Invitrogen

Phospho-ATM (Ser1981) Monoclonal Antibody (10H11)

Advanced Verification
1 Published Figure
5 References
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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA1-2020

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:1,000
View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

1:250
View 2 publications 2 publications

Immunoprecipitation (IP)

Assay-dependent
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10H11

Immunogen

Synthetic peptide corresponding to residues S(1974) L A F E E S(p) Q S T T I S S(1988) of human ATM Kinase protein.
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1086244

Product Specific Information

MA1-2020 detects the phospho-ATM kinase in human and mouse samples.

MA1-2020 has been successfully used in immunofluorescence, immunoprecipitation and Western blot procedures. By Western blot this antibody detects a ~370 kDa protein representing the phospho-ATM kinase in crude lysates from gamma irradiated HeLa cells. In immunofluorescence procedures, MA1-2020 recognizes the phospho-ATM kinase in irradiated human and mouse fibroblasts.

The MA1-2020 immunogen is a phosphorylated synthetic peptide corresponding to the residues S(1974) L A F E E S(p) Q S T T I S S(1988) of human ATM Kinase protein.

Target Information

Ataxia-telangiectasia Mutated (ATM) is a protein that belongs to the PI3/PI4 kinase family. Ataxia-telangiectasia is a rare autosomal recessive disorder characterized by progressive neurologic degeneration, immunologic deficiency, and an increased risk of lymphoid cancer. The ATM gene codes for a protein belonging to the phosphoinositide 3-kinase (PI3K) superfamily. ATM phosphorylates proteins instead of lipid and has many downstream targets that act as cell-cycle regulators including: P53, Mdm2, BRCA1, and SMC1. The ATM protein is responsible for repairing double-stranded DNA breaks that occur because of ionizing radiation and other mutagens. The ATM’s C-terminal region has extensive homology to the catalytic domains of phosphatidylinositol 3-kinases (PI3 kinases). Studies have shown that ATM becomes autophosphorylated and upregulated by exposure to ionizing radiation. AT cells are hypersensitive to ionizing radiation, impaired in mediating the inhibition of DNA synthesis and display delays in p53 induction. Further, DNA damage caused by ionizing irradiation activates ATM-kinase, leading to a cascade of kinase reactions that regulate cell cycle, apoptosis, and DNA damage repair. Studies have linked ATM to apoptosis along with Nbs1 and Chk2 in the E2F1 pathway.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: A-T mutated; A-T mutated homolog; AT mutated; AT mutated protein; Ataxia t; ataxia telangiectasia gene mutated in human beings; Ataxia telangiectasia mutated; Ataxia telangiectasia mutated homolog; DKFZp781A0353; EC 2.7.1.37; MGC74674; Serine-protein kinase ATM; TEL1, telomere maintenance 1, homolog

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Gene Aliases: AI256621; AT1; ATA; ATC; ATD; ATDC; ATE; ATM; C030026E19Rik; TEL1; TELO1

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UniProt ID: (Human) Q13315, (Mouse) Q62388

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Entrez Gene ID: (Human) 472, (Mouse) 11920

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Function(s)
DNA binding protein serine/threonine kinase activity DNA-dependent protein kinase activity protein binding ATP binding 1-phosphatidylinositol-3-kinase activity protein complex binding protein dimerization activity protein N-terminus binding nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups phosphotransferase activity, alcohol group as acceptor histone serine kinase activity
Process(es)
DNA damage checkpoint telomere maintenance double-strand break repair via homologous recombination response to hypoxia somitogenesis pre-B cell allelic exclusion DNA repair protein phosphorylation cellular response to DNA damage stimulus DNA damage induced protein phosphorylation cell cycle cell cycle arrest mitotic spindle assembly checkpoint female gamete generation brain development heart development determination of adult lifespan intrinsic apoptotic signaling pathway in response to DNA damage response to ionizing radiation phosphorylation histone phosphorylation peptidyl-serine phosphorylation negative regulation of B cell proliferation V(D)J recombination histone-serine phosphorylation phosphatidylinositol-3-phosphate biosynthetic process lipoprotein catabolic process positive regulation of apoptotic process positive regulation of DNA damage response, signal transduction by p53 class mediator positive regulation of neuron apoptotic process protein autophosphorylation phosphatidylinositol phosphorylation oocyte development neuron apoptotic process regulation of cell cycle histone mRNA catabolic process cellular response to gamma radiation signal transduction involved in mitotic G2 DNA damage checkpoint replicative senescence DNA double-strand break processing DNA synthesis involved in DNA repair strand displacement DNA replication double-strand break repair via nonhomologous end joining DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest telomere maintenance via telomerase reciprocal meiotic recombination signal transduction regulation of autophagy positive regulation of telomere maintenance via telomerase positive regulation of histone phosphorylation peptidyl-serine autophosphorylation regulation of apoptotic process meiotic telomere clustering regulation of telomerase activity cellular response to X-ray cellular response to nitrosative stress establishment of RNA localization to telomere establishment of macromolecular complex localization to telomere regulation of cellular response to heat regulation of signal transduction by p53 class mediator negative regulation of TORC1 signaling negative regulation of telomere capping positive regulation of telomere maintenance via telomere lengthening positive regulation of telomerase catalytic core complex assembly
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