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Gibco Human Plasma-like Medium (HPLM) is a new formulation designed to resemble the natural cellular environment found in the body, mimicking the metabolic profile of human plasma.
With the super low cell attachment surface, Thermo Scientific Nunclon Sphera flasks, plates and dishes consistently promote 3D spheroids and organoids culture, aiding you in cancer spheroid formation. See the products
To help guide your 3D research, our R&D teams have developed suggested protocols for the generation of spheroids from the most commonly used cancer cell lines using Nunclon Sphera cell culture plastics and Gibco Media. See the protocols
When performing wound healing assays, or scratch assays, to determine the migratory potential of cancer cells, it is important to have a growth factor that does not degrade in cell culture conditions. Native basic fibroblast growth factor degrades rapidly at 37°C, adding undesired variability. Gibco Heat Stable bFGF is a superior solution to the problem by maintaining ≥ 80% bioactivity at 37°C for at least 72 hours. See the products
Gibco PB-MAX Karyotyping Medium is a fully supplemented medium developed for the short-term culture of peripheral blood lymphocytes for cytogenetic studies and in vitro diagnostic procedures. See the product
Gibco MarrowMAX Bone Marrow Medium is a fully supplemented medium developed for the short-term culture of bone marrow and other hematopoietic cells for cytogenetic studies and in vitro diagnostic procedures. See the product
A convenient list of recommended products designed for successful culturing of 2D, 3D, and co-culture cancer cell models.
| Cell types to consider | Your choice of cancer cell line across tissue types | |
| Estimated time to culture | Models ready for analysis after 3–4 days | |
| Recommended products | Media & reagents | |
| Plastics & matrices | ||
Transfection reagents |
| |
| Instruments | ||
| Cell types to consider | Your choice of cancer cell line across tissue types | |
| Estimated time to culture | Models ready for analysis after 2 weeks | |
| Recommended products | Media & reagents | |
| Plastics & matrices | ||
Transfection reagents |
| |
| Instruments | ||
| Cell types to consider | Patient tissue Your choice of iPSC line | |
| Estimated time to culture | Models ready for analysis after 1–2 months | |
| Recommended products | Media & reagents | |
| Plastics & matrices | ||
Transfection reagents |
| |
| Instruments | ||
| Cell types to consider | Cell line of choice plus cancer-associated fibroblast, immune cell line | |
| Estimated time to culture | Models ready for analysis after 3–4 days | |
| Recommended products | Media & reagents | |
| Plastics & matrices | ||
Transfection reagents |
| |
| Instruments | ||
| Cell types to consider | Your choice of cancer cell line across tissue types | |
| Estimated time to culture | Models ready for analysis after 3–4 days | |
| Recommended products | Media & reagents | |
| Plastics & matrices | ||
Transfection reagents |
| |
| Instruments | ||
| Cell types to consider | Your choice of cancer cell line across tissue types | |
| Estimated time to culture | Models ready for analysis after 2 weeks | |
| Recommended products | Media & reagents | |
| Plastics & matrices | ||
Transfection reagents |
| |
| Instruments | ||
| Cell types to consider | Patient tissue Your choice of iPSC line | |
| Estimated time to culture | Models ready for analysis after 1–2 months | |
| Recommended products | Media & reagents | |
| Plastics & matrices | ||
Transfection reagents |
| |
| Instruments | ||
| Cell types to consider | Cell line of choice plus cancer-associated fibroblast, immune cell line | |
| Estimated time to culture | Models ready for analysis after 3–4 days | |
| Recommended products | Media & reagents | |
| Plastics & matrices | ||
Transfection reagents |
| |
| Instruments | ||
Gibco cell culture is critical to your success. Gibco Cancer Basics is an introduction to cancer cell culture, covering topics such as the biology of cancer, cell line culture, and the culture of cancer spheroids and cancer organoids. Download our free cancer culture handbook featuring an overview of specific cancers and the ten hallmarks of cancer.
View these cancer line–specific protocols that describe conditions for consistent culture and transfection of popular breast, liver, lung, and prostate cancer cell lines.
In addition to traditional 2D cancer culture, scientists are investigating 3D models such as spheroid cultures as a more physiologically relevant model. This application note provides a background to cancer spheroid culture.
Transfection protocols for the most-studied cancer cell lines and cancer types, designed to help you get the results you want the first time with minimal optimization. Find your cell line–specific protocol for breast, lung, prostate, or liver cancer. The new protocols provide you with a step-by-step guide to help ensure high transfection efficiency with low toxicity for your specific cancer cell line.
Gibco Cell Culture Heroes spotlights PhD and postdoc researchers across the globe with the primary focus of telling the story of their research through global webinars. This program promotes education and creates a community amongst researchers who are driving tomorrow's breakthroughs using cell culture.
Gibco cell culture is critical to your success. Gibco Cancer Basics is an introduction to cancer cell culture, covering topics such as the biology of cancer, cell line culture, and the culture of cancer spheroids and cancer organoids. Download our free cancer culture handbook featuring an overview of specific cancers and the ten hallmarks of cancer.
View these cancer line–specific protocols that describe conditions for consistent culture and transfection of popular breast, liver, lung, and prostate cancer cell lines.
In addition to traditional 2D cancer culture, scientists are investigating 3D models such as spheroid cultures as a more physiologically relevant model. This application note provides a background to cancer spheroid culture.
Transfection protocols for the most-studied cancer cell lines and cancer types, designed to help you get the results you want the first time with minimal optimization. Find your cell line–specific protocol for breast, lung, prostate, or liver cancer. The new protocols provide you with a step-by-step guide to help ensure high transfection efficiency with low toxicity for your specific cancer cell line.
Gibco Cell Culture Heroes spotlights PhD and postdoc researchers across the globe with the primary focus of telling the story of their research through global webinars. This program promotes education and creates a community amongst researchers who are driving tomorrow's breakthroughs using cell culture.
Let nothing stand in the way of your next discovery. We at Thermo Fisher Scientific propose a bold new path forward to empower your next discovery. Explore our cancer research resources.
Each quarter, we award scientists with our Cancer Research Foundation grants. The products included in these grants will help enable their pursuits to better understand and treat cancer.
Less wasteWhen minimizing waste in your lab, every little bit helps. The 1 L Gibco bottle uses 38% less source material (32% less for the 0.5 L bottle) than comparable media bottles on the market today. This reduces the consumption of non-renewable resources helping shrink your footprint.
Download the Green Fact Sheet
Learn more about sustainable solutions
Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources for better experiment planning and execution.
Media Formulation Tool
Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM, and RPMI-1640 media.
Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.
Need technical support? Contact our expert team for technical and application support of Laboratory Products
For Research Use Only. Not for use in diagnostic procedures.
