Breakout tools for standout work
Get ultimate flexibility and speed for pivotal results with Ion AmpliSeq panels
When navigating through the complexities of genetic diseases, you need a discovery tool that is both flexible and rapid for analyzing variants of interest. Ion AmpliSeq technology can help.
Ion AmpliSeq technology is globally recognized as the gold-standard, amplicon-based enrichment method for targeted next-generation sequencing (NGS). For the fastest, simplest, and most scalable NGS solution, there is no substitute.
NEW Custom Ion AmpliSeq assays on the Genexus Integrated Sequencer are now compatible with using up to 48 barcodes per run for increased sample throughput and with the Ion Torrent GX7 Chip for greater sequencing capacity.
Available in Ready-to-Use, Made-to-Order, and On-Demand formats, Ion AmpliSeq panels consist of a pool of oligonucleotide primer pairs, with each pair designed to amplify a specified genomic region. Each panel can be designed to interrogate all bases across a gene, or it can be focused on specific mutation hotspots.
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To browse panels, visit ampliseq.com
Gene panels on demand, how and when you want them
Ion AmpliSeq On-Demand panels bring smaller pack sizes and an optimized catalog of pre-tested genes to custom NGS panels, so you can iterate panel design for human disease research with efficiency and convenience.
Not working with human research samples? No problem.
Design a custom panel from 13 predefined genomes, or from your own reference genome after uploading a reference file.
One tube for CNV, SNP, indel, and fusion detection
Simple, versatile technology based on highly multiplexed PCR helps you target tens to hundreds of genes across mutation types including SNPs, indels, fusions, and copy number variants.
What can you do with NGS using Ion AmpliSeq panels? Get results.
After more than 25,000 custom designs and 1000 publications, custom Ion AmpliSeq panels have a track record of superior coverage uniformity, reproducibility, and specificity. They help you get results.
Need even higher sensitivity?
To design your own panels and find variants with a very low limit of detection (down to 0.1%), try Ion AmpliSeq HD technology. This novel library amplification technology enables researchers to get answers when ultrahigh sensitivity is required, such as when detecting low-frequency alleles in circulating tumor DNA or trace pathogenic microbial species in blood.
Ion AmpliSeq panels
On-Demand panels
Ion AmpliSeq On-Demand panels enable custom panel design in smaller pack sizes from pre-tested genes for practical customization that helps reduce upfront cost and risk.
RNA-seq panels
Choose from over 20,000 genes to survey gene expression fold changes starting with as little as 500 pg of unfixed RNA or 5 ng of FFPE RNA.
Made-to-order
For maximum flexibility, simply select a subset of targets and enter your specific genomic content online using the Ion AmpliSeq Designer tool, and we will design and create customized Ion AmpliSeq panels just for you. Choose from a number of reference genomes or upload your own reference sequence.
Transcriptome panels
The Ion AmpliSeq Transcriptome Human Gene Expression Kit is ideal for traditional gene expression analysis of known transcripts from the RefSeq database.
Ready-to-use panels
The convenient predesigned Ion AmpliSeq ready-to-use panels allow researchers to focus on data generation and analysis, not on the labor-intensive primer design and target selection steps.
Exome panels
The Ion AmpliSeq Exome RDY S5 Kit allows for rapid, accurate sequencing of key exonic regions of the genome, going from DNA to variants in just 2 days.
Ion AmpliSeq panels by application
Deafness, epilepsy, cardiac research, and more
Cancer genomics, cancer RNA expression, and more
Tracing the origin of outbreaks, food safety, and more
DNA phenotyping, ancestry, and more
Ion Torrent next-generation sequencing systems
Scalable targeted NGS to support small and large projects
The Ion GeneStudio S5 system is a scalable, targeted-NGS workhorse with wide application breadth and throughput capability.
Specimen to report in a single day with a hands-off, automated workflow*
The Genexus System is the first turnkey NGS solution that automates the specimen-to-report workflow and delivers results in a single day with just two user touchpoints.*
Ion AmpliSeq panel results from Boston Children’s Hospital, USA
Applications of targeted NGS panels for clinical research of primary immunodeficiency diseases
Dr. Janet Chou and Wayne Bainter discuss the application of the Ion AmpliSeq Primary Immune Deficiency (PID) Research Panel v2 to accelerate and improve the search for causal variants in clinical research of PIDs.
Videos to help you get started
How does Ion AmpliSeq technology work?
Transitioning from Sanger sequencing? It’s easy to get started.
For Research Use Only. Not for use in diagnostic procedures.