Invitrogen

NOXA Monoclonal Antibody (114C307.1)

NOXA Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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NOXA Antibody (MA1-41000) in IHC (P)

Immunohistochemical analysis of NOXA in Bouin-fixed, paraffin-embedded Chronic Lymphocytic Leukemia (CLL) xenograft. Samples were incubated in NOXA monoclonal antibody (Product # MA1-41000) using a dilution of 1:2000 followed by a peroxidase-conjugate and DAB chromogen at a dilution of . Staining for 2 hr at RT. Image using the azide-free form of this antibody.

Product Image Gallery

NOXA Antibody — Altered expression of proteins upon cell treatment demonstrates antibody specificity. Western blot using NOXA Monoclonal Antibody (114C307.1) (Product # MA1-41000) shows increase in expression of Noxa (6kDa isoform) upon IFN gamma treatment in HAEC cells [doi:doi:10.1038/mi.2017.117] {TM}

Product Details

MA1-41000

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Immunocytochemistry (ICC/IF)

1:20-1:1,000

Flow Cytometry (Flow)

1 µg/ million cells

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

114C307.1

Immunogen

Human Noxa.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.0 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1077295

Product Specific Information

Suggested positive control: antigen standard for PMAIP1 (transient overexpression lysate), RL-7, Jurkat.

Target Information

Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified, which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9. Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53. Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

NOXA expression is downregulated in human breast cancer undergoing incomplete pathological response and senescence after neoadjuvant chemotherapy.

Scientific reports

Al Shboul S,El-Sadoni M,Alhesa A,Abu Shahin N,Abuquteish D,Abu Al Karsaneh O,Alsharaiah E,Ismail MA,Tyutyunyk-Massey L,Alotaibi MR,Neely V,Harada H,Saleh T

MA1-41000 was used in Western Blot to investigate the change in expression of PAMIP1, the gene encoding for the pro-apoptotic protein, NOXA, following NAC in two breast cancer gene datasets, and the change in NOXA protein expression in response to NAC in 55 matched patient samples (pre- and post-NAC).

Sat Sep 23 00:00:00 UTC 2023

PUMA and NOXA Expression in Tumor-Associated Benign Prostatic Epithelial Cells Are Predictive of Prostate Cancer Biochemical Recurrence.

Cancers

Clairefond S,Péant B,Ouellet V,Barrès V,Tian Z,Trudel D,Karakiewicz PI,Mes-Masson AM,Saad F

MA1-41000 was used in Western Blotting to show that the combination of PUMA and NOXA protein expression in benign epithelial cells was predictive of recurrence following radical prostatectomy and was independent of PSA at diagnosis, Gleason score and pathologic stage.

Thu Oct 29 00:00:00 UTC 2020

Venetoclax Is Effective in Small-Cell Lung Cancers with High BCL-2 Expression.

Clinical cancer research : an official journal of the American Association for Cancer Research

Lochmann TL,Floros KV,Naseri M,Powell KM,Cook W,March RJ,Stein GT,Greninger P,Maves YK,Saunders LR,Dylla SJ,Costa C,Boikos SA,Leverson JD,Souers AJ,Krystal GW,Harada H,Benes CH,Faber AC

MA1-41000 was used in Western Blotting to identify venetoclax-sensitive small-cell lung cancers and analyse venetoclax's action in these cancers and its anticancer effect.

Mon Jan 15 00:00:00 UTC 2018

Chaperone-mediated autophagy promotes lung cancer cell survival through selective stabilization of the pro-survival protein, MCL1.

Biochemical and biophysical research communications

Suzuki J,Nakajima W,Suzuki H,Asano Y,Tanaka N

MA141000 was used in western blot to explore the role of chaperone-mediated autophagy in non-small-cell lung cancer

Sun Jan 22 00:00:00 UTC 2017

Combination with vorinostat overcomes ABT-263 (navitoclax) resistance of small cell lung cancer.

Cancer biology & therapy

Nakajima W,Sharma K,Hicks MA,Le N,Brown R,Krystal GW,Harada H

MA141000 was used in western blot to study the effects of HDAC inhibitors and BCL-2 inhibitors on small cell lung cancer lines

Mon Oct 31 00:00:00 UTC 2016

Cisplatin-induced apoptosis in non-small-cell lung cancer cells is dependent on Bax- and Bak-induction pathway and synergistically activated by BH3-mimetic ABT-263 in p53 wild-type and mutant cells.

Biochemical and biophysical research communications

Matsumoto M,Nakajima W,Seike M,Gemma A,Tanaka N

MA141000 was used in western blot to elucidate the mechanism of cisplatin-induced non-small-cell lung cancer cell apoptosis

Fri Apr 29 00:00:00 UTC 2016

DNA damaging agent-induced apoptosis is regulated by MCL-1 phosphorylation and degradation mediated by the Noxa/MCL-1/CDK2 complex.

Oncotarget

Nakajima W,Sharma K,Lee JY,Maxim NT,Hicks MA,Vu TT,Luu A,Yeudall WA,Tanaka N,Harada H

MA141000 was used in western blot to report that Noxa-mediated MCL-1 phosphorylation and subsequent MCL-1 degradation is critical for DNA damaging agent-induced apoptosis

Tue Jun 14 00:00:00 UTC 2016

Noxa determines localization and stability of MCL-1 and consequently ABT-737 sensitivity in small cell lung cancer.

Cell death & disease

Nakajima W,Hicks MA,Tanaka N,Krystal GW,Harada H

MA1-41000 was used in western blot to evaluate the effect of Noxa on the localization and stability of MCL-1 in small cell lung cancer

Thu Feb 13 00:00:00 UTC 2014

BRAF inhibitors suppress apoptosis through off-target inhibition of JNK signaling.

eLife

Vin H,Ojeda SS,Ching G,Leung ML,Chitsazzadeh V,Dwyer DW,Adelmann CH,Restrepo M,Richards KN,Stewart LR,Du L,Ferguson SB,Chakravarti D,Ehrenreiter K,Baccarini M,Ruggieri R,Curry JL,Kim KB,Ciurea AM,Duvic M,Prieto VG,Ullrich SE,Dalby KN,Flores ER,Tsai KY

mA1-41000 was used in western blot to study the role of off-target JNK inhibition in the mechanism by which BRAF inhibitors block apoptosis

Tue Nov 05 00:00:00 UTC 2013

Proteasomal degradation of Mcl-1 by maritoclax induces apoptosis and enhances the efficacy of ABT-737 in melanoma cells.

PloS one

Pandey MK,Gowda K,Doi K,Sharma AK,Wang HG,Amin S

MA1-41000 was used in western blot to study the effects of the Mcl-1 inhibitor Maritoclax and ABT-737 on melanoma cell survival

Fri Aug 08 00:00:00 UTC 2014

Bioinformatics

Protein Aliases: adult T cell leukemia-derived PMA-responsive; Immediate-early-response protein APR; Phorbol-12-myristate-13-acetate-induced protein 1; PMA-ind; PMA-induced protein 1; Protein Noxa

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Gene Aliases: APR; NOXA; PMAIP1

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UniProt ID: (Human) Q13794, (Mouse) Q9JM54, (Rat) Q5U777

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Entrez Gene ID: (Human) 5366, (Mouse) 58801, (Rat) 492821

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Function(s)

protein binding molecular_function

Process(es)

Performance Guarantee

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NOXA Monoclonal Antibody (114C307.1)

NOXA Antibody (MA1-41000) in IHC (P)

Product Details

MA1-41000

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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