Proteintech

MDMX Polyclonal Antibody

FIGURE: 1 / 6

MDMX Antibody (17914-1-AP) in WB

Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 17914-1-AP (MDMX antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Product Details

17914-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 22 publications 22 publications

Immunohistochemistry (IHC)

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

MDMX Fusion Protein Ag12319 (1-332 aa encoded by BC067299)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.23 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: MTSFSTSAQ CSTSDSACRI SPGQINQVRP KLPLLKILHA AGAQGEMFTV KEVMHYLGQY IMVKQLYDQQ EQHMVYCGGD LLGELLGRQS FSVKDPSPLY DMLRKNLVTL ATATTDAAQT LALAQDHSMD IPSQDQLKQS AEESSTSRKR TTEDDIPTLP TSEHKCIHSR EDEDLIENLA QDETSRLDLG FEEWDVAGLP WWFLGNLRSN YTPRSNGSTD LQTNQDVGTA IVSDTTDDLW FLNESVSEQL GVGIKVEAAD TEQTSEEVGK VSDKKVIEVG KNDDLEDSKS LSDDTDVEVT SEDEWQCTEC KKFNSPSKRY CFRCWALRKD WYS (1-332 aa encoded by BC067299)

Target Information

The MDM2 protein is the primary regulator of p53 protein stability. MDMX is an MDM2-related protein that inhibits MDM2-mediated degradation of p53 via distinct associations with MDM2. The gene that encodes MDMX (also designated MDM4) is a target for amplification in malignant gliomas. ARF interacts with MDMX to sequester MDMX within the nucleolus. This sequestration of MDMX by ARF results in an increase in p53 transactivation. In addition, expression of MDMX can reverse MDM2-targeted degradation of p53 while maintaining suppression of p53 transactivation. Like MDM2, MDMX also binds p73 and stabilizes the level of p73. Therefore, in striking contrast to p53, the half-life of p73 is increased by binding to MDM2.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Structure-based discovery of novel α-aminoketone derivatives as dual p53-MDM2/MDMX inhibitors for the treatment of cancer.

European journal of medicinal chemistry

Luo HJ,Si DJ,Sun XJ,Wang MY,Yang YB,Wang B,Wen HM,Li W,Liu J

Wed Apr 05 00:00:00 UTC 2023

SPY1 inhibits neuronal ferroptosis in amyotrophic lateral sclerosis by reducing lipid peroxidation through regulation of GCH1 and TFR1.

Cell death and differentiation

Wang D,Liang W,Huo D,Wang H,Wang Y,Cong C,Zhang C,Yan S,Gao M,Su X,Tan X,Zhang W,Han L,Zhang D,Feng H

Wed Feb 01 00:00:00 UTC 2023

Loss of RanGAP1 drives chromosome instability and rapid tumorigenesis of osteosarcoma.

Developmental cell

Gong Y,Zou S,Deng D,Wang L,Hu H,Qiu Z,Wei T,Yang P,Zhou J,Zhang Y,Zhu W,Xie X,Liao Z,Yang J,Zhang S,Liu A,Jiang Y,Zou Z,Bai X

Mon Feb 06 00:00:00 UTC 2023

Lin28a maintains a subset of adult muscle stem cells in an embryonic-like state.

Cell research

Wang P,Liu X,Yao Z,Chen Y,Luo L,Liang K,Tan JE,Chua MJ,Chua YB,Ma S,Zhang L,Ma W,Liu S,Cao W,Guo L,Guang L,Wang Y,Zhao H,Ai N,Li Y,Li C,Wang RR,Teh BT,Jiang L,Yu K,Shyh-Chang N

Fri Sep 01 00:00:00 UTC 2023

Dual Targeting of MDM4 and FTH1 by MMRi71 for Induced Protein Degradation and p53-Independent Apoptosis in Leukemia Cells.

Molecules (Basel, Switzerland)

Lama R,Galster SL,Xu C,Davison LW,Chemler SR,Wang X

Tue Nov 08 00:00:00 UTC 2022

RNAi-Mediated Screen of Primary AML Cells Nominates MDM4 as a Therapeutic Target in NK-AML with DNMT3A Mutations.

Cells

Sidorova OA,Sayed S,Paszkowski-Rogacz M,Seifert M,Camgöz A,Roeder I,Bornhäuser M,Thiede C,Buchholz F

Wed Mar 02 00:00:00 UTC 2022

The inhibitory effect and mechanism of Yi-qi-hua-yu-jie-du decoction on the drug resistance of gastric cancer stem cells based on ABC transporters.

Chinese medicine

Huang W,Wen F,Gu P,Liu J,Xia Y,Li Y,Zhou J,Song S,Ruan S,Gu S,Chen X,Shu P

Tue Aug 09 00:00:00 UTC 2022

Small-Molecule MMRi62 Induces Ferroptosis and Inhibits Metastasis in Pancreatic Cancer via Degradation of Ferritin Heavy Chain and Mutant p53.

Molecular cancer therapeutics

Li J,Lama R,Galster SL,Inigo JR,Wu J,Chandra D,Chemler SR,Wang X

Fri Apr 01 00:00:00 UTC 2022

Structure-Based Discovery of MDM2/4 Dual Inhibitors that Exert Antitumor Activities against MDM4-Overexpressing Cancer Cells.

Journal of medicinal chemistry

Zhang S,Yan Z,Li Y,Gong Y,Lyu X,Lou J,Zhang D,Meng X,Zhao Y

Thu Apr 28 00:00:00 UTC 2022

miR-1307-5p suppresses proliferation and tumorigenesis of bladder cancer via targeting MDM4 and the Hippo signaling pathway.

Discover oncology

Huang W,Zhang C,Xiong S,Zhou X,Wang G,Guo J

Fri Jul 01 00:00:00 UTC 2022

MDM2 E3 ligase activity is essential for p53 regulation and cell cycle integrity.

PLoS genetics

Chinnam M,Xu C,Lama R,Zhang X,Cedeno CD,Wang Y,Stablewski AB,Goodrich DW,Wang X

Sun May 01 00:00:00 UTC 2022

Design, synthesis and biological evaluation of novel pyrrolidone-based derivatives as potent p53-MDM2 inhibitors.

Bioorganic chemistry

Si D,Luo H,Zhang X,Yang K,Wen H,Li W,Liu J

Fri Oct 01 00:00:00 UTC 2021

Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.

Brain : a journal of neurology

Kim H,Shin JY,Jo A,Kim JH,Park S,Choi JY,Kang HC,Dawson VL,Dawson TM,Shin JH,Lee Y

Fri Dec 31 00:00:00 UTC 2021

Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.

Brain : a journal of neurology

Kim H,Shin JY,Jo A,Kim JH,Park S,Choi JY,Kang HC,Dawson VL,Dawson TM,Shin JH,Lee Y

Fri Dec 31 00:00:00 UTC 2021

Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.

Brain : a journal of neurology

Kim H,Shin JY,Jo A,Kim JH,Park S,Choi JY,Kang HC,Dawson VL,Dawson TM,Shin JH,Lee Y

Fri Dec 31 00:00:00 UTC 2021

Parkin interacting substrate phosphorylation by c-Abl drives dopaminergic neurodegeneration.

Brain : a journal of neurology

Kim H,Shin JY,Jo A,Kim JH,Park S,Choi JY,Kang HC,Dawson VL,Dawson TM,Shin JH,Lee Y

Fri Dec 31 00:00:00 UTC 2021

Exogenous spermine attenuates rat diabetic cardiomyopathy via suppressing ROS-p53 mediated downregulation of calcium-sensitive receptor.

Redox biology

Wang Y,Chen J,Li S,Zhang X,Guo Z,Hu J,Shao X,Song N,Zhao Y,Li H,Yang G,Xu C,Wei C

Fri May 01 00:00:00 UTC 2020

Context-dependent roles of MDMX (MDM4) and MDM2 in breast cancer proliferation and circulating tumor cells.

Breast cancer research : BCR

Gao C,Xiao G,Piersigilli A,Gou J,Ogunwobi O,Bargonetti J

Mon Jan 14 00:00:00 UTC 2019

Context-dependent roles of MDMX (MDM4) and MDM2 in breast cancer proliferation and circulating tumor cells.

Breast cancer research : BCR

Gao C,Xiao G,Piersigilli A,Gou J,Ogunwobi O,Bargonetti J

Mon Jan 14 00:00:00 UTC 2019

Context-dependent roles of MDMX (MDM4) and MDM2 in breast cancer proliferation and circulating tumor cells.

Breast cancer research : BCR

Gao C,Xiao G,Piersigilli A,Gou J,Ogunwobi O,Bargonetti J

Mon Jan 14 00:00:00 UTC 2019

Context-dependent roles of MDMX (MDM4) and MDM2 in breast cancer proliferation and circulating tumor cells.

Breast cancer research : BCR

Gao C,Xiao G,Piersigilli A,Gou J,Ogunwobi O,Bargonetti J

Mon Jan 14 00:00:00 UTC 2019

Combination of dihydromyricetin and ondansetron strengthens antiproliferative efficiency of adriamycin in K562/ADR through downregulation of SORCIN: A new strategy of inhibiting P-glycoprotein.

Journal of cellular physiology

Sun Y,Liu W,Wang C,Meng Q,Liu Z,Huo X,Yang X,Sun P,Sun H,Ma X,Peng J,Liu K

Mon Apr 01 00:00:00 UTC 2019

Combination of dihydromyricetin and ondansetron strengthens antiproliferative efficiency of adriamycin in K562/ADR through downregulation of SORCIN: A new strategy of inhibiting P-glycoprotein.

Journal of cellular physiology

Sun Y,Liu W,Wang C,Meng Q,Liu Z,Huo X,Yang X,Sun P,Sun H,Ma X,Peng J,Liu K

Mon Apr 01 00:00:00 UTC 2019

MiR-1205 functions as a tumor suppressor by disconnecting the synergy between KRAS and MDM4/E2F1 in non-small cell lung cancer.

American journal of cancer research

Yan H,Chen X,Li Y,Fan L,Tai Y,Zhou Y,Chen Y,Qi X,Huang R,Ren J

Mon Jul 15 00:00:00 UTC 2024

Long Non-coding RNA SNHG12 Functions as a Competing Endogenous RNA to Regulate MDM4 Expression by Sponging miR-129-5p in Clear Cell Renal Cell Carcinoma.

Frontiers in oncology

Wu Z,Chen D,Wang K,Cao C,Xu X

Thu Oct 01 00:00:00 UTC 2020

Mdm2 Splice isoforms regulate the p53/Mdm2/Mdm4 regulatory circuit via RING domain-mediated ubiquitination of p53 and Mdm4.

Cell cycle (Georgetown, Tex.)

Fan C,Wang X

Mon Apr 03 00:00:00 UTC 2017

Mdm2 Splice isoforms regulate the p53/Mdm2/Mdm4 regulatory circuit via RING domain-mediated ubiquitination of p53 and Mdm4.

Cell cycle (Georgetown, Tex.)

Fan C,Wang X

Mon Apr 03 00:00:00 UTC 2017

Targeting P-glycoprotein function, p53 and energy metabolism: Combination of metformin and 2-deoxyglucose reverses the multidrug resistance of MCF-7/Dox cells to doxorubicin.

Oncotarget

Xue C,Wang C,Sun Y,Meng Q,Liu Z,Huo X,Sun P,Sun H,Ma X,Ma X,Peng J,Liu K

Tue Jan 31 00:00:00 UTC 2017

Reversal of cisplatin resistance in non-small cell lung cancer stem cells by Taxus chinensis var.

Genetics and molecular research : GMR

Jiang YQ,Xu XP,Guo QM,Xu XC,Liu QY,An SH,Xu JL,Su F,Tai JB

Fri Sep 02 00:00:00 UTC 2016

Reversal of cisplatin resistance in non-small cell lung cancer stem cells by Taxus chinensis var.

Genetics and molecular research : GMR

Jiang YQ,Xu XP,Guo QM,Xu XC,Liu QY,An SH,Xu JL,Su F,Tai JB

Fri Sep 02 00:00:00 UTC 2016

EZH2 inhibition re-sensitizes multidrug resistant B-cell lymphomas to etoposide mediated apoptosis.

Oncoscience

Smonskey M,Lasorsa E,Rosario S,Kirk JS,Hernandez-Ilizaliturri FJ,Ellis L

Mon Mar 14 00:00:00 UTC 2016

Targeting RING domains of Mdm2-MdmX E3 complex activates apoptotic arm of the p53 pathway in leukemia/lymphoma cells.

Cell death & disease

Wu W,Xu C,Ling X,Fan C,Buckley BP,Chernov MV,Ellis L,Li F,Muñoz IG,Wang X

Thu Dec 31 00:00:00 UTC 2015

Targeting RING domains of Mdm2-MdmX E3 complex activates apoptotic arm of the p53 pathway in leukemia/lymphoma cells.

Cell death & disease

Wu W,Xu C,Ling X,Fan C,Buckley BP,Chernov MV,Ellis L,Li F,Muñoz IG,Wang X

Thu Dec 31 00:00:00 UTC 2015

FL118 induces p53-dependent senescence in colorectal cancer cells by promoting degradation of MdmX.

Cancer research

Ling X,Xu C,Fan C,Zhong K,Li F,Wang X

Mon Dec 15 00:00:00 UTC 2014

FL118 induces p53-dependent senescence in colorectal cancer cells by promoting degradation of MdmX.

Cancer research

Ling X,Xu C,Fan C,Zhong K,Li F,Wang X

Mon Dec 15 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: DKFZp781B1423; double minute 4 homolog; Double minute 4 protein; double minute 4, human homolog of; p53-binding protein; human homolog of; Mdm2-like p53-binding protein; Mdm4 p53 binding protein homolog; MDM4 protein variant G; MDM4 protein variant Y; MDM4-related protein 1; MGC132766; p53-binding protein Mdm4; Protein Mdm4; Protein Mdmx; RP11-430C7.1; transformed mouse 3T3 cell double minute 4

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Gene Aliases: 4933417N07Rik; AA414968; AL023055; AU018793; AU021806; C85810; HDMX; MDM4; MDMX; MRP1

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UniProt ID: (Human) O15151, (Rat) Q5XIN1, (Mouse) O35618

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Entrez Gene ID: (Human) 4194, (Rat) 304798, (Mouse) 17248

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Function(s)

protein binding zinc ion binding enzyme binding ubiquitin protein ligase activity molecular_function

Process(es)

negative regulation of transcription from RNA polymerase II promoter protein complex assembly biological_process cell proliferation positive regulation of cell proliferation DNA damage response, signal transduction by p53 class mediator negative regulation of protein catabolic process negative regulation of apoptotic process G0 to G1 transition protein stabilization negative regulation of cell cycle arrest cellular response to hypoxia DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest negative regulation of cell proliferation protein ubiquitination regulation of signal transduction by p53 class mediator

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MDMX Polyclonal Antibody

MDMX Antibody (17914-1-AP) in WB

Product Details

17914-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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