MagMAX Kit for HIV-1 Dried Blood Spots

Extract samples from plasma or dried blood spots for HIV-1 drug resistance detection

The Applied Biosystems MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots isolates and purifies nucleic acid from dried blood spots (DBS) or plasma. Dried blood spot is an alternative specimen type that can be shipped at ambient temperature; this cost-effective solution is robust and easily transported to extend the reach of global health and surveillance in remote populations. The Applied Biosystems MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots is compatible with KingFisher automated purification systems and downstream assays such as the HIV-1 Genotyping Kit with Integrase.

The kit utilizes trusted MagMAX magnetic-bead technology, enabling reproducible recovery of high-quality HIV-1 viral RNA compatible with downstream applications such as RT-PCR and Sanger sequencing.

Cost-effective – boost testing volumes in remote regions
Flexible – can be used with plasma or DBS
Convenient – kit contains all reagents from extraction through isolation stored at ambient temperature
High recovery and purity – due to improved sample mixing and washing with magnetic beads
Automated with the KingFisher purification system

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Product details for the Applied Biosystems MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots

Reactions	100 preps
Test duration	~30 min DBS lysis + ~30 min viral RNA extraction
Hands-on time for 24 samples	~30 min
Sample types	EDTA treated plasma, dried blood spots (DBS)
Viral concentration	HIV-1 positive DBS: >2,000 copies/mL HIV-1 positive plasma: >1,000 copies/mL
Kit storage temperature	15 °C to 25 °C
Required equipment	Magnetic stand (manual protocol) KingFisher Sample Purification System (automated protocol)
Regulatory status	Research Use Only (RUO)
Kit components	60 mL Dried Blood Spot Lysis Solution 55 mL Binding Solution 100 mL Wash Solution 10 mL Elution Solution 1 mL Proteinase K 2 mL DNA/RNA Binding Beads

Why use the Applied Biosystems MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots?

Efficiency
- Magnetic bead separation binds nucleic acid more efficiently than glass-fiber filters, resulting in higher and more consistent yields.
- Because filters and vacuum manifolds are not used, there is no risk of filter clogging due to cellular particulates in samples. This clogging issue is of particular concern with protein-rich, large-volume plasma samples that are commonly used for viral testing.
Easy transport
- DBS can be shipped at ambient temperatures. Reduce shipping costs and hassle by shipping samples through regular mail as nonhazardous materials.
- Preserve sample integrity even in transport delays.
Robust
- DBS samples may be more convenient and cost effective in some situations.
Small blood sample
- DBS does not require special equipment for processing (whereas plasma requires centrifuge).
- Compared with venipuncture, DBS samples simplify logistics for sampling and requires smaller volumes of blood (50-100 µL per spot), making samples easier to obtain.