probes can be readily combined in multiplexed flow cytometry or imaging studies. Longer-wavelength Qdot probes are also well-suited for multiplexed western blots.

We offer Qdot 605 probe conjugated to a variety of antibodies, peptides, and proteins, optimized for cellular labeling and detection. In addition, reactive dye forms and antibody labeling kits are available to allow you to generate your own antibody conjugates or probes.

Qdot Label Conjugates for Cell and Tissue Staining    Qdot 605 primary antibodies

Experimental data using Qdot 605 probe
Flow scatter plot of Qdot 605-stained CD3 vs. Qdot 705-stained CD56

Flow cytometry analysis of whole blood cells using streptavidin conjugates of Qdot 605 and Qdot 705. Whole blood cells were lysed with ammonium chloride, then sequentially stained with mouse anti-human CD3 (Biotin), Qdot 605 Streptavidin Conjugate, mouse anti-human CD56 (Biotin), and Qdot 705 Streptavidin Conjugate, with washes between each step. The cells were resuspended and analyzed by flow cytometry using 405 nm excitation, and detected using 605/20 nm and 710/20 nm emission bandpass filters. The dot plot shows Qdot 705 streptavidin conjugate on the Y-axis and Qdot 605 streptavidin conjugate on the X-axis.