(a) Silencer Select: 赛默飞向您担保，如果您购买了两条靶向同一靶标的Silencer Select预设计siRNA，这两条siRNA均能够沉默70%或更多的靶标mRNA。此担保的前提条件是，siRNA必须在≥5 nM浓度下转染，且必须在转染后48小时测定mRNA水平。推荐采用实时定量RT-PCR技术进行检测。客户还必须证明采用阳性对照siRNA能够充分敲低（>70%的敲低），从而证明转染效率是可行的。我们建议使用Silencer Select非靶向性阴性对照来标准化mRNA的敲低结果。如果未能观测到我们担保的敲低水平，而适当的阳性对照成功敲低，我们将会免费为您合成一条新的Silencer Select siRNA序列。产品更换申请必须在Silencer Select预设计siRNA发货后一百八十(180)天内提出。此担保不包括任何退换产品或定制用于沉默 lncRNAs的siRNA.
(b) Stealth: 赛默飞向您担保，如果您购买了三条靶向同一靶标的Stealth预设计siRNA，这三条独立的非重叠siRNA之中至少有两条能够沉默70%或更多的靶标mRNA。此担保的前提条件是，siRNA必须在≥20 nM浓度下转染，且必须在转染后48小时测定mRNA水平。推荐采用实时定量RT-PCR技术进行检测。客户还必须证明采用阳性对照siRNA能够充分敲低（>70%的敲低），从而证明转染效率是可行的。我们建议您使用适当的阴性对照（如三种Stealth™ RNAi阴性对照之中的一种）来标准化信使敲低水平。如果未能观测到我们担保的敲低水平，而适当的阳性对照成功敲低，我们将会免费为您合成一条新的Stealth siRNA序列。产品更换申请必须在Stealth预设计siRNA发货后一百八十(180)天内提出。此担保不延伸至任何退换产品。
(c) Silencer: 赛默飞向您担保，如果您购买了三条靶向同一靶标的Silencer预设计siRNA，其中至少有两条能够将培养细胞内的靶标mRNA的表达水平降低70%或更高。此担保的前提条件是，在100 nM或更高的siRNA终浓度下进行转染，转染后48小时后测定。客户还必须证明采用阳性对照siRNA能够充分敲低（>70%的敲低），从而证明转染效率是可行的。我们建议使用Silencer非靶向性阴性对照来标准化mRNA的敲低结果。如果三条之中至少有两条未能引起>70%的靶标mRNA敲低效果，赛默飞将会免费一次性更换至多三条靶向Silencer预设计siRNA。产品更换申请必须在Silencer预设计siRNA发货后一百八十(180)天内提出。此担保不延伸至任何退换产品。
*注意：所有担保条款只适用于预设计序列的siRNA产品，定制序列siRNA产品均不享受担保。
siRNA 相关视频
近距离接触发明者系列： Susan Magdaleno探讨Silencer Select siRNA技术
Gavin Robertson介绍 Silencer Select siRNA 技术 如何推进黑色素瘤研究
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仅供科研使用，不可用于诊断目的。