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Invitrogen
Perforin Monoclonal Antibody (eBioBOR21), eBioscience™
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Perforin Antibody (14-9993-82) in WB
Western Blot was performed using Anti-Perforin Monoclonal Antibody (eBioBOR21), eBioscience™ (Product # 14-9993-82), and a 61 kDa band corresponding to Perforin-1 was observed in the NK-92 cell line but not in the A-431 cell line as reported. An uncharacterized band (*) at ~110 kDa was also observed in the A-431 cell line. Whole-cell extracts (30 µg lysate) of NK-92 (Lane 1), NK-92 treated with PTI (1X for 4h) (Lane 2), A-431 (Lane 3) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins... View More
Product Details
14-9993-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
eBioBOR21
Conjugate
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_468675
Product Specific Information
Description: The eBioBOR21 antibody clone reacts with human perforin (pore-forming protein, pfp). Perforin is one of the cytolytic mediators present in the cytoplasmic granules of cytotoxic T lymphocytes (CTL) and natural killer cells (NK). Perforin is involved in the killing function by CTLs and NKs and has an important role in the immune response against tumors and virus infections.
Applications Tested: The eBioBOR21 antibody has been tested for use in immunoblotting (WB) of human PBMCs at 1-5 µg/mL. It is recommended that this antibody be titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Perforin is one of the major cytolytic proteins of cytolytic granules. Perforin is a cytolytic mediator and is stored in and released by cytoplasmic granules. Moreover, perforin is involved in immune defense against tumors and virus infections as mediated by cytotoxic lymphocytes. Perforin is a 555 amino acid protein with a 21 amino acid signal peptide, and has a molecular weight of 70 to 75 kD. Perforin is a pore forming protein with a mechanism of transmembrane channel formation similar to C9, and homology between perforin and C9 have been demonstrated. Studies show that perforin is expressed only in killer cell lines and not in helper T lymphocytes or other tumor cells tested. Perforin is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in the perforin gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. Alternative splicing results in multiple transcript variants of perforin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cytolysin; lymphocyte pore forming protein; Lymphocyte pore-forming protein; OMAK; OTTHUMP00000019759; P1; perforin 1 (pore forming protein); Perforin-1; Perforin1; PFP; PGFL; PIGF; PIGF-2; PLGF; PRF1 (pore forming protein 1); RP11-710A11.3
Gene Aliases: FLH2; HPLH2; P1; PFN1; PFP; PRF1
UniProt ID: (Human) P14222
Entrez Gene ID: (Human) 5551
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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