(a) Silencer Select: 赛默飞向您担保,如果您购买了两条靶向同一靶标的Silencer Select预设计siRNA,这两条siRNA均能够沉默70%或更多的靶标mRNA。此担保的前提条件是,siRNA必须在≥5 nM浓度下转染,且必须在转染后48小时测定mRNA水平。推荐采用实时定量RT-PCR技术进行检测。客户还必须证明采用阳性对照siRNA能够充分敲低(>70%的敲低),从而证明转染效率是可行的。我们建议使用Silencer Select非靶向性阴性对照来标准化mRNA的敲低结果。如果未能观测到我们担保的敲低水平,而适当的阳性对照成功敲低,我们将会免费为您合成一条新的Silencer Select siRNA序列。产品更换申请必须在Silencer Select预设计siRNA发货后一百八十(180)天内提出。此担保不包括任何退换产品或定制用于沉默 lncRNAs的siRNA.
(b) Stealth: 赛默飞向您担保,如果您购买了三条靶向同一靶标的Stealth预设计siRNA,这三条独立的非重叠siRNA之中至少有两条能够沉默70%或更多的靶标mRNA。此担保的前提条件是,siRNA必须在≥20 nM浓度下转染,且必须在转染后48小时测定mRNA水平。推荐采用实时定量RT-PCR技术进行检测。客户还必须证明采用阳性对照siRNA能够充分敲低(>70%的敲低),从而证明转染效率是可行的。我们建议您使用适当的阴性对照(如三种Stealth™ RNAi阴性对照之中的一种)来标准化信使敲低水平。如果未能观测到我们担保的敲低水平,而适当的阳性对照成功敲低,我们将会免费为您合成一条新的Stealth siRNA序列。产品更换申请必须在Stealth预设计siRNA发货后一百八十(180)天内提出。此担保不延伸至任何退换产品。
(c) Silencer: 赛默飞向您担保,如果您购买了三条靶向同一靶标的Silencer预设计siRNA,其中至少有两条能够将培养细胞内的靶标mRNA的表达水平降低70%或更高。此担保的前提条件是,在100 nM或更高的siRNA终浓度下进行转染,转染后48小时后测定。客户还必须证明采用阳性对照siRNA能够充分敲低(>70%的敲低),从而证明转染效率是可行的。我们建议使用Silencer非靶向性阴性对照来标准化mRNA的敲低结果。如果三条之中至少有两条未能引起>70%的靶标mRNA敲低效果,赛默飞将会免费一次性更换至多三条靶向Silencer预设计siRNA。产品更换申请必须在Silencer预设计siRNA发货后一百八十(180)天内提出。此担保不延伸至任何退换产品。
*注意:所有担保条款只适用于预设计序列的siRNA产品,定制序列siRNA产品均不享受担保。
siRNA 相关视频
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- Vinod Udayar et al (2013) A Paired RNAi and RabGAP Overexpression Screen Identifies Rab11 as a Regulator of β-Amyloid ProductionCell Reports 5:1552–1563.
- Virginie Buggia-Prévot et al (2013) A Function for EHD Family Proteins in Unidirectional Retrograde Dendritic Transport of BACE1 and Alzheimer's Disease Aβ ProductioCell Reports 5:1536–1551.
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- Casanova CM1, Sehr P, Putzker K, Hentze MW, Neumann B, Duncan KE, Thoma C. (2012) Automated high-throughput RNAi screening in human cells combined with reporter mRNA transfection to identify novel regulators of translationPLoS One 7(9).
- Chrissie Y. Lee, Ronald L. Johnson, Jennifer Wichterman-Kouznetsova, Rajarshi Guha, Marc Ferrer, Pinar Tuzmen, Scott E. Martin, Wenge Zhu, Melvin L. DePamphilis (2012) High-throughput screening for genes that prevent excess DNA replication in human cells and for molecules that inhibit themMethods 57:234–248.
- Bo Rafn, Christian Friberg Nielsen, Sofie Hagel Andersen, Piotr Szyniarowski, Elisabeth Corcelle-Termeau, Erkka Valo, Nicole Fehrenbacher, Charlotta Johanne Olsen, Mads Daugaard, Christina Egebjerg, Trine Bøttzauw, Pekka Kohonen, Jesper Nylandsted, Sampsa Hautaniemi, Jose´ Moreira, Marja Jaattela, and Tuula Kallunki (2012) ErbB2-Driven Breast Cancer Cell Invasion Depends on a Complex Signaling Network Activating Myeloid Zinc Finger-1-Dependent Cathepsin B ExpressionMolecular Cell 45:764–776.
- Alexander V Sirotkin, Dmitriy Ovcharenko, and Milos Mlyncek (2010) Identification of protein kinases that control ovarian hormone release by selective siRNAsJournal of Molecular Endocrinology 44:45–53.
- Thomas Horn, Thomas Sandmann, and Michael Boutros (2010) Design and evaluation of genome-wide libraries for RNA interference screens Genome Biology 11:R61.
仅供科研使用,不可用于诊断目的。