一旦您在从实验样本中提取和纯化核酸的过程中遇到困难，您最不想做的一件事就是在含有核酸酶的缓冲液中重悬它们或者在下游实验的组份中加入核酸酶。这就是为什么我们提供经过常规质量控制过程生产且经过严格测试的Ambion RNA存储缓冲液和其他缓冲液及试剂。这些试剂已经被证明检测不到非特异性内源核苷酸，外源核苷酸和RNase活性，并满足其他与产品相关的质量要求。
RNase-free TE buffer
Tris-EDTA (TE) is a commonly used pH buffer for labs storing RNA and DNA samples. TE buffer consists of 10 mM Tris (adjusted to the specified pH with HCl) and 1 mM EDTA. These TE buffers are filtered through a 0.2 μm filter into a sterile autoclavable bottle, are nuclease-free, and ready for use without additional preparation.
Features of RNase-free TE buffer include:
- Available in pH 7.0 or 8.0
- Certified RNase-free
- Regular quality control lot testing for contaminating non-specific endonucleases, exonucleases, and RNases
- Manufactured in facilities that are designed to prevent nuclease contamination
RNase-free PBS for cell culture applications
Phosphate-buffered saline (PBS) is a phosphate buffer saline solution used in a cell culture applications. RNase-free PBS helps keep contaminants out of samples and research. PBS that is considered molecular biology grade should not contain contaminants such as proteases, RNases, and DNases.
Features of RNase-free PBS include:
- 10X dilution— ready to use upon dilution to the desired concentration
- Certified RNase-free
- Regular quality control lot testing for contaminating non-specific endonucleases, exonucleases, and RNases
RNA储存和重悬缓冲液
在众多核酸分离实验的最后一步和众多下游反应的第一步都是重悬RNA沉淀。在经过费力的RNA分离之后，将您获得的RNA重悬和储存于正确的无核酸酶的溶液中至关重要。我们提供用于此目的的Ambion RNA储存溶液。这些溶液都是由超纯试剂制备而来，使用我们最先进的生产设备并在严格监控的环境下生产。
Glycogen and GlycoBlue coprecipitant for nucleic acid recovery
Glycogen is a carbohydrate molecule widely used in labs to co-precipitate with RNA or DNA for nucleic acid recovery. Similarly, GlycoBlue coprecipitant has a blue dye molecule linked to glycogen for visibility of the nucleic acid pellet.
Both Glycogen and GlycoBlue coprecipitant have the following features:
- Ideal with RT-PCR
- Increases pellet mass and visibility
- Quantitative recovery of low concentrations (ng/mL) of nucleic acid
- Prevents pellet loss in nuclease protection assays
- RNase- and DNase-free
Proteinase K to inactivate protein activity
Proteinase K is commonly used in molecular biology labs to treat for contaminating nucleases (RNase, DNase). Proteinase K is a serine protease that digests a broad range of proteins.
Recombinant Proteinase K solutions can be used for:
- Preparation of RNA and high molecular weight DNA for gel electrophoresis
- Terminate enzymatic reactions, such as phosphatase or nuclease treatments
- Treatment of samples with high protein concentrations to facilitate downstream phenol extraction
RNase-free EDTA for RNA sample storage
Ethylenediaminetetraacetic acid (EDTA) is a chemical that is universally used in labs for RNA sample storage. Features of RNase-free EDTA include:
- Prepared in nuclease-free (RNase-free, DNase-free), ultrapure water
RNase-free salmon sperm DNA for hybridization experiments
Sheared salmon sperm DNA is a common blocking reagent used in molecular biology labs. It is useful in blocking during northern prehybridization and hybridization. Features of RNase-free salmon sperm DNA include:
- Treated with Proteinase K
- RNase- and DNase-free
RNase-free Tris for electrophoresis assays
Tris solution is used in molecular biology labs in electrophoresis assays. Tris is the main ingredient for TAE (Tris acetate EDTA) and TBE (Tris borate EDTA) buffers regularly used in DNA gel electrophoresis. RNase-free Tris has the following features:
- Available in pH 8.0
- RNase-free
- Regular lot testing for contaminating non-specific endonucleases, exonucleases, and RNases
THE RNA Storage Solution for RNA pellet resuspension
THE RNA Storage Solution is an RNA stabilizing solution used to resuspend and store RNA for future downstream assays, such as:
- RT-PCR
- In vitro transcription
- Northern analysis
- Nuclease protection assays
缓冲液和化学试剂
我们提供多种选择的Ambion缓冲液和试剂，可用于包括RNA和DNA的反应研究中。在您的实验中使用这些经过非特异性内源核酸酶，外源核酸酶和RNase活性经过严格质量控制的产品，将使您更有信心。
仅供科研使用，不可用于诊断目的。