Invitrogen

Phospho-SMAD1/SMAD5 (Ser463, Ser465) Monoclonal Antibody (E.239.4)

2 Published Figures
2 References
View all (5) SMAD1/SMAD5 antibodies

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Datasheet
Protocols
Questions & Answers
Datasheet
Protocols
Questions & Answers

Product Details

MA5-15124

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:800
-

Flow Cytometry (Flow)

1:400-1:1,600
-
Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

E.239.4

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding pSer463/465 of human Smad5
View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

148 µg/mL

Purification

Affinity chromatography

Storage buffer

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

Contains

<0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11000599

Product Specific Information

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other Smad-related proteins.

Target Information

The Smad family of proteins are functioning in the transmission of extracellular signals in the TGF-beta signaling pathway. Binding of a TGF-beta superfamily ligands to extracellular receptors triggers phosphorylation of Smad2 at a Serine-Serine-Methionine-Serine (SSMS) motif at its C-terminus. Phosphorylated Smad2 is then able to form a complex with Smad4. These complexes accumulate in the cell nucleus, where they are directly participating in the regulation of gene expression. In mammals, eight Smad proteins have been identified to date. The Smad family of proteins can be divided into three functional groups: the receptor-activated Smads (R-Smads), common mediator Smads (Co-Smads), and the inhibitory Smads (I-Smads). The R-Smads are directly phosphorylated by the activated type I receptors on their C-terminal Ser-Ser-X-Ser (SSXS) motif and include Smad1, Smad2, Smad3, Smad5, and Smad8. Smad2 and Smad3 are phosphorylated in response to TGF-beta and activin, whereas Smad1, Smad5, and Smad8 are phosphorylated in response to BMP (Bone Morphogenetic Protein). This C-terminal phosphorylation allows R-Smad binding to Co-Smad, Smad4, and translocation to the nucleus where they regulate TGF-beta target genes. Smad6 and Smad7 belong to the I-Smad which bind to the type I receptor or Smad4 and block their interaction with R-Smads.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: BSP-1; Dwarfin-A; Dwarfin-C; Dwf-A; Dwf-C; hSMAD1; hSmad5; JV4-1; JV5-1; MAD (mothers against decapentaplegic, Drosophila) homolog 1; MAD homolog 1; MAD homolog 5; MAD homolog1 (mothers against decapentaplegic, Drosophila); MAD, mothers against decapentaplegic homolog 1; MAD, mothers against decapentaplegic homolog 5; Mad-related protein 1; mMad1; Mothers against decapentaplegic homolog 1; Mothers against decapentaplegic homolog 5; mothers against decapentaplegic, drosophila, homolog of, 5; mothers against DPP homolog 1; mothers against DPP homolog 5; Mothers-against-DPP-related 1; mSmad5; OTTHUMP00000223331; SMA- and MAD-related protein 5; SMAD; SMAD 1; SMAD 5; SMAD family member 1; SMAD family member 5; SMAD, mothers against DPP homolog 1; SMAD, mothers against DPP homolog 5; TGF-beta signaling protein 1; transforming growth factor-beta signaling protein 1; Transforming growth factor-beta-signaling protein 1

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Gene Aliases: 1110051M15Rik; AI451355; AI528653; BSP-1; BSP1; Dwf-C; DWFC; JV4-1; JV41; JV5-1; Mad1; MADH1; MADH5; MADR1; Mlp1; Msmad5; MusMLP; SMAD1; SMAD5

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UniProt ID: (Human) Q15797, (Human) Q99717, (Mouse) P70340, (Rat) P97588, (Mouse) P97454, (Rat) Q9R1V3

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Entrez Gene ID: (Human) 4086, (Human) 4090, (Mouse) 17125, (Rat) 25671, (Mouse) 17129, (Rat) 59328

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Function(s)
RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core promoter sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding receptor signaling protein activity protein binding protein kinase binding transforming growth factor beta receptor, pathway-specific cytoplasmic mediator activity identical protein binding protein homodimerization activity metal ion binding protein heterodimerization activity co-SMAD binding I-SMAD binding ubiquitin protein ligase binding DNA binding
Process(es)
ureteric bud development Mullerian duct regression osteoblast fate commitment transcription, DNA-templated protein phosphorylation signal transduction transforming growth factor beta receptor signaling pathway germ cell development embryonic pattern specification erythrocyte differentiation BMP signaling pathway positive regulation of osteoblast differentiation positive regulation of transcription, DNA-templated cartilage development cardiac muscle contraction bone development SMAD protein signal transduction cellular response to organic cyclic compound positive regulation of transcription from RNA polymerase II promoter involved in cellular response to chemical stimulus angiogenesis regulation of transcription, DNA-templated multicellular organism development cell differentiation positive regulation of transcription from RNA polymerase II promoter cellular response to BMP stimulus MAPK cascade mesodermal cell fate commitment transcription from RNA polymerase II promoter inflammatory response SMAD protein complex assembly gamete generation negative regulation of cell proliferation positive regulation of gene expression midbrain development hindbrain development primary miRNA processing homeostatic process cardiac muscle cell proliferation positive regulation of cartilage development regulation of transcription from RNA polymerase II promoter response to drug positive regulation of cell differentiation kidney development response to organonitrogen compound negative regulation of muscle cell apoptotic process wound healing positive regulation of dendrite morphogenesis ossification regulation of ossification
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