Proteintech

SHP1 Polyclonal Antibody

SHP1 Antibody in Immunocytochemistry (ICC/IF)

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SHP1 Antibody (24546-1-AP) in ICC/IF

Immunofluorescent analysis of MCF-7 cells using 24546-1-AP (SHP1 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IGG.

SHP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

SHP1 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

24546-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

View 12 publications 12 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:10-1:100

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

SHP1 Fusion Protein Ag21415 (506-598 aa encoded by BC002523)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.33 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

Immunogen sequence: QYKFIYVAI AQFIETTKKK LEVLQSQKGQ ESEYGNITYP PAMKNAHAKA SRTSSKHKED VYENLHTKNK REEKVKKQRS ADKEKSKGSL KRK (506-598 aa encoded by BC002523)

Target Information

SHP-1 (PTPN6) is a non-receptor protein tyrosine phosphatase that is expressed primarily in hematopoietic cells. The enzyme is composed of two SH2 domains, a tyrosine phosphatase catalytic domain, and a carboxy-terminal regulatory domain. SHP-1 removes phosphates from target proteins to downregulate several tyrosine kinase-regulated pathways. In hematopoietic cells, the amino-terminal SH2 domain of SHP-1 binds to tyrosine phosphorylated erythropoietin receptors (EpoR) to negatively regulate hematopoietic growth. Overexpression of SHP-1 in epithelial cells results in dephosphorylation of the Ros receptor tyrosine kinase and subsequent downregulation of Ros-dependent cell proliferation and transformation. Following ligand binding in myeloid cells, SHP-1 associates with the IL-3R beta chain and downregulates IL-3-induced tyrosine phosphorylation and cell proliferation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance.

Cell reports

Gao X,Wu Y,Chick JM,Abbott A,Jiang B,Wang DJ,Comte-Walters S,Johnson RH,Oberholtzer N,Nishimura MI,Gygi SP,Mehta A,Guttridge DC,Ball L,Mehrotra S,Sicinski P,Yu XZ,Wang H

Tue Apr 25 00:00:00 UTC 2023

Targeting protein tyrosine phosphatases for CDK6-induced immunotherapy resistance.

Cell reports

Gao X,Wu Y,Chick JM,Abbott A,Jiang B,Wang DJ,Comte-Walters S,Johnson RH,Oberholtzer N,Nishimura MI,Gygi SP,Mehta A,Guttridge DC,Ball L,Mehrotra S,Sicinski P,Yu XZ,Wang H

Tue Apr 25 00:00:00 UTC 2023

Src-homology domain 2 containing protein tyrosine phosphatase-1 (SHP-1) directly binds to proto-oncogene tyrosine-protein kinase Src (c-Src) and promotes the transcriptional activation of connexin 43 (Cx43).

Bioengineered

Liu Y,Dai M,Yang P,Cao L,Lu L

Sun May 01 00:00:00 UTC 2022

Host MKRN1-Mediated Mycobacterial PPE Protein Ubiquitination Suppresses Innate Immune Response.

Frontiers in immunology

Dou Y,Xie Y,Zhang L,Liu S,Xu D,Wei Y,Li Y,Zhang XL

Tue May 24 00:00:00 UTC 2022

Molecular features underlying differential SHP1/SHP2 binding of immune checkpoint receptors.

eLife

Xu X,Masubuchi T,Cai Q,Zhao Y,Hui E

Thu Nov 04 00:00:00 UTC 2021

Molecular features underlying differential SHP1/SHP2 binding of immune checkpoint receptors.

eLife

Xu X,Masubuchi T,Cai Q,Zhao Y,Hui E

Thu Nov 04 00:00:00 UTC 2021

CLEC12B suppresses lung cancer progression by inducing SHP-1 expression and inactivating the PI3K/AKT signaling pathway.

Experimental cell research

Chi D,Wang D,Zhang M,Ma H,Chen F,Sun Y

Wed Dec 15 00:00:00 UTC 2021

CLEC12B suppresses lung cancer progression by inducing SHP-1 expression and inactivating the PI3K/AKT signaling pathway.

Experimental cell research

Chi D,Wang D,Zhang M,Ma H,Chen F,Sun Y

Wed Dec 15 00:00:00 UTC 2021

RNF6 promotes myeloma cell proliferation and survival by inducing glucocorticoid receptor polyubiquitination.

Acta pharmacologica Sinica

Ren Y,Xu X,Mao CY,Han KK,Xu YJ,Cao BY,Zhang ZB,Sethi G,Tang XW,Mao XL

Sun Mar 01 00:00:00 UTC 2020

Anticancer activity of dietary xanthone α-mangostin against hepatocellular carcinoma by inhibition of STAT3 signaling via stabilization of SHP1.

Cell death & disease

Zhang H,Tan YP,Zhao L,Wang L,Fu NJ,Zheng SP,Shen XF

Fri Jan 24 00:00:00 UTC 2020

Anticancer activity of dietary xanthone α-mangostin against hepatocellular carcinoma by inhibition of STAT3 signaling via stabilization of SHP1.

Cell death & disease

Zhang H,Tan YP,Zhao L,Wang L,Fu NJ,Zheng SP,Shen XF

Fri Jan 24 00:00:00 UTC 2020

Anticancer activity of dietary xanthone α-mangostin against hepatocellular carcinoma by inhibition of STAT3 signaling via stabilization of SHP1.

Cell death & disease

Zhang H,Tan YP,Zhao L,Wang L,Fu NJ,Zheng SP,Shen XF

Fri Jan 24 00:00:00 UTC 2020

A thiazole-derived oridonin analogue exhibits antitumor activity by directly and allosterically inhibiting STAT3.

The Journal of biological chemistry

Shen X,Zhao L,Chen P,Gong Y,Liu D,Zhang X,Dai L,Sun Q,Lou J,Jin Z,Zhang B,Niu D,Chen C,Qi X,Jia D

Fri Nov 15 00:00:00 UTC 2019

Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.

Oxidative medicine and cellular longevity

Maryam A,Mehmood T,Yan Q,Li Y,Khan M,Ma T

Tue Sep 18 00:00:00 UTC 2018

Proscillaridin A Promotes Oxidative Stress and ER Stress, Inhibits STAT3 Activation, and Induces Apoptosis in A549 Lung Adenocarcinoma Cells.

Oxidative medicine and cellular longevity

Maryam A,Mehmood T,Yan Q,Li Y,Khan M,Ma T

Tue Sep 18 00:00:00 UTC 2018

Chemical and genetic inhibition of STAT3 sensitizes hepatocellular carcinoma cells to sorafenib induced cell death.

International journal of biological sciences

Xie L,Zeng Y,Dai Z,He W,Ke H,Lin Q,Chen Y,Bu J,Lin D,Zheng M

Mon Jul 15 00:00:00 UTC 2019

Chemical and genetic inhibition of STAT3 sensitizes hepatocellular carcinoma cells to sorafenib induced cell death.

International journal of biological sciences

Xie L,Zeng Y,Dai Z,He W,Ke H,Lin Q,Chen Y,Bu J,Lin D,Zheng M

Mon Jul 15 00:00:00 UTC 2019

Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.

Oncotarget

Igbe I,Shen XF,Jiao W,Qiang Z,Deng T,Li S,Liu WL,Liu HW,Zhang GL,Wang F

Tue Dec 26 00:00:00 UTC 2017

Dietary quercetin potentiates the antiproliferative effect of interferon-α in hepatocellular carcinoma cells through activation of JAK/STAT pathway signaling by inhibition of SHP2 phosphatase.

Oncotarget

Igbe I,Shen XF,Jiao W,Qiang Z,Deng T,Li S,Liu WL,Liu HW,Zhang GL,Wang F

Tue Dec 26 00:00:00 UTC 2017

Chemical composition of total flavonoids from Polygonum amplexicaule and their pro-apoptotic effect on hepatocellular carcinoma cells: Potential roles of suppressing STAT3 signaling.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Xiang M,Su H,Hong Z,Yang T,Shu G

Mon Jun 01 00:00:00 UTC 2015

Chemical composition of total flavonoids from Polygonum amplexicaule and their pro-apoptotic effect on hepatocellular carcinoma cells: Potential roles of suppressing STAT3 signaling.

Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association

Xiang M,Su H,Hong Z,Yang T,Shu G

Mon Jun 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: EC 3.1.3.48; hematopoietic cell phosphatase; Hematopoietic cell protein-tyrosine phosphatase; Protein-tyrosine phosphatase 1C; Protein-tyrosine phosphatase SHP-1; PTN6; PTP-1C; SH-PTP1; Tyrosine-protein phosphatase non-receptor type 6

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Gene Aliases: HCP; HCPH; HPTP1C; PTP-1C; PTP1C; PTPN6; SH-PTP1; SHP-1; SHP-1L; SHP1

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UniProt ID: (Human) P29350

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Entrez Gene ID: (Human) 5777

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Function(s)

phosphotyrosine binding protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding SH3 domain binding protein kinase binding SH2 domain binding

Process(es)

hematopoietic progenitor cell differentiation negative regulation of humoral immune response mediated by circulating immunoglobulin protein dephosphorylation apoptotic process G-protein coupled receptor signaling pathway cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation positive regulation of phosphatidylinositol 3-kinase signaling peptidyl-tyrosine phosphorylation cell differentiation platelet activation platelet formation T cell costimulation abortive mitotic cell cycle positive regulation of cell adhesion mediated by integrin peptidyl-tyrosine dephosphorylation intracellular signal transduction megakaryocyte development negative regulation of T cell proliferation natural killer cell mediated cytotoxicity negative regulation of MAP kinase activity regulation of B cell differentiation negative regulation of peptidyl-tyrosine phosphorylation B cell receptor signaling pathway negative regulation of B cell receptor signaling pathway negative regulation of T cell receptor signaling pathway leukocyte migration regulation of release of sequestered calcium ion into cytosol regulation of type I interferon-mediated signaling pathway regulation of ERK1 and ERK2 cascade platelet aggregation regulation of G1/S transition of mitotic cell cycle

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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SHP1 Polyclonal Antibody

SHP1 Antibody (24546-1-AP) in ICC/IF

Product Details

24546-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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