Invitrogen

Human IEX-1 (aa 1-41) Control Fragment Recombinant Protein

Product Details

RP-102947

Applications

Tested Dilution

Publications

Control (Ctrl)

Assay-dependent

Blocking Assay (BLOCK)

Assay-dependent

Product Specifications

Species

Human

Expression System

E. coli

Amino acid sequence

MCHSRSCHPTMTILQAPTPAPSTIPGPRRGSGPEIFTFDPL

Tag

His-ABP-tag

Class

Recombinant

Type

Protein

Purity

>80% by SDS-PAGE and Coomassie blue staining

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

≥5.0 mg/mL

Purification

purified

Storage buffer

PBS/1M urea, pH 7.4

Contains

no preservative

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Product Specific Information

Highest antigen sequence indentity to the following orthologs: Mouse (76%), Rat (76%).

This recombinant protein control fragment may be used for blocking experiments with the corresponding antibody, PA5-60536. In IHC/ICC and WB experiments, we recommend a 100x molar excess of the protein fragment control based on the concentration and the molecular weight. Pre-incubate the antibody-protein control fragment mixture for 30 min at room temperature.

Target Information

IEX-1 is a stress inducible gene that is induced by ionizing radiation, ultraviolet radiation, and a variety of growth factors, i.e., FAS and TNF-a. IEX-1 is widely expressed in epithelial and endocrine tissues, as well as in vascular endothelium. It plays an important role in the regulation of cellular growth, cell death and oncogenesis. IEX-1 is precisely regulated by multiple transcription factors such as p53, NF-kappa-B/rel, Sp1 and c-Myc, to ensure rapid and transient expression of IEX-1 in cells under a variety of stress conditions. IEX-1 is expressed as both a longer form (IEX1L) and a splice variant, designated IEX1S. It is localized to the nucleus and perinuclear region. Overexpression of IEX-1 facilitates apoptosis and cell cycle progression, whereas disruption of IEX-1 expression is associated with decreases in both apoptosis and cell cycle progression.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: anti-death protein; DADB-118P11.5; Differentiation-dependent gene 2 protein; expressed in pancreatic carcinoma; gly96, mouse, homolog of; Immediate early protein GLY96; Immediate early response 3 protein; immediately early gene X-1; PACAP-responsive gene 1 protein; Protein DIF-2; Protein PRG1; Radiation-inducible immediate-early gene IEX-1