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Polyacrylamide gels

All Invitrogen precast protein gels are high quality polyacrylamide gels designed to deliver superior performance, reliability, reproducibility, and consistency of quality. Our extensive portfolio of precast protein gels provides a wide variety of gel options available for your research. Whether your protein is 2.5 kDa or as large as 500 kDa, or you’re looking for separation under native conditions or by isoelectric point—we have the right gel for your application.

Our precast protein polyacrylamide gels are offered in four different chemistries. The choice of whether to use one chemistry or another depends on the abundance of the protein you’re separating, the size of the protein, and your downstream application.

SDS PAGE protein separation

For denaturing gel electrophoresis or SDS PAGE there are many options available based on the application, and size of the protein of interest. For separation of a broad range of proteins, two chemistries, Bis-Tris and Tris-glycine, have been optimized for performance and long shelf life. Choose Bis-Tris gel chemistry when you have a low abundance of protein or when your downstream applications require high protein integrity, such as posttranslational modification analysis, mass spectrometry, or sequencing. Bis-Tris chemistry provides a neutral (pH 7.0) environment during electrophoresis, which may result in better sample integrity and stability of the gel. This helps reduce protein modifications and helps produce sharp band resolution. Bis-Tris gel chemistry is offered in our Bolt Bis-Tris Plus and NuPAGE Bis-Tris gels. Bolt Bis-Tris Plus mini gels build on the legacy of the highly published NuPAGE Bis-Tris gels, feature larger capacity wells- for loading up to 60 µl per well, and can be run in as little as 20 minutes.

To separate high-abundance proteins, select our robust Novex Tris-Glycine gel chemistry, which offers maximum protein separation performance and crisp, straight bands.

For separation of high molecular weight proteins, Tris-acetate gel chemistry is recommended, offered in NuPAGE Tris-Acetate gels.

For separation of low molecular weight proteins, use Novex Tricine gels which provide increased resolution of proteins with molecular weights as low as 2.5 kDa.

Native PAGE protein separation

For separation of proteins under nondenaturing conditions or native page, three gel systems are recommended- NativePAGE Bis-Tris, Novex Tris-Glycine and NuPAGE Tris-Acetate. NativePAGE Bis-Tris gels, are based on the blue native polyacrylamide gel electrophoresis (BN-PAGE) which helps overcome the limitations of traditional native electrophoresis by providing a near-neutral operating pH and detergent compatibility. Both Novex Tris-Glycine and NuPAGE Tris-Acetate gels can be run under native and non-reducing conditions for separation of proteins by net charge, size and shape.

For separation of proteins by isoelectroctric point (pI), use Novex IEF gels. Novex IEF gels effectively create a pH gradient to separate proteins according to their unique pI.