Invitrogen

SHP-1 Monoclonal Antibody (2E9E4)

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Datasheet
Protocols
Questions & Answers

Product Details

MA5-38614

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunocytochemistry (ICC/IF)

1:200-1:1,000
-

Flow Cytometry (Flow)

1:200-1:400
-

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

2E9E4

Immunogen

Purified recombinant fragment of human PTPN6 (aa: 243-541) expressed in E. Coli.
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Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein G

Storage buffer

PBS

Contains

0.05% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2898526

Product Specific Information

This antibody has been tested in indirect ELISA.

Target Information

SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: EC 3.1.3.48; hematopoietic cell phosphatase; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6; PTP-1C; SH-PTP1; Tyrosine-protein phosphatase non-receptor type 6

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Gene Aliases: HCP; HCPH; HPTP1C; PTP-1C; PTP1C; PTPN6; SH-PTP1; SHP-1; SHP-1L; SHP1

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UniProt ID: (Human) P29350

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Entrez Gene ID: (Human) 5777

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Function(s)
phosphotyrosine binding protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding SH3 domain binding protein kinase binding SH2 domain binding
Process(es)
hematopoietic progenitor cell differentiation negative regulation of humoral immune response mediated by circulating immunoglobulin protein dephosphorylation apoptotic process G-protein coupled receptor signaling pathway cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation cell differentiation platelet activation platelet formation T cell costimulation abortive mitotic cell cycle positive regulation of cell adhesion mediated by integrin peptidyl-tyrosine dephosphorylation intracellular signal transduction megakaryocyte development negative regulation of T cell proliferation natural killer cell mediated cytotoxicity negative regulation of MAP kinase activity regulation of B cell differentiation negative regulation of peptidyl-tyrosine phosphorylation B cell receptor signaling pathway negative regulation of B cell receptor signaling pathway negative regulation of T cell receptor signaling pathway leukocyte migration regulation of release of sequestered calcium ion into cytosol regulation of type I interferon-mediated signaling pathway regulation of ERK1 and ERK2 cascade platelet aggregation regulation of G1/S transition of mitotic cell cycle
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