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Gibco Protein Expression Basics
Gibco Protein Expression Basics is an online hub that brings together educational content and tools to enhance your research. Here you can access our protein expression selection guide, handbook, educational videos, and technical resources. Start here if you are new to protein expression or need to refresh your knowledge.
Do you need guidance on a new project, troubleshooting expertise, or more information on a product?
Share your protein expression application needs with us, and one of our protein expression specialists will contact you to discuss the solutions we can provide.
Find your optimal expression system
Our interactive tool allows you to compare protein expression systems side-by-side, find the one that matches your yield and timeline goals, and see the products needed for a successful outcome.
Protein Expression Handbook
This protein expression handbook will help you choose the right expression system and purification technologies for your specific application and needs. Get tips and tricks when starting an experiment, and find answers to everyday problems related to protein expression.
Gibco protein expression webinars
Watch on-demand webinars with leading industry experts and see the latest advances in protein expression.
COVID-19 Serology Assays and Cell Therapy Strategies
Presented by Fatima Amanat (Department of Microbiology, Icahn School of Medicine at Mount Sinai), Dean Lee (Director of the Cellular Therapy and Cancer Immunology Program and Physician at Nationwide Children's Hospital), and Lior Raviv (Vice President Development & Operations, Pluristem Therapeutics Inc.)
Harnessing the Power of Protein Expression Systems in Vaccine Development
Presented by Roland Leathers, PhD, Senior Manager, Strategic Alliances Europe, India & Australasia, Stem Cell Systems & Drug Discovery Solutions, Thermo Fisher Scientific
Streamlining the Biologics Development Process: From Transient to Stable Production in the ExpiCHO System
Presented by Jonathan Zmuda, Ph.D.ExpiSf Expression System: A Chemically defined baculovirus-based expression system for enhanced protein and virus production in Sf9 cells
Presented by Ken Thompson, PhD
Expression, purification, and characterization of difficult to express membrane proteins in the Expi293 and ExpiCHO expression systems
Presented by Anass Jawhari, PhD, Chief Scientific Officer, CALIXAR
Enabling next-generation targeted siRNA therapies with ExpiCHO
Presented by Ian Huggins, PhD, Post-Doctoral Fellow, UC San Diego
Factor V expression in ExpiCHO cells
Presented by Laurent Mosnier, PhD, Associate Professor, The Scripps Research Institute
Expi293: new tools for structural biology and difficult to express proteins
Presented by Jon Zmuda, PhD, Director Cell Biology R&D, Thermo Fisher Scientific
Gibco ExpressionWorld 2016: Development of a mammalian recombinant protein production suite
Gibco ExpressionWorld 2016: Streamlining the drug development process with the ExpiCHO system
Protein Expression Publication Hub
Protein expression systems enable high-yield, consistent protein production for a diverse array of downstream applications. Protein solubility, functionality, purification speed, and yield are crucial factors to consider when choosing an expression system. Thermo Fisher Scientific drives innovation in the protein expression space, accelerating the development of new platforms that offer integrated solutions to accommodate your research needs.
This publication hub offers an in-depth look into how scientists like you advance their research with Expi protein expression systems. Our systems are optimized to harness the power of transient expression with the goal of delivering high-yield, consistent proteins for biotechnology, gene therapy, immunotherapy, immunology, structural, functional, vaccine, and infectious disease research.
Search the publication hub by filtering based on application, host type or expression system, or by entering a search term below.
Find your optimal expression system
Our interactive tool allows you to compare protein expression systems side-by-side, find the one that matches your yield and timeline goals, and see the products needed for a successful outcome.
Protein Expression Handbook
This protein expression handbook will help you choose the right expression system and purification technologies for your specific application and needs. Get tips and tricks when starting an experiment, and find answers to everyday problems related to protein expression.
Gibco protein expression webinars
Watch on-demand webinars with leading industry experts and see the latest advances in protein expression.
COVID-19 Serology Assays and Cell Therapy Strategies
Presented by Fatima Amanat (Department of Microbiology, Icahn School of Medicine at Mount Sinai), Dean Lee (Director of the Cellular Therapy and Cancer Immunology Program and Physician at Nationwide Children's Hospital), and Lior Raviv (Vice President Development & Operations, Pluristem Therapeutics Inc.)
Harnessing the Power of Protein Expression Systems in Vaccine Development
Presented by Roland Leathers, PhD, Senior Manager, Strategic Alliances Europe, India & Australasia, Stem Cell Systems & Drug Discovery Solutions, Thermo Fisher Scientific
Streamlining the Biologics Development Process: From Transient to Stable Production in the ExpiCHO System
Presented by Jonathan Zmuda, Ph.D.ExpiSf Expression System: A Chemically defined baculovirus-based expression system for enhanced protein and virus production in Sf9 cells
Presented by Ken Thompson, PhD
Expression, purification, and characterization of difficult to express membrane proteins in the Expi293 and ExpiCHO expression systems
Presented by Anass Jawhari, PhD, Chief Scientific Officer, CALIXAR
Enabling next-generation targeted siRNA therapies with ExpiCHO
Presented by Ian Huggins, PhD, Post-Doctoral Fellow, UC San Diego
Factor V expression in ExpiCHO cells
Presented by Laurent Mosnier, PhD, Associate Professor, The Scripps Research Institute
Expi293: new tools for structural biology and difficult to express proteins
Presented by Jon Zmuda, PhD, Director Cell Biology R&D, Thermo Fisher Scientific
Gibco ExpressionWorld 2016: Development of a mammalian recombinant protein production suite
Gibco ExpressionWorld 2016: Streamlining the drug development process with the ExpiCHO system
Protein Expression Publication Hub
Protein expression systems enable high-yield, consistent protein production for a diverse array of downstream applications. Protein solubility, functionality, purification speed, and yield are crucial factors to consider when choosing an expression system. Thermo Fisher Scientific drives innovation in the protein expression space, accelerating the development of new platforms that offer integrated solutions to accommodate your research needs.
This publication hub offers an in-depth look into how scientists like you advance their research with Expi protein expression systems. Our systems are optimized to harness the power of transient expression with the goal of delivering high-yield, consistent proteins for biotechnology, gene therapy, immunotherapy, immunology, structural, functional, vaccine, and infectious disease research.
Search the publication hub by filtering based on application, host type or expression system, or by entering a search term below.
Gibco Protein Expression Basics videos
Table of Contents
Part 1 - Protein expression overview
- How proteins are made
- Cloning technologies for protein expression
- Transformation and plasmid isolation
- Selecting an expression system
- Cell lysis and protein purification
Part 2 – Mammalian cell-based protein expression
- Transient, high-yield mammalian expression
- Stable protein expression for large-scale, commercial bioproduction
- Targeted protein expression for stable cell line development
- Membrane protein production
- Viral delivery for mammalian expression
Part 3 – Insect cell-based protein expression
Resources
Education & Support