Thermo Scientific Zeba Spin Desalting Columns are made of low protein binding polypropylene and compatible with a wide range of standard laboratory instruments and consumables.
Our Zeba columns are designed to be compatible with most swinging-bucket or fixed-angle bench- or floor-model centrifuges (please verify head clearance before use), and are available in five volume capacities and two molecular weight cut-offs (MWCOs), 7K and 40K.
Choose the right Zeba Spin Desalting Column for you
 |  |  |  |  |  | |
| Format | Micro Spin Column | 0.5 mL Spin Column | 2 mL Spin Column | 5 mL Spin Column | 10 mL Spin Column | 96-well Filter Plates |
| Resin bed volume | 75 μL | 0.5 mL | 2 mL | 5 mL | 10 mL | N/A |
| Volume capacity (7K MWCO) | 2–12 μL | 30–130 μL | 200–700 μL | 500–2,000 μL | 700–4,000 μL | 20–100 µL |
| Volume capacity (40K MWCO) | 5–14 μL | 70–200 μL | 200–900 μL | 300–2,000 μL | 1,000–4,000 μL | 20–100 µL |
| 7K MWCO pack sizes Cat. No. | 89877, 89878 | 89882, 89883 | 89889, 89890 | 89891, 89892 | 89893, 89894 | 89807, 89808 |
| 40K MWCO pack sizes Cat. No. | A57756, A57758 | A57759, A57760 | A57761, A57762 | A57763, A57764 | A57765, A57766 | A57767, A57768 |
Compare Zeba spin desalting columns
Table 1. Comparison of recommended sample volume capacity of common spin desalting products.
Protein recovery comparison using Zeba Protein Desalting Columns with 7K and 40K MWCO resin
Redeveloped Zeba 40K Spin Desalting Columns demonstrate improved protein recovery for a variety of proteins in the 40 kDa and above molecular weight range.
| Recovery* | |||
|---|---|---|---|
| Protein | Molecular Size | 7K Zeba Column | 40K Zeba Column |
| Bacitracin | 1.5 kDa | 15% | 12% |
| Aprotinin | 6.5 kDa | 58% | 37% |
| Ubiquitin | 8.5 kDa | 78% | 51% |
| Lactalbumin | 14 kDa | 88% | 64% |
| Trypsin Inhibitor | 21 kDa | 94% | 76% |
| Carbonic Anhydrase | 30 kDa | 90% | 79% |
| Ovalbumin | 42 kDa | 95% | 95% |
| BSA | 67 kDa | 94% | 93% |
| Rabbit IgG | 150 kDa | 92% | 92% |
*Recovery of 100 uL sample volume, with a loaded concentration of 0.5 mg/mL
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