Immunofluorescent staining using mouse anti-Golgin-97 monoclonal antibody

Golgi and actin staining in HeLa cells. Golgi in fixed and permeabilized HeLa cells labeled with anti–golgin-97 monoclonal antibody (Cat. No. A21270) and visualized with green-fluorescent Alexa Fluor® 488 Goat-Anti–Mouse IgG (Cat. No. A11001). Actin was stained with red-fluorescent Alexa Fluor® 594 phalloidin (Cat. No. A12381); nuclei were stained with blue-fluorescent DAPI (Cat. No. D1306, D3571, D21490). Treatment with Image-iT® FX signal enhancer (Cat. No. I36933) largely eliminates nonspecific dye binding (bottom) as compared to untreated slide (top).

Related Images

A prometaphase muntjac skin fibroblast stained with Alexa Fluor® 350 phalloidin, an anti–a-tubulin antibody and an anti–cdc6 peptide antibody. Go ›

Bovine pulmonary artery endothelial cells (BPAEC). MitoTracker® Red CMXRos, SYTOX® Green nucleic acid stain, biotin-XX goat anti–mouse IgG antibody and Cascade Blue® NeutrAvidin biotin-binding protein. Go ›

Endothelial cells. Go ›

Mouse Anti-Alpha Tubulin Monoclonal Antibody (Cat. No. A11126) Go ›

1% Agarose gel containing 16S and 23S ribosomal RNA (rRNA). SYBR® Green II RNA gel stain. Go ›

Fixed and stained HDFn cells. Go ›

Simultaneous detection of expression of five genes in a whole mount Drosophila embryo by fluorescence in situ hybridization (FISH) with five RNA probes Go ›