Cover story

Neurons In Focus—Two-photon excitation imaging in live tissue

Nowhere is preserving native cellular context more important than in studies of the nervous system. This issue of BioProbes reviews data and observations from the literature about the dye choices compatible with two-photon excitation (TPE), describes what to consider in making those choices, and lists protocols to optimize their use.

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Also in this issue

New Technologies

  • RNA as you've never seen it before—detect nascent RNA with Click-iT RNAImaging and HCS aAssays
  • Isolate T cells directly from whole blood—Save time with Dynabeads FlowComp isolation technology
  • Chemiluminescent immunodetection and reporter gene assays—High-quality, high-purity substrates for ultrasensitive detection
  • Live-cell imaging of cell cycle and cell division—Introducing the Premo FUCCI Cell Cycle Sensor
  • Advances in neural stem cell culture—Superior NSC expansion with StemPro NSC serum-free medium
  • Optimal growth of neurons and neural stem cells—Gibco Neurobasal media and supplements
  • Ready-to-use, high-quality neural cells—Gibco rat neural primary and stem cells
     

Practical Applications

  • Tools for the study of Alzheimer's disease—Amyloid beta antibodies, peptides, ELISAs, and Luminex® kits
  • A growing list of tools for the violet laser—A guide to violet-excited reagents for flow cytometry
  • A fluorogenic, target-specific reagent for small animal in vivo imaging—Alexa Fluor 680 dye–labeedl probe dramatically improves tumor detection, staging, and monitoring
  • Interrogating potential drug targets for Parkinson's disease—Biochemical and cell-based assays for dopamine D2 and LRRK2
  • Mitochondrial fission and fusion in neurodegeneration
  • Visualizing tumor metastasis
  • Innovative solutions for ion channel research
  • Resources for cytokine research—Antibodies, recombinant proteins, ELISAs and Luminex kits

Departments

  • Journal Highlight—A flow cytometry–based method for isolating neural stem cells
  • On the Web—Find your cellular technology at the Cell Resource Center
  • Just Released—Highlighting our newest cellular analysis products and technologies
  • Endnote—Recently published: A look at how your fellow researchers are using our products
     

Poster

  • Neural Stem Cell Poster—Differentiate your neural stem cell research

For Research Use Only. Not for use in diagnostic procedures.