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Bethyl Laboratories
IRF3 Polyclonal Antibody
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IRF3 Antibody (A303-384A) in WB
Detection of mouse IRF3 by western blot and immunoprecipitation. Samples: Whole cell lysate from mouse bone marrow dendritic (D; 50 and 15 µg for WB; 1 mg for IP, 20% of IP loaded), and NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-IRF3 antibody (Product # A303-384A) used for WB at 0.4 µg/mL (A) and 1 µg/mL (B) and used for IP at 6 µg/mg lysate. IRF3 was also immunoprecipitated by rabbit anti-IRF3 antibody A303-383A, which recognizes a disparate epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A... View More
Product Details
A303-384A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 375 and 419 of mouse Interferon Regulatory Factor 3.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
tris citrate/phosphate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
IFR3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IFR3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and plays a critical role in both the early and late phases of the IFNA/B gene induction. IFR3 is found in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA, or toll-like receptor signaling. It is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), a complex which activates the transcription of the type I IFN and ISG genes. Mutations in the gene can result in encephalopathy, acute, infection-induced, herpes-specific 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Interferon regulatory factor 3; IRF-3; IRF3 (phospho S386); IRF3 (pS386); IRF3 (pSer386); IRF3 phospho; IRF3 phospho S386; IRF3 phospho Ser386; phospho IRF3
Gene Aliases: C920001K05Rik; IRF-3; Irf3
UniProt ID: (Mouse) P70671
Entrez Gene ID: (Mouse) 54131
negative regulation of transcription from RNA polymerase II promoter
immune system process
transcription, DNA-templated
regulation of transcription, DNA-templated
response to bacterium
lipopolysaccharide-mediated signaling pathway
response to lipopolysaccharide
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to exogenous dsRNA
innate immune response
type I interferon biosynthetic process
negative regulation of interferon-beta biosynthetic process
negative regulation of defense response to virus by host
positive regulation of cytokine secretion
defense response to virus
positive regulation of type I interferon-mediated signaling pathway
cellular response to lipopolysaccharide
programmed necrotic cell death
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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