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Invitrogen
Securin Polyclonal Antibody
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FIGURE: 1 / 5
Securin Antibody (PA5-29399) in ICC/IF
Securin Polyclonal Antibody detects PTTG1 protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PTTG1 protein stained by Securin Polyclonal Antibody (Product # PA5-29399) diluted at 1:500. Blue: Hoechst 33342 staining.
Product Details
PA5-29399
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Full length human Securin Recombinant protein.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7, with 1% BSA, 20% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2546875
Product Specific Information
Recommended positive controls: IMR32.
Predicted reactivity: Rhesus Monkey (98%), Chimpanzee (99%), Bovine (85%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Esp1-associated protein; ESP1-associated protein 1; MGC126883; MGC138276; Pituitary tumor-transforming gene 1 protein; Securin; Tumor-transforming protein 1
Gene Aliases: EAP1; HPTTG; PTTG; PTTG1; TUTR1
UniProt ID: (Human) O95997
Entrez Gene ID: (Human) 9232
DNA repair
regulation of transcription, DNA-templated
transcription from RNA polymerase II promoter
mitotic nuclear division
spermatogenesis
negative regulation of endopeptidase activity
anaphase-promoting complex-dependent catabolic process
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
homologous chromosome segregation
chromosome organization
cell division
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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