Invitrogen

RUNX3 Monoclonal Antibody (2B3)

RUNX3 Antibody in Immunocytochemistry (ICC/IF)

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RUNX3 Antibody (MA5-17169) in ICC/IF

Immunofluorescence analysis of RUNX3 was performed using 70% confluent log phase SW480 and Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with RUNX3 Monoclonal Antibody (2B3) (Product # MA5-17169) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Produ... View More

RUNX3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

RUNX3 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HCT 116 cells were transfected with RUNX3 siRNA and reduction of signal was observed in Western Blot using RUNX3 Monoclonal Antibody (Product # MA5-17169). {KD}

Product Details

MA5-17169

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000

Immunocytochemistry (ICC/IF)

1:100-1:1,000

Flow Cytometry (Flow)

1:200-1:400

ELISA (ELISA)

1:10,000

ChIP assay (ChIP)

2.5 µg/10^6 cells

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

2B3

Immunogen

Purified recombinant fragment of human RUNX3 (amino acids:186-252) expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2538640

Product Specific Information

MA5-17169 targets RUNX3 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human and Mouse samples.

The MA5-17169 immunogen is purified recombinant fragment of human RUNX3 (amino acids:186-252) expressed in E. Coli.

MA5-17169 detects RUNX3 which has a predicted molecular weight of approximately 44.4kDa.

Target Information

This gene encodes a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Expression levels of RUNX3 and FGFR2 in peripheral blood of severe acute pancreatitis and their clinical significance.

Annals of surgical treatment and research

Liang Y,Ding H

MA5-17169 was used in ChIP Assay, ELISA, Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to conclude that RUNX3 and FGFR2 were downregulated in peripheral blood of severe acute pancreatitis (SAP) patients and served as candidate biomarkers for SAP diagnosis.

Wed Feb 01 00:00:00 UTC 2023

Bioinformatics

Protein Aliases: Acute myeloid leukemia 2 protein; acute myeloid leukemia gene 2; CBF-alpha 3; CBF-alpha-3; core binding factor alpha 3; Core-binding factor subunit alpha-3; core-binding factor, runt domain, alpha subunit 3; Oncogene AML-2; PEA2 alpha C; PEA2-alpha C; PEBP2 alpha C; PEBP2-alpha C; Polyomavirus enhancer-binding protein 2 alpha C subunit; runt domain, alpha subunit 3; Runt-related transcription factor 3; SL3-3 enhancer factor 1 alpha C subunit; SL3/AKV core-binding factor alpha C subunit; transcription factor AML2; transcription factor AML2/CBFA3

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Gene Aliases: AML2; CBFA3; PEBP2A3; PEBP2aC; RUNX3

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UniProt ID: (Human) Q13761

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Entrez Gene ID: (Human) 864, (Mouse) 12399

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding transcription factor activity, sequence-specific DNA binding protein binding ATP binding DNA binding

Process(es)

negative regulation of transcription from RNA polymerase II promoter ossification chondrocyte differentiation transcription, DNA-templated regulation of transcription, DNA-templated protein phosphorylation axon guidance hemopoiesis neuron differentiation hair follicle morphogenesis neuron projection development interferon-gamma production mRNA transcription from RNA polymerase II promoter regulation of cell differentiation negative regulation of cell cycle cell maturation negative regulation of epithelial cell proliferation positive regulation of extrinsic apoptotic signaling pathway regulation of transcription from RNA polymerase II promoter transcription from RNA polymerase II promoter positive regulation of transcription, DNA-templated peripheral nervous system neuron development response to transforming growth factor beta

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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RUNX3 Monoclonal Antibody (2B3)

RUNX3 Antibody (MA5-17169) in ICC/IF

Product Details

MA5-17169

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

ChIP assay (ChIP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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