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Flow Cytometry Protocols |
Preparing for your flow cytometry experiment
General protocols for flow cytometry
- Super Bright Staining Buffer protocol
- Cell Preparation for Flow Cytometry Protocols (Invitrogen eBioscience reagents)
- Red Blood Cell Lysis Protocols Using eBioscience Lysis Buffers (Invitrogen eBioscience reagents)
- Staining Cell Surface Targets for Flow Cytometry (Invitrogen eBioscience reagents)
- Staining Intracellular Antigens for Flow Cytometry (Invitrogen eBioscience reagents)
- OneComp and UltraComp Compensation Beads Protocols for Flow Cytometry (Invitrogen eBioscience reagents)
Cell preparation protocols for single cell suspensions
- Isolation of human peripheral blood mononuclear cells from whole blood
- Monocyte isolation from peripheral blood mononuclear cells
- NK cell isolation from peripheral blood mononuclear cells
- T cell isolation from peripheral blood mononuclear cells
- CD4+ T cell isolation from peripheral blood mononuclear cells
- CD8+ T cell isolation from peripheral blood mononuclear cells
- Regulatory T cell isolation from peripheral blood mononuclear cells
- T cell isolation from secondary lymphoid organs
- Immune cell counting using automated cell counters
- Mouse Bone Marrow Cell Isolation
- Mouse Spleen Cell Isolation Protocol
- Whole Blood Staining Protocol for Flow Cytometry Analysis
Immune cell stimulation protocols
- In vitro differentiation of macrophages from monocytes via M-CSF
- T-cell activation via functional antibodies anti-CD3 and anti-CD28
- Immune cell activation for cytokine production via LPS
- Stimulation of cytokine production in immune cells
- T-cell activation via Concanavalin A
- T-cell activation with Dynabeads Human T-Activator CD3/CD28
Cell viability protocols for flow cytometry
Cell viability and cell vitality protocols for flow cytometry
- LIVE/DEAD Violet Viability/Vitality Kit Protocol
- Annexin V Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)
- Pharmacological Induction of Apoptosis with Camptothecin (Invitrogen eBioscience reagents)
Cell proliferation protocols for flow cytometry
- CellTrace CFSE Cell Proliferation Kit Protocol
- CellTrace Far Red Cell Proliferation Kit Protocol
- CellTrace Violet Cell Proliferation Kit Protocol
- BrdU and EdU Double Staining Protocol
- Click-iT EdU Protocol for Flow Cytometry
- Click-iT Plus EdU Flow Cytometry Assay Kits
- BrdU Staining Protocol for Flow Cytometry (Invitrogen eBioscience reagents)
- Staining Cells with eFluor Proliferation Dyes for Flow Cytometry (Invitrogen eBioscience reagents)