Invitrogen

Securin Recombinant Rabbit Monoclonal Antibody (19H16L48)

Securin Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 13

Securin Antibody (700791) in ICC/IF

Immunofluorescent analysis of Securin in HeLa cells using a Securin recombinant rabbit monoclonal antibody (Product # 700791) at a dilution of 2 µg/mL followed by detection using an Alexa Fluor 488-conjugated goat anti-rabbit secondary antibody at a dilution of 1:1000. Cells were fixed using 4% paraformaldehyde. The cytoplasmic localization of Securin is shown in green, while nuclei were stained using SlowFade GOLD with DAPI (Product # S36938), shown in blue.

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Product Details

700791

Applications

Tested Dilution

Publications

Western Blot (WB)

1-3 µg/mL

View 6 publications 6 publications

Immunocytochemistry (ICC/IF)

2 µg/mL

View 1 publication 1 publication

Miscellaneous PubMed (MISC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Expression System

Expi293

Class

Recombinant Monoclonal

Type

Antibody

Clone

19H16L48

Immunogen

A recombinant protein corresponding to amino acids 1-202.

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Protein A

Storage buffer

PBS

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

AB_2532345

Product Specific Information

This antibody is predicted to react with bovine based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

Target Information

The encoded protein is a homolog of yeast securin proteins, which prevent separins from promoting sister chromatid separation. It is an anaphase-promoting complex (APC) substrate that associates with a separin until activation of the APC. The gene product has transforming activity in vitro and tumorigenic activity in vivo, and the gene is highly expressed in various tumors. The gene product contains 2 PXXP motifs, which are required for its transforming and tumorigenic activities, as well as for its stimulation of basic fibroblast growth factor expression. It also contains a destruction box (D box) that is required for its degradation by the APC. The acidic C-terminal region of the encoded protein can act as a transactivation domain. The gene product is mainly a cytosolic protein, although it partially localizes in the nucleus.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Centrosome function is critical during terminal erythroid differentiation.

The EMBO journal

Tátrai P,Gergely F

700791 was used in Western Blotting to show that disruption of centrosome and spindle function initiates macrocytic anaemias due to nutritional deficiency or exposure to chemotherapy.

Mon Jul 18 00:00:00 UTC 2022

Age-dependent integrity of the meiotic spindle assembly checkpoint in females requires Aurora kinase B.

Aging cell

Blengini CS,Nguyen AL,Aboelenain M,Schindler K

700791 was used in Western Blotting to demonstrate a novel function for AURKB in preserving the female reproductive lifespan possibly by protecting oocytes from oxidative stress.

Mon Nov 01 00:00:00 UTC 2021

Age-dependent integrity of the meiotic spindle assembly checkpoint in females requires Aurora kinase B.

Aging cell

Blengini CS,Nguyen AL,Aboelenain M,Schindler K

700791 was used in Western Blotting to demonstrate a novel function for AURKB in preserving the female reproductive lifespan possibly by protecting oocytes from oxidative stress.

Mon Nov 01 00:00:00 UTC 2021

Accumulation of Securin on Spindle During Female Meiosis I.

Frontiers in cell and developmental biology

Pauerova T,Radonova L,Horakova A,Knott JG,Anger M

Published figure using Securin recombinant monoclonal antibody (Product # 700791) in Immunocytochemistry

Tue Aug 17 00:00:00 UTC 2021

A unique binding mode of Nek2A to the APC/C allows its ubiquitination during prometaphase.

EMBO reports

Alfieri C,Tischer T,Barford D

700791 was used in Western Blot to show that Nek2A is a conformational-specific binder of the APC/C-MCC complex (APC/CMCC ) and that, in contrast to cyclin A, Nek2A can be ubiquitinated efficiently by the APC/C in conjunction with both the E2 enzymes UbcH10 and UbcH5.

Thu Jun 04 00:00:00 UTC 2020

Cyclin A2 degradation during the spindle assembly checkpoint requires multiple binding modes to the APC/C.

Nature communications

Zhang S,Tischer T,Barford D

700791 was used in Western Blotting to show that a single substrate can engage an E3 ligase through multiple binding modes, affecting its degradation timing and efficiency.

Tue Aug 27 00:00:00 UTC 2019

Elevated PTTG and PBF predicts poor patient outcome and modulates DNA damage response genes in thyroid cancer.

Oncogene

Read ML,Fong JC,Modasia B,Fletcher A,Imruetaicharoenchoke W,Thompson RJ,Nieto H,Reynolds JJ,Bacon A,Mallick U,Hackshaw A,Watkinson JC,Boelaert K,Turnell AS,Smith VE,McCabe CJ

Published figure using Securin monoclonal antibody (Product # 700791) in Western Blot

Thu Sep 14 00:00:00 UTC 2017

Molecular basis of APC/C regulation by the spindle assembly checkpoint.

Nature

Alfieri C,Chang L,Zhang Z,Yang J,Maslen S,Skehel M,Barford D

700791 was used in Western Blotting to study the human anaphase-promoting complex (APC/C)-mitotic checkpoint complex (MCC) structure formed in delayed chromosome segregation using cryo-electron microscopy.

Thu Aug 25 00:00:00 UTC 2016

Potential new chemotherapy strategy for human ovarian carcinoma with a novel KSP inhibitor.

Biochemical and biophysical research communications

Takenaga M,Yamamoto Y,Takeuchi T,Ohta Y,Tokura Y,Hamaguchi A,Asai D,Nakashima H,Oishi S,Fujii N

700791 was used in western blot to describe the anti-cancer effects of KPYB10602.

Fri Jul 31 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: Esp1-associated protein; ESP1-associated protein 1; MGC126883; MGC138276; pituitary tumor-transforming 1; Pituitary tumor-transforming gene 1 protein; Securin; Tumor-transforming protein 1

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Gene Aliases: AW555095; C87862; EAP1; HPTTG; PTTG; PTTG1; Pttg3; TUTR1

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UniProt ID: (Human) O95997, (Mouse) Q9CQJ7

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Entrez Gene ID: (Human) 9232, (Mouse) 30939

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Function(s)

transcription factor activity, sequence-specific DNA binding cysteine-type endopeptidase inhibitor activity protein binding SH3 domain binding peptidase inhibitor activity heat shock protein binding ribosome binding

Process(es)

DNA repair regulation of transcription, DNA-templated transcription from RNA polymerase II promoter mitotic nuclear division spermatogenesis negative regulation of endopeptidase activity anaphase-promoting complex-dependent catabolic process protein ubiquitination involved in ubiquitin-dependent protein catabolic process homologous chromosome segregation chromosome organization cell division regulation of cell growth cellular response to DNA damage stimulus cell cycle chromosome segregation negative regulation of cell proliferation cellular process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Securin Recombinant Rabbit Monoclonal Antibody (19H16L48)

Securin Antibody (700791) in ICC/IF

Product Details

700791

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Miscellaneous PubMed (MISC)

Species Reactivity

Published species

Host/Isotype

Expression System

Class

Type

Clone

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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