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Invitrogen
NOXA Polyclonal Antibody
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NOXA Antibody (PA1-41010) in IHC (P)
Immunohistochemical analysis of NOXA in formalin fixed paraffin-embedded (FFPE) human tonsil. Samples were incubated in NOXA polyclonal antibody (Product # PA1-41010) using a dilution of 1:50. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10 mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The se... View More
Product Details
PA1-41010
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptides corresponding to residues 51-66 and 75-90 of mouse Noxa.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2166692
Product Specific Information
Suggested positive control: human, mouse or rat thymus tissue lysate or protein, antigen standard for PMAIP1 (transient overexpression lysate).
Target Information
Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified, which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9. Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53. Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adult T cell leukemia-derived PMA-responsive; Immediate-early-response protein APR; Phorbol-12-myristate-13-acetate-induced protein 1; PMA-ind; PMA-induced protein 1; Protein Noxa
Gene Aliases: APR; NOXA; PMAIP1
UniProt ID: (Human) Q13794, (Mouse) Q9JM54
Entrez Gene ID: (Human) 5366, (Mouse) 58801
release of cytochrome c from mitochondria
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
cellular response to DNA damage stimulus
intrinsic apoptotic signaling pathway in response to DNA damage
response to UV
response to X-ray
negative regulation of mitochondrial membrane potential
intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
regulation of apoptotic process
T cell homeostasis
positive regulation of apoptotic process
positive regulation of DNA damage response, signal transduction by p53 class mediator
positive regulation of neuron apoptotic process
negative regulation of fibroblast proliferation
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
intrinsic apoptotic signaling pathway by p53 class mediator
positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
protein insertion into mitochondrial membrane involved in apoptotic signaling pathway
proteasomal protein catabolic process
positive regulation of glucose metabolic process
positive regulation of protein oligomerization
cellular response to glucose starvation
positive regulation of cysteine-type endopeptidase activity involved in apoptotic process
response to dsRNA
regulation of mitochondrial membrane permeability
defense response to virus
cellular response to hypoxia
reactive oxygen species metabolic process
positive regulation of release of cytochrome c from mitochondria
intrinsic apoptotic signaling pathway
positive regulation of extrinsic apoptotic signaling pathway via death domain receptors
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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